Genome Sequence of Cronobacter sakazakii Myovirus vB_CsaM_GAP31

Abbasifar, Reza; Kropinski, Andrew M.; Sabour, Parviz M.; Ackermann, Hans-Wolfgang; Villa, Argentina Alanis; Abbasifar, Arash; Griffiths, Mansel W.

doi:10.1128/jvi.02629-12

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…In contrast, we have shown here that phage S144 is able to infect C. sakazakii, in addition to specific Salmonella serovars. Interestingly, the C-terminus of the tail fiber of the Loughboroughvirus phages (S144, SE4 and ZCSE2) is more similar to the otherwise unrelated Cronobacter phages GAP31 and GAP32 [71,72] than to the Rosemountvirus phages. While phages SE4 and ZCSE2 have been proven to infect many different Salmonella serovars, no bacterial strains from other genera were tested [35,43].…”

Section: Discussionmentioning

confidence: 97%

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

Gambino

Sørensen

Ahern

et al. 2020

IJMS

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Phages are generally considered species- or even strain-specific, yet polyvalent phages are able to infect bacteria from different genera. Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae strains, we found that phage S144 forms plaques on specific serovars of Salmonella enterica subsp. enterica and on Cronobacter sakazakii. Analysis of phage resistant mutants suggests that the O-antigen of lipopolysaccharide is the phage receptor in both bacterial genera. The S144 genome consists of 53,628 bp and encodes 80 open reading frames (ORFs), but no tRNA genes. In total, 32 ORFs coding for structural proteins were confirmed by ESI-MS/MS analysis, whereas 45 gene products were functionally annotated within DNA metabolism, packaging, nucleotide biosynthesis and phage morphogenesis. Transmission electron microscopy showed that phage S144 is a myovirus, with a prolate head and short tail fibers. The putative S144 tail fiber structure is, overall, similar to the tail fiber of phage Mu and the C-terminus shows amino acid similarity to tail fibers of otherwise unrelated phages infecting Cronobacter. Since all phages in the Loughboroughvirus genus encode tail fibers similar to S144, we suggest that phages in this genus infect Cronobacter sakazakii and are polyvalent.

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Section: Discussionmentioning

confidence: 97%

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

Gambino

Sørensen

Ahern

et al. 2020

IJMS

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“…70%) of its genes were ORFans [67,68]. Since then five other phages have been reported to be rV5-like: coliphages vB_EcoM-FV3 [69], phAPEC8 [70] and phi92 [59], Cronobacter sakazakii phage vB_CsaM_GAP31 [71] and Salmonella phage PVP-SE1 [39]. To this list we can also add Salmonella phage SSE-121 (JX181824); and, coliphage Delta Y that Andrey Letarov and Alla Golomidova (Winogradsky Institute of Microbiology, RAS, Moscow, Russia), isolated from horse manure, and partially sequenced.…”

Section: Discussionmentioning

confidence: 99%

The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

Kropinski

Waddell

Meng

et al. 2013

Virol J

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BackgroundBacteriophages (phages) have been used extensively as analytical tools to type bacterial cultures and recently for control of zoonotic foodborne pathogens in foods and in animal reservoirs.MethodsWe examined the host range, morphology, genome and proteome of the lytic E. coli O157 phage rV5, derived from phage V5, which is a member of an Escherichia coli O157:H7 phage typing set.ResultsPhage rV5 is a member of the Myoviridae family possessing an icosahedral head of 91 nm between opposite apices. The extended tail measures 121 x 17 nm and has a sheath of 44 x 20 nm and a 7 nm-wide core in the contracted state. It possesses a 137,947 bp genome (43.6 mol%GC) which encodes 233 ORFs and six tRNAs. Until recently this virus appeared to be phylogenetically isolated with almost 70% of its gene products ORFans. rV5 is closely related to coliphages Delta and vB-EcoM-FY3, and more distantly related to Salmonella phages PVP-SE1 and SSE-121, Cronobacter sakazakii phage vB_CsaM_GAP31, and coliphages phAPEC8 and phi92. A complete shotgun proteomic analysis was carried out on rV5, extending what had been gleaned from the genomic analyses. Host range studies revealed that rV5 is active against several other E. coli.

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“…However, before application, it is necessary to obtain sufficient information about the use of a particular phage as a controlling agent to guarantee its safety. Presently, only nine Cronobacter phages have been reported, including five myoviruses (GAP31, GAP161, ESSI-2, ES2, and CR3) (6–10), three siphoviruses (ESP2949-1, phiES15, and ENT39118) (11–13), and the unclassified phage ENT47670 (GenBank accession no. HQ201308).…”

Section: Genome Announcementmentioning

confidence: 99%

The Genome of Cronobacter sakazakii Bacteriophage vB_CsaP_GAP227 Suggests a New Genus within the Autographivirinae

et al. 2013

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Genome Sequence of Cronobacter sakazakii Myovirus vB_CsaM_GAP31

Cited by 10 publications

References 16 publications

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

The Genome of Cronobacter sakazakii Bacteriophage vB_CsaP_GAP227 Suggests a New Genus within the Autographivirinae

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