Genotoxicity induced by iodine-131 in human cultured lymphocytes

Hosseinimehr, Seyed Jalal; Shafaghati, Nayereh; Hedayati, Monireh

doi:10.2478/intox-2013-0013

Cited by 11 publications

(10 citation statements)

References 13 publications

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“…It concentrates in thyroid cells and kills tumor cells, yet it has several side effects such as sialadenitis, gastrointestinal symptoms, xerostomia, temporary bone-marrow suppression and neoplasia (Bushnell et al , 1992 , Noaparast et al , 2013 ). 131 I may also induce genetic damage and chromosomal instability in normal cells that may result in secondary malignancies (Baugnet-Mahieu et al , 1994 , Watanabe et al , 2004 , Hosseinimehr et al , 2013 ). The cytotoxic effect of 131 I is mainly related to beta particles.…”

Section: Introductionmentioning

confidence: 99%

Radiosensitive effect of curcumin on thyroid cancer cell death induced by radioiodine-131

Hosseinimehr

Hosseini

2014

Interdisciplinary Toxicology

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Curcumin is a natural product widely consumed by humans. It has many biological properties. In this study, we investigated the radiosensitive effect of curcumin on thyroid cancer cells against cellular toxicity induced by 131-I. Human thyroid cancer and human non-malignant fibroblast cells (HFFF2) were treated with 131-I and/or curcumin at different concentrations (5, 10 and 25 µg/ml) for 48 h. The cell proliferation was measured by determination of the surviving cells by using MTT assay. Our results showed that curcumin increased the killing effect of 131-I on thyroid cancer cells, while it exerted no toxicity on HFFF2 cells. This result shows a promising effect of curcumin on the enhancement of therapeutic effects of 131-I in patients.

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Section: Introductionmentioning

confidence: 99%

Radiosensitive effect of curcumin on thyroid cancer cell death induced by radioiodine-131

Hosseinimehr

Hosseini

2014

Interdisciplinary Toxicology

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“…They reported that 10 and 100 microcurie doses of I-131 increased the number of micronuclei 8.5 times more than the control group. [ 13 ] In another study, Eberlein et al . evaluated the DSBs caused by I-131 and Lu-137 and showed a linear relationship between the absorbed radiation dose and the number of DSBs.…”

Section: Discussionmentioning

confidence: 99%

“…[ 8 9 10 ] Furthermore, it may induce chromosomal instability and genetic damage in normal cells that may cause in secondary cancers. [ 11 12 13 ] The cellular damage resulting from I-131 can be through direct ionization or creation of reactive oxygen spaces. [ 14 ] One of the vital aims during the interaction between ionization radiations and the cells is the cell DNA in which can cause a range of damages such as single-strand break and double-strand break (DSB).…”

Section: Introductionmentioning

confidence: 99%

The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131

et al. 2018

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Background:Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes.Materials and Methods:First, 6-mL blood samples were taken from each of the five volunteers. After 1 h of preincubation with the antioxidants, a total of 20 μCi I-131/2 mL (blood + NaCl) was added to each sample, and then, the samples were reincubated for 1 h. Lymphocytes were separated and the mean DSB levels were measured for each sample through γ-H2AX assay to evaluate the effects of antioxidants.Results:After 1-h incubation with I-131, the DSBs increased by 102.9% compared to the control group (0.343 vs. 0.169 DSB/cell; P = 0.00). Furthermore, compared to the control + I-131 group, curcumin and trehalose reduced the DSBs by 42% and 38%, respectively. There was a significant decrement (P = 0.00) in the levels of DSBs of the curcumin + I-131 and trehalose + I-131 subgroups compared to the control + I-131 subgroup. Furthermore, there was no significant relationship between the radioprotective effect of curcumin and trehalose (P = 0.95).Conclusion:The use of curcumin and trehalose as antioxidant can reduce the numbers of DSBs caused by I-131. Meanwhile, the radioprotective effect of curcumin was more than trehalose.

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“…By the by, toxic effect on immune pathways could be elucidated (Guzik et al ., 2001). Genotoxicity testing of 131-radioiodine a drug used for treatment of patients with thyroid diseases with cultured human lymphocytes was recently described (Hosseinimehr et al ., 2013 ), as a work in predictive toxicology.…”

Section: Discussionmentioning

confidence: 99%

Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes

Patnaik

Padhy

2015

Interdisciplinary Toxicology

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This work describes that cytotoxicity of lead chloride and lead acetate to in vitro cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl2. Lethal concentration25 (LC25) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC50 values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC100 values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC25 values were almost in the range: 295.12 to 371.53 mg/L; LC50 values were in the range: 501.18 to 588.84 mg/L; LC100 value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC100 values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes.

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Genotoxicity induced by iodine-131 in human cultured lymphocytes

Cited by 11 publications

References 13 publications

Radiosensitive effect of curcumin on thyroid cancer cell death induced by radioiodine-131

Radiosensitive effect of curcumin on thyroid cancer cell death induced by radioiodine-131

The Radioprotective Effects of Curcumin and Trehalose Against Genetic Damage Caused By I-131

Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes

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