Genome-wide identification and analysis of elongase of very long chain fatty acid genes in the silkworm, <i>Bombyx mori</i>

Zuo, Weidong; Li, Chunlin; Luan, Yue; Zhang, Hao; Tong, Xiaoling; Han, Minjin; Gao, Rui; Hu, Hai; Song, Jun‐Ho; Dai, Fangyin; Lü, Cheng

doi:10.1139/gen-2017-0224

Cited by 18 publications

(11 citation statements)

References 50 publications

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“…The reason may be related to the different contents and proportions of lipids in the artificial diet and mulberry leaves, or the process of lipid metabolism in the silkworm body may have changed due to the intake of the artificial diet. Sphingolipids belong to the very long chain fatty acids, are a component of cellular lipids, and play an important role in cell proliferation (Zuo et al, 2018). Moreover, sphingolipids can promote the growth of cell neurites (Kwon et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Haemolymph metabolomic differences in silkworms (Bombyx mori L.) under mulberry leaf and two artificial diet rearing methods

ShanShan

Wang

Liu

et al. 2021

Arch Insect Biochem Physiol

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The new technology of silkworm (Bombyx mori L.) artificial feed breeding has many characteristics and advantages.This study assessed silkworm rearing with mulberry leaf at all instars (MF) as the control, and used metabolomics to explore the differences in haemolymph metabolism of fifth instar silkworms under two modes of rearing with an artificial diet at all instars (AF) and rearing with an artificial diet during first to third instars and mulberry leaf during the fourth and fifth instars (AMF). The results show that, compared with silkworms of the MF group, the amount and fold change of various metabolites were higher in the haemolymph of AF group silkworms, and the metabolism of amino acids and uric acid, carbohydrates, lipids, and vitamins were changed. These changes may be the reasons for the poor performance of the AF silkworms. However, the amount and fold change of the various metabolites of silkworms in the AMF group were lower, and some metabolic pathways were more active. The amount of material and energy supply were greater. These changes could explain the high efficiency growth of body weight of silkworms after the conversion from artificial diet rearing to mulberry leaf rearing. These findings provide an important theoretical basis for the optimisation of artificial diet rearing technology for silkworms.

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Section: Discussionmentioning

confidence: 99%

Haemolymph metabolomic differences in silkworms (Bombyx mori L.) under mulberry leaf and two artificial diet rearing methods

ShanShan

Wang

Liu

et al. 2021

Arch Insect Biochem Physiol

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“…Unigene2015 (cuticular protein analogous to peritrophin with one chitin-binding domain), − Unigene16835 (hypothetical CP), Unigene29662, Unigene1363 (Cuticlin 1), and Unigene3721 (cuticular protein with Rebers and Riddiford motif) encode cuticular proteins . Unigene1442 [acyl-CoA 11-( Z )-desaturase] , and Unigene29000 (elongase of very long-chain fatty acid 4) encode enzymes possibly involved in the synthesis or modification of cuticular hydrocarbons and lipids. , Unigene10918 (laccase-2) encodes a key enzyme responsible for cuticle tanning. , Unigene2037, Unigene9511, and Unigene23358 belong to the newly discovered Osiris gene family, which is essential for development but also plays a role in phenotypic plasticity and in immunity/toxicity responses . In Tribolium castaneum, the RNAi of Osiris genes arrested the metamorphosis process and resulted in a high lethality rate …”

Section: Resultsmentioning

confidence: 99%

Molecular Insights into the Insensitivity of Lepidopteran Pests to Cycloxaprid

Liu

et al. 2020

J. Agric. Food Chem.

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Cycloxaprid (CYC) is effective in the control of hemipteran pests, but its bioactivity against lepidopteran pests is still unclear. Here, the bioactivity of CYC against lepidopteran pests was found to be much worse than that against hemipteran insects. To reveal the mechanism, the transcriptomes of CYC-treated and untreated Ostrinia furnacalis larvae were compared. Among the top 20 differentially expressed genes, 11 encode proteins involved in cuticle formation, while only one encodes a detoxifying enzyme. Thus, the cuticle appears to be important for the insensitivity of O. furnacalis to CYC. A pretreatment of O. furnacalis larvae with methoprene enhanced the bioactivity of CYC by 1.12-fold. Moreover, mixtures of CYC with graphene oxide increased the bioactivity of CYC by 1.88-fold. Because lepidopteran and hemipteran insects often harm crops at the same time, the work can help make full use of CYC and reduce the environmental impacts of using multiple pesticides.

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“…Both FAS products and fatty acids taken up from the diet are further elongated into very long chain fatty acids (VLCFA) [122]. Elongases of very long chain fatty acid (ELOVL) are enzymes that are essential for the biosynthesis of VLCFAs and elongation of long-chain fatty acids [129]. ELOVLs, which are conserved from microorganisms to mammals, play key roles in various biological processes [130].…”

Section: Resultsmentioning

confidence: 99%

“…mori and R . prolixus [122, 129, 131]. In the present study, we obtained a total of six unigenes encoding ELOVL proteins from the P .…”

Section: Resultsmentioning

confidence: 99%

De novo transcriptome analysis and identification of genes associated with immunity, detoxification and energy metabolism from the fat body of the tephritid gall fly, Procecidochares utilis

Gao

Lan

et al. 2019

PLoS ONE

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The fat body, a multifunctional organ analogous to the liver and fat tissue of vertebrates, plays an important role in insect life cycles. The fat body is involved in protein storage, energy metabolism, elimination of xenobiotics, and production of immunity regulator-like proteins. However, the molecular mechanism of the fat body’s physiological functions in the tephritid stem gall-forming fly, Procecidochares utilis, are still unknown. In this study, we performed transcriptome analysis of the fat body of P. utilis using Illumina sequencing technology. In total, 3.71 G of clean reads were obtained and assembled into 30,559 unigenes, with an average length of 539 bp. Among those unigenes, 21,439 (70.16%) were annotated based on sequence similarity to proteins in NCBI’s non-redundant protein sequence database (Nr). Sequences were also compared to NCBI’s non-redundant nucleotide sequence database (Nt), a manually curated and reviewed protein sequence database (SwissProt), and KEGG and gene ontology annotations were applied to better understand the functions of these unigenes. A comparative analysis was performed to identify unigenes related to detoxification, immunity and energy metabolism. Many unigenes involved in detoxification were identified, including 50 unigenes of putative cytochrome P450s (P450s), 18 of glutathione S-transferases (GSTs), 35 of carboxylesterases (CarEs) and 26 of ATP-binding cassette (ABC) transporters. Many unigenes related to immunity were identified, including 17 putative serpin genes, five peptidoglycan recognition proteins (PGRPs) and four lysozyme genes. In addition, unigenes potentially involved in energy metabolism, including 18 lipase genes, five fatty acid synthase (FAS) genes and six elongases of very long chain fatty acid (ELOVL) genes, were identified. This transcriptome improves our genetic understanding of P. utilis and the identification of a numerous transcripts in the fat body of P. utilis offer a series of valuable molecular resources for future studies on the functions of these genes.

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Genome-wide identification and analysis of elongase of very long chain fatty acid genes in the silkworm, Bombyx mori

Cited by 18 publications

References 50 publications

Haemolymph metabolomic differences in silkworms (Bombyx mori L.) under mulberry leaf and two artificial diet rearing methods

Haemolymph metabolomic differences in silkworms (Bombyx mori L.) under mulberry leaf and two artificial diet rearing methods

Molecular Insights into the Insensitivity of Lepidopteran Pests to Cycloxaprid

De novo transcriptome analysis and identification of genes associated with immunity, detoxification and energy metabolism from the fat body of the tephritid gall fly, Procecidochares utilis

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