This work aimed at exploring the genetic variability, population structure and relationships of Lathyrus sativus L. germplasm using isozyme analysis. The data of isozyme analysis revealed 12 putative polymorphic loci of a total 33 alleles, indicating that studied accessions express good allelic richness and had an apparent rate of allogamy. The mean average of the expected heterozygosity (0.483) was more than the mean average of the observed heterozygosity (0.449) suggesting an apparent rate of allogamy taking place in Lathyrus sativus L. The average of total heterozygosity (HT) and intra-accessional genetic diversity (HS) were 0.559 and 0.428 respectively indicating that majority of genetic diversity was intra-accessional. The low levels and non-significant of genetic diversity among accessions (DST = 0.190, X2 = 62.59, p = 0.029) were probably indicative of occurrence of several gene flows. The coefficient of gene differentiation (GST) showed significant variation, suggesting the heterogonous distribution of L. sativus accessions among different geographic regions. The mean average of FST was 0.327, suggesting the occurrence of random mating system for the studied accessions and reflecting adaptation to strong environmental dissimilarities. Cluster analysis based on isozyme data suggested that the environment had no influence on the genetic diversity and confirmed that Lathyrus sativus L. had a polyphyletic origin.

SummaryThe genetic variability based on karyotype formula was studied for 40 accessions of genus Lathyrus. All accessions have 2nϭ14 chromosomes. Karyotype formula has a great uniformity among accessions of each species, suggesting interaspecific stability in Lathyrus species. Our data allowed the differentiation of several accessions among sections Lathyrus, Linearicarpus and Clymenum in number of m chromosome. The variation among section Lathyrus supports that section Lathyrus is not fully constant as has been postulated. Satellites were detected in a pair of chromosome in L. sativus (TUN and AFG). On the otherhand, B-chromosome was detected in L. sativus (USSR, BAN, CAN, and PAK) L. gorgoni (JOR) and L. annus (SYR). This finding may be due to the variation in DNA amount by increase in the non-repetitive sequence. The variation in size of chromosomes of the members of Lathyrus is associated with the evolution in the genus.

No abstract

Anchovy is a main capture fishery commodity in North Maluku. Currently, the processing activity of anchovy is relatively simple and traditional, consequently, causing anchovy quality to be easily degraded. One solution to overcome the problem is through the addition of chitosan concentration. This study aimed to determine effect of concentration addition of chitosan on quality of water content, protein, fat, carbohydrates, organoleptic and Total Plate Count (TPC) test. Method used in this research was the analysis of water quality test, protein content, fat content, carbohydrate content, calorie content, organoleptic test and TPC test. The study results showed that the highest water content value was 13.65% at 0.1% concentration, the highest protein value was 61.16% at 0.5% concentration, the highest ash content value was 16.5% at 1% concentration, the highest fat value was 3.99% at 1% concentration, and the highest fat value was 3.99% at 1% concentration. The highest carbohydrate content found was 11.94% at 0.1% concentration, and the highest calorific value was 2.51% at 1% concentration. Meanwhile, average organoleptic value of the highest appearance was 6.87% at a 0.3% concentration, the highest odor was 7.55% at a 0.3% concentration, the highest texture was 7.45% at a 0.1% concentration and color was 6.25% at a 0.3% concentration. Results of the TPC tests showed that anchovy with chitosan treatment and without chitosan during storage changed for all treatments. The average TPC value at all concentrations (0.1%, 0.3% and 0.5%) had the lowest TPC value compared to the control.