Genistein Induces p21Cip1/WAF1Expression and Blocks the G1 to S Phase Transition in Mouse Fibroblast and Melanoma Cells

Kuzumaki, Takejiro; Kobayashi, Takashi; Ishikawa, Kiichi

doi:10.1006/bbrc.1998.9462

Cited by 74 publications

(36 citation statements)

References 17 publications

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“…We also checked the expression of p21, a cell cycle-related gene involved in the effects of flavonoids on cell proliferation. [31][32][33][34][35] We found that p21 (GeneBank ID BE282021), despite not being classified in the first screening of two-fold change cutoff, was 1.41-fold upregulated.…”

Section: Effects Of Gspe On Differentiation Markersmentioning

confidence: 90%

“…Genistein, a soy flavonoid, was shown to inhibit both proliferation and differentiation in 3T3-L1 when added at the induction of differentiation, 6 and this induced growth arrest was accompanied by an increase in p21 expression and subsequent cyclin E/CDK 2 supression. 31 In fact, several Antiadipogenic effects of procyanidins M Pinent et al flavonoids have been shown to exert growth-inhibitory effects through upregulation of p21 in different cell lines. [32][33][34][35] We also found an upregulation of p21 by GSPE treatment at day 0.…”

Section: Discussionmentioning

confidence: 99%

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Grape-seed derived procyanidins interfere with adipogenesis of 3T3-L1 cells at the onset of differentiation

et al. 2005

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OBJECTIVE: Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-g (PPARg) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARg2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells. DESIGN: We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h. MEASUREMENTS: Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique). RESULTS: Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE. CONCLUSION: These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.

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Section: Effects Of Gspe On Differentiation Markersmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Grape-seed derived procyanidins interfere with adipogenesis of 3T3-L1 cells at the onset of differentiation

et al. 2005

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“…p53 and p21/waf1 have been extensively studied as inducers of tumor cell apoptosis and bcl-2 as an important apoptosis repressor. It has been reported that genistein induces apoptosis of several types of tumor cells in vitro, including melanoma cells [21,22], breast cancer cells [23,24], and prostate cancer cells [25,26], by means of induction of p21/waf1 [21]. Genistein also induces apoptosis of human breast cancer cells in vitro by inactivating bcl-2 and inducing the expression of p53 [27].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of orthotopic growth and metastasis of androgen‐sensitive human prostate tumors in mice by bioactive soybean components

Zhou

Zhong

et al. 2002

The Prostate

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BACKGROUND-Systematic analysis of the influence of diet on the initiation and progression of prostate cancer is often difficult in human populations, for which dietary variables overlap a diversity of genetic backgrounds and social behaviors. Animal models that emulate human prostate cancer allow experimental analysis of the mechanisms of action of nutritional agents that show anti-prostate cancer activity.

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“…However, the effect of genistein was more pronounced in malignant cells compared to normal cells. Thus, it is generally accepted that genistein can cause G2/M cell cycle arrest, but a report has shown that genistein could also arrest mouse fibroblast and melanoma cells at G0/G1 phase of the cell cycle [36]. These data suggests that genistein induces either G2/M or G0/G1 cell cycle arrest, depending on cell lines.…”

Section: Effects On Cell Cycle Regulationmentioning

confidence: 99%

Multi-targeted therapy of cancer by genistein

Banerjee¹,

Li²,

Wang³

et al. 2008

Cancer Letters

561

383

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Soy isoflavones have been identified as dietary components having an important role in reducing the incidence of breast and prostate cancers in Asian countries. Genistein, the predominant isoflavone found in soy products, has been shown to inhibit the carcinogenesis in animal models. There is a growing body of experimental evidence showing that the inhibition of human cancer cell growth by genisteinis mediated via the modulation of genes that are related to the control of cell cycle and apoptosis. It has been shown that genistein inhibits the activation of NF-κB and Akt signaling pathways, both of which are known to maintain a homeostatic balance between cell survival and apoptosis. Moreover, genistein antagonizes estrogen-and androgen-mediated signaling pathways in the processes of carcinogenesis. Furthermore, genistein has been found to have antioxidant properties, and shown to be a potent inhibitor of angiogenesis and metastasis. Taken together, both in vivo and in vitro studies have clearly shown that genistein, one of the major soy isoflavones, is a promising agent for cancer chemoprevention and further suggest that it could be an adjunct to cancer therapy by virtue of its effects on reversing radioresistance and chemoresistance. In this review, we attempt to provide evidence for these preventive and therapeutic effects of genistein in a succinct manner highlighting comprehensive state-of-the-art knowledge regarding its multi-targeted biological and molecular effects in cancer cells.

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Genistein Induces p21Cip1/WAF1Expression and Blocks the G1 to S Phase Transition in Mouse Fibroblast and Melanoma Cells

Cited by 74 publications

References 17 publications

Grape-seed derived procyanidins interfere with adipogenesis of 3T3-L1 cells at the onset of differentiation

Grape-seed derived procyanidins interfere with adipogenesis of 3T3-L1 cells at the onset of differentiation

Inhibition of orthotopic growth and metastasis of androgen‐sensitive human prostate tumors in mice by bioactive soybean components

Multi-targeted therapy of cancer by genistein

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