Genetic mapping of the three-pistil gene Pis1 in an F2 population derived from a synthetic hexaploid wheat using multiple molecular marker systems

Morphological characterization and genetic analysis of tri-pistil trait, a precisely regulated pistil number mutation in bread wheat

“…Given that TRP was a single dominant gene, additionally, the 5A locus was only detected in one of the replicate penetrance traits (Supplemental Table 4). Thus, the 5A locus may have been a pseudo-locus, since it Mapping results were consistent with previous studies (Mahlandt et al 2021;Yu et al 2020).…”

“…Seeds were saved and developed into a pure wheat line, referred to as the "tri-grain wheat". Within the past fty years, studies evaluated oral morphologies, genetic make-up, biochemical features, and gene mapping of similar tri-pistil mutants, which were subsequently referred to as "multi-ovary" (MOV), "multiple-pistil" (MP), and "three-pistil" (TP) (Chen et Yu et al 2020). The tri-pistil mutants generate normal stamens and other oral organs, except for three fertile pistils (Li et al 2020c; Mahlandt et al 2021;Peng 2003).…”

“…Mahlandt et al 2021;Yu et al 2020). In addition, poor penetrance of most 4045 F 1 populations, which differed from previous studies, implied that TRP may be different from Pis1 or Mov-1.Map-based cloning is an important strategy for gene identi cation in wheat, whereas it was di cult and time-consuming before the release of wheat genomes.…”

Investigation of Thermomorphogenesis-Related Genes for a Multi-Silique Trait in Brassica napus by Comparative Transcriptome Analysis

“…However, similar phenomena described as "multi-pistil" have been reported in wheat (Triticum aestivum) (Yang et al, 2011(Yang et al, , 2017Duan et al, 2015;Wei, 2017;Guo et al, 2019;Zhu et al, 2019;Yu et al, 2020), rice (Oryza sativa) (Zheng et al, 2019), sweet cherry (Prunus avium) (Liu et al, 2019;Wang et al, 2019), and alfalfa (Medicago sativa) (Nair et al, 2008). In wheat, increasing the number of grains per spike is considered to be vital for maximizing its yield potential (Wei, 2017;Zhu et al, 2019;Yu et al, 2020). The multi-pistil traits in wheat were found to be controlled by a recessive gene, two recessive non-complementary genes, or a single dominant gene; F 2 populations, BC 6 F 2 populations, or near-isogenic lines (NILs) were constructed to map the underlying locus, and they found to be located on 2DL, 5DS, 6BS, and 6B (Zhu et al, 2019).…”

“…This trait is different from the multilocular phenotype in Brassica plants, such as tetra-locular Brassica rapa (Yadava et al, 2014;Lee et al, 2018), multilocular Brassica juncea (Xiao et al, 2013;Xu et al, 2014), etc., which increases the number of locules in a silique. However, similar phenomena described as "multi-pistil" have been reported in wheat (Triticum aestivum) (Yang et al, 2011(Yang et al, , 2017Duan et al, 2015;Wei, 2017;Guo et al, 2019;Zhu et al, 2019;Yu et al, 2020), rice (Oryza sativa) (Zheng et al, 2019), sweet cherry (Prunus avium) (Liu et al, 2019;Wang et al, 2019), and alfalfa (Medicago sativa) (Nair et al, 2008). In wheat, increasing the number of grains per spike is considered to be vital for maximizing its yield potential (Wei, 2017;Zhu et al, 2019;Yu et al, 2020).…”