GHRH|HaeIII Gene Polymorphism in Dairy and Beef Cattle at National Livestock Breeding Centers

Rini, A. O.; Sumantri, Cece; Anggraini, Anita Dwi

doi:10.5398/medpet.2013.36.3.185

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…The GHRH|HaeIII locus or the GHRH g.4474 C>A SNP re-sulted in three genotypes, i.e., AA genotype by 3 bands (312, 94, and 45 bp), CC genotype by 4 bands (194, 118, 94, and 45 bp), and AC genotype by 5 bands (312, 194, 118, 94, and 45 bp). These three genotypes were consistent to the results reported by previous study (Rini et al, 2013).…”

Section: Ghrh G4474 C>a Snp Polymorphismsupporting

confidence: 93%

“…The same results were reported on this genetic polymorphism of HF cattle from the two AI centers and an ET Center providing a very rare of the AA (4.5%) cattle than the AC cattle (37.1%) and CC cattle (58.4%); likewise generating a very low A allele (22.5%) rather than C allele (77.5%) (Rini et al, 2013). Some previous studies also confirmed the same results for the lowest AA animals compared to the AB (AC) and BB (CC) animals, such as in Polish Holstein i.e., 7.8% vs. 33.9% and 58.3%; abd Jersey i.e., 3.2% vs. 42.7% and 54.1% (Szatkowska et al, 2009).…”

Section: Ghrh G4474 C>a Snp Polymorphismsupporting

confidence: 81%

“…Amplified DNA product of the GHRH gene produced the base fragment by 451 bp, located in exon 2, intron 2, and exon 3 of this gene. This fragment fitted to the genome library (GenBank: AF242855) as previously reported by Rini et al (2013). The HaeIII restriction enzyme recognized the cutting base fragment at the nucleotide sites of 4472, 4666, and 4760 or at the base positions of 118, 312, and 406 of the PCR products.…”

Section: Ghrh G4474 C>a Snp Polymorphismmentioning

confidence: 92%

“…Specific restriction enzymes used to cut each of GH, GHRH and Pit-1 genes were successively MspI, HaeIII, and HinfI. The MspI enzyme recognizes base sites of C*CGG or GH g.1456_1457 InsT (Dybus, 2002), the HaeIII enzyme recognizes base sites of GG*CC or GHRH g.4474 C>A SNP (Rini et al, 2013), and HinfI enzyme recognizes base sites of G*ANTC or Pit-1 g.244 G>A SNP (Dybus et al, 2004).…”

Section: Amplificationmentioning

confidence: 99%

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Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

Anggraeni¹,

Sumantri

Saputra³

et al. 2020

Trop. Anim. Sci. J.

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Lactation traits are controlled by many genes, among others, potentially by growth genes. This research was conducted to study genetic polymorphisms of GH, GHRH, and Pit-1 genes and associations of GH (g.1456_1457 InsT), GHRH (g.4474 C>A), and Pit-1 (g.244 G>A) genotypes with milk yield and quality in Holstein Friesian (HF) cattle. Genotyping was conducted for HF dairy cows raised by small farmers from North Lembang (SF-NL) (98 heads) and South Lembang (SF-SL) (95 heads), and also from Cikole Dairy Cattle Station (CDCS) (82 heads) in Lembang, West Java, Indonesia. Progeny tested of HF bulls (17 heads) from LAIC (Lembang Artificial Insemination Center) in West Java and from SAIC (Singosari AIC) (32 heads) in East Java were also genotyped. Effects of genotypes on test day milk yield, fat content, and SNF content were analyzed by General Linear Models. The GHRH g.4474 C>A SNP and Pit-1 g.244 G>A SNP generated high frequencies of C allele to A allele, while the two allelic frequencies of the GH g.1456_1457 InsT varied. Compared to the AA and AC genotypes, the CC genotype of the GH gene resulted higher test day milk yield (p<0.01), fat content (p<0.05), and SNF content (p<0.05). Further the CC genotype of the GHRH gene yielded higher milk yield (p<0.05), while the GG genotype of the Pit-1 gene resulted higher fat content (p<0.05). Therefore the GH g.1456_1457 InsT, GHRH g.4474 C>A SNP, and Pit-1 g.244 G>A SNP are potential to be used as molecular markers for selection on milk yield and quality in domestic HF cattle.

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Section: Ghrh G4474 C>a Snp Polymorphismsupporting

confidence: 93%

Section: Ghrh G4474 C>a Snp Polymorphismsupporting

confidence: 81%

Section: Ghrh G4474 C>a Snp Polymorphismmentioning

confidence: 92%

Section: Amplificationmentioning

confidence: 99%

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Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

Anggraeni¹,

Sumantri

Saputra³

et al. 2020

Trop. Anim. Sci. J.

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“…Similar frequencies of rare GHRH A allele, 0.2480 and 0.2400 were reported by Dybus and Grzestak (2006) and Klauzinska et al (2004), respectively. Further, frequencies for the GHRH A and GHRH B allele vary considerably between breeds, GHRH A allele was found to be lowest in cattle breeds such as the Hereford (Moody et al, 1995), Holstein (Kmiec et al, 2007;Szatkowska et al, 2009;Rini et al, 2013), Jersey (Szatkowska et al, 2009), Simmental and Limousin (Rini et al, 2013;Dybus et al, 2003). In contrast to those breeds, GHRH A allele frequency was found to highest in Angus cattle (Moody et al,1995;Rini et al, 2013), whereas the GHRH B allele was found with a maximum frequency in Brahman breed which originates from Bos indicus (Rini et al, 2013).…”

Section: Restriction Patterns With Haeiii Endonucleasementioning

confidence: 99%

Effect of Growth Hormone-releasing Hormone Gene Polymorphism (GHRH/HAEIII) on First Lactation Milk Yield and Milk Composition Traits in Sahiwal Cattle

Singh¹,

Arya²,

Singh³

et al. 2021

IJAR

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Background: Growth hormone-releasing hormone (GHRH), also known as somatoliberin is a releasing hormone of growth hormone (GH). GHRH is a peptide hormone found mainly in the hypothalamus. The gene is significantly associated with increase milk yield in cattle. The objective of this study was to identify the polymorphism in the GHRH gene (GHRH/HaeIII) and to evaluate the relationship between the polymorphism with the breeding values for first lactation milk production and composition traits of Sahiwal cattle reared using PCR-RFLP. Methods: A total of 130 animals were included in the study. PCR-RFLP analysis of each 297bp PCR product was carried out using HaeIII restriction enzyme. The single trait animal model was considered for the estimation of breeding values using WOMBAT software. To study the effect of genotype on the individual trait, PROC GLM (SAS 4.3) was used.Result: PCR-RFLP analysis of GHRH gene revealed three genotypes with genotypic frequencies as; 0.05 (AA), 0.42 (AB) and 0.53 (BB). The allelic frequencies for A and B allele were 0.26 and 0.74, respectively. Sahiwal cows with AA genotype have significantly higher (p less than 0.017) fat percentage in milk while the cows with BB genotype; while the BB genotype cows characterized by significantly higher (p less than 0.025) first lactation 305 day milk yield. Among the three genotypes, BB was most abundant while AA genotype was rarest in the screened Sahiwal samples. The identified potential genetic marker could be used for the development of Marker Assisted Selection (MAS) strategy for higher milk yield and milk composition traits in Sahiwal cattle.

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Integrating genome-wide association study and pathway analysis reveals physiological aspects affecting heifer early calving defined at different ages in Nelore cattle

et al. 2022

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GHRH|HaeIII Gene Polymorphism in Dairy and Beef Cattle at National Livestock Breeding Centers

Cited by 4 publications

References 17 publications

Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

Effect of Growth Hormone-releasing Hormone Gene Polymorphism (GHRH/HAEIII) on First Lactation Milk Yield and Milk Composition Traits in Sahiwal Cattle

Integrating genome-wide association study and pathway analysis reveals physiological aspects affecting heifer early calving defined at different ages in Nelore cattle

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