Genetic Variability in Lathyrus spp. as Revealed by Karyotype Analysis

Badr, Salwa; Mustafa, Abd El-Zahar; Taher, Walla; Sammour, R. H.

doi:10.1508/cytologia.74.101

Cited by 6 publications

(5 citation statements)

References 18 publications

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“…Similar karyotype formulae were reported by Badr et al (2009) for different germplasms of Lathyrus sativus collected from Canada, Afghanistan and Hungary. No gradual decrease in individual chromosome length was found in these three BARI varieties.…”

Section: Karyotype Analysissupporting

confidence: 83%

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Characterization of Three Varieties of Lathyrus sativus L. by Fluorescent Karyotype and RAPD Analysis

et al. 2015

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Summary Three varieties of Lathyrus sativus L. released from Bangladesh Agricultural Research Institute (BARI), viz., BARI Khesari-1, BARI Khesari-2 and BARI Khesari-3, were studied cytogenetically and at the molecular level by using RAPD for genomic characterization. The three varieties were found to posses 2n=14 chromosomes. The karyotype formulae of BARI Khesari-1 and BARI Khesari-2 are 8m + 6sm, while it is 10 m + 4sm in BARI Khesari-3. After orcein and CMA staining, one to two very small chromosome-like bodies were found in some cells of BARI Khesari-1, whereas no such body was observed after DAPI staining. Due to their unique features these can be considered as GC-rich heterochromatic B-chromosomes. In BARI Khesari-2, two pairs of satellites were found, while only one pair was present in BARI Khesari-3. The inverted position of satellited regions indicated the probable occurrence of homozygous inversion. An indication of paracentric inversion regarding DAPI-banding pattern was found in pair V of BARI Khesari-2 and thus could be considered as marker chromosomes for this variety. The three varieties have distinct CMA-and DA-PI-banding patterns. In BARI Khesari-2, a total of 12 C-bands were found on 10 chromosomes (out of 14 chromosomes). On the basis of band position and length, a deep correlation between C-banding and DAPI-banding pattern was found suggesting the heterochromatic nature of DAPI bands. Each variety showed different RAPD fingerprinting with 59.09% polymorphisim. In addition, a number of variety-specific unique RAPD bands was observed. Therefore, each variety could be characterized on the basis of karyotype and RAPD analysis.

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Section: Karyotype Analysissupporting

confidence: 83%

“…The above features suggest that this chromosome-like body is a B-chromosome . Badr et al (2009) confidently reported Bchromosome in Lathyrus sativus. Generally, B-chromosomes are heterochromatic in nature.…”

Section: B-chromosomes?mentioning

confidence: 97%

Characterization of Three Varieties of Lathyrus sativus L. by Fluorescent Karyotype and RAPD Analysis

et al. 2015

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“…After the germination of spores dikaryotic infectious hyphae enter in the germinating seed. Therefore, fungal pathogens start growing through the seed coat into the scutellum and embryo at the base of the seed (Badr et al 2009;Sammour et al 2015). Once mycelia grow in the developing seed in start penetrating and reached till the top position in crop, initially these pathogens travel slowly and eventually develops into a smutted ear (Abrahim, 2019).…”

Section: Methodsmentioning

confidence: 99%

Identification of Isolated Strains of Ustilago Tritici Rs-01 From an Infected Wheat Crop

Badar,

Ahmed,

Kanwal

et al. 2023

Pak. J. Phytopathol.

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Loose smut has a wide distribution and can occur anywhere wheat is cultivated and produced. It is caused by the fungus Ustilago tritici. Mild symptoms may be present prior to heading, including yellowish leaf streaks and stiff, dark green leaves. Spores are not enclosed by the seed coat, so are quickly dispersed by rain or wind after emergence. These spores infect other wheat plants at flowering, causing seed infection. Wheat (Triticum aestivum L.) is an important crop and staple food of human population throughout the World. It is widely cultivated in Pakistan and in most of the other agricultural countries like China, India and Bangladesh. Therefore, an experimental study was designed to cultivate Galaxy-2013 for the isolation of pathogenic strains of tritici having potential to cause loose smut of wheat, after natural attack during cropping year 2019-2020. Main purpose of study was to isolate samples of Ustilago, which would be used for the testing of anti-fungal products for future studies during cropping years (2020-2022) after complete identification, isolated strains (RS01) were identified as Ustilago tritici and an accession number (OP164708_RS01) was obtained after blast the obtained sequences at NCBI, which were further confirmed by phylogenetic analysis. Therefore, it was expected that some wheat varieties those are vulnerable to the attack of Ustilago required careful cultivation by adapting suitable strategies to protect crops especially wheat from certain disease to enhance food requirement in the country.

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“…The above features suggest that these chromosome-like bodies are B-chromosomes . B-chromosomes were reported in different species such as Lathyrus sativus L. (Badr et al 2009) and Datura metal L. (Alam and Begum 2001). Since there is no chromosomal information available for T. chantrieri, it was not possible to compare the presence of B-chromosomes in this species.…”

Section: B-chromosome?mentioning

confidence: 99%

Cyto-Molecular Characterization of Three Tacca Species from Bangladesh

2016

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Summary Three species of Tacca J. R. & G. Forst., namely T. plantaginea, T. chantrieri and T. integrifolia, were investigated cytogenetically and at the molecular level to solve their taxonomic rank. The diploid chromosome number 2n=28 was found in T. plantaginea and T. integrifolia whereas 2n=22 was found in T. chantrieri. This is probably the first report on the diploid chromosome number of T. plantaginea and T. chantrieri around the world. The three species differed in respect of centromeric formulae such as 22m+6sm in T. plantaginea, 18m+4sm in T. chantrieri and 28m in T. integrifolia. In addition to regular 2n=22 chromosomes, four to eight small chromosomes were found in some cells of T. chantrieri suggesting the probable occurrence of B-chromosome. In T. integrifolia, a pair of satellites was found on the short arm, one in each member of pair VI, after orcein and CMA staining but not in DAPI, revealing the GC-rich nature. A wide range of CMA-and DAPI-positive bands was found in the metaphase chromosomes of the three species. DAPIfluoresced chromosomes were frequent in these three species. Six primer combinations were applied for RAPD analysis in the three species of Tacca to find out their genomic relationship. The three species showed some unique bands useful as markers for each species. Conventional and fluorescent karyotype together with RAPD fingerprinting indicated that T. integrifolia is distantly related to T. plantaginea and T. chantrieri. Therefore, the compilation of the above cytological and molecular information will be very useful for authentic identification of the three Tacca species. This genetic information will help in the proper conservation of and to make patent for the three threatened Tacca species found in Bangladesh.

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Genetic Variability in Lathyrus spp. as Revealed by Karyotype Analysis

Cited by 6 publications

References 18 publications

Characterization of Three Varieties of <i>Lathyrus sativus</i> L. by Fluorescent Karyotype and RAPD Analysis

Characterization of Three Varieties of <i>Lathyrus sativus</i> L. by Fluorescent Karyotype and RAPD Analysis

Identification of Isolated Strains of Ustilago Tritici Rs-01 From an Infected Wheat Crop

Cyto-Molecular Characterization of Three <i>Tacca</i> Species from Bangladesh

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