Granulocyte markers in induced sputum in patients with respiratory disorders and healthy persons obtained by two sputum-processing methods

Metso, T.; Rytil, P.; Peterson, Christopher W.; Haahtela, Tari

doi:10.1053/rmed.2000.0970

Cited by 44 publications

(48 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This inflammation may respond poorly to conventional treatments [21] (I. Helenius, Helsinki University Central Hospital, unpublished results), but appears to be partly reversible after stopping intensive training [20]. The sputum differential cell count of the athletes is also very similar to that observed in patients with lower respiratory tract infection [22].…”

Section: Validity Of the Datamentioning

confidence: 74%

Airway inflammation, bronchial hyperresponsiveness and asthma in elite ice hockey players

Lumme

Haahtela²,

Ounap³

et al. 2003

Eur Respir J

Self Cite

View full text Add to dashboard Cite

Airway inflammation, bronchial hyperresponsiveness and asthma in elite ice hockey players. A. Lumme, T. Haahtela, J. Ö unap, P. Rytilä, Y. Obase, M. Helenius, V. Remes, I. Helenius. #ERS Journals Ltd 2003. ABSTRACT: There is little information of lower respiratory symptoms, bronchial hyperresponsiveness and airway inflammation in elite ice hockey players.A total of 88 highly trained ice hockey players and 47 control subjects were studied. All the subjects were subjected to skin-prick tests, resting spirometry examinations and histamine-challenge tests. Adequate induced sputum samples were obtained from 68 of the ice hockey players and from 18 symptom-free control subjects on a separate day.Bronchial hyperresponsiveness in a histamine-challenge test was found in 21 (24%) of the athletes and in five (11%) of the controls. Current asthma (current asthmatic symptoms and increased bronchial responsiveness) was observed in 13 (15%) of the athletes and in one (2%) of the control subjects. Total asthma (current asthma or previously physician-diagnosed asthma) occurred in 19 (22%) of the athletes and in two (4%) of the controls. Atopy, according to skin-prick tests, was observed in 51 (58%) of the athletes and 17 (36%) of the control subjects. The differential cell counts of eosinophils (2.6 versus 0.2%) and neutrophils (80.9 versus 29.9%) in the sputum samples of the ice hockey players were significantly higher than in those of the control subjects.Asthma is common in elite ice hockey players and they show signs of a mixed type of neutrophilic and eosinophilic airway inflammation. Inhalation of cold air associated with exposure to indoor pollutants during intensive training is a possible causative factor.

show abstract

Section: Validity Of the Datamentioning

confidence: 74%

Airway inflammation, bronchial hyperresponsiveness and asthma in elite ice hockey players

Lumme

Haahtela²,

Ounap³

et al. 2003

Eur Respir J

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although, the levels of iNOS have been found to be elevated in the induced sputum of asthmatics (Sugiura et al 2003), variable levels of iNOS have been reported in COPD patients (Ichinose et al 2000;Rytila et al 2006). EPO is elevated in asthma while MPO, which is expressed in neutrophils and monocytes (Kim et al 2001), is elevated in COPD with the levels being higher in COPD than in asthma (Metso et al 2001). A rather similar elevation in the number of MPO-positive cells is seen in the induced sputum of chronic nonsymptomatic smokers and chronic symptomatic smokers with normal lung function, who are considered to be at risk of developing COPD .…”

Section: Oxidant-generating Enzymesmentioning

confidence: 99%

Airway biomarkers of the oxidant burden in asthma and chronic obstructive pulmonary disease: Current and future perspectives

Louhelainen

Myllärniemi²,

Rahman³

et al. 2008

COPD

View full text Add to dashboard Cite

Abstract:The pathogenesis of asthma and chronic obstructive pulmonary disease (COPD) has been claimed to be attributable to increased systemic and local oxidative stress. Detection of the oxidant burden and evaluation of their progression and phenotypes by oxidant biomarkers have proved challenging and diffi cult. A large number of asthmatics are cigarette smokers and smoke itself contains oxidants complicating further the use of oxidant biomarkers. One of the most widely used oxidant markers in asthma is exhaled nitric oxide (NO), which plays an important role in the pathogenesis of asthma and disease monitoring. Another oxidant marker that has been widely investigated in COPD is 8-isoprostane, but it is probably not capable of differentiating asthma from COPD, or even sensitive in the early assessment of these diseases. None of the current biomarkers have been shown to be better than exhaled NO in asthma. There is a need to identify new biomarkers for obstructive airway diseases, especially their differential diagnosis. A comprehensive evaluation of oxidant markers and their combinations will be presented in this review. In brief, it seems that additional analyses utilizing powerful tools such as genomics, metabolomics, lipidomics, and proteomics will be required to improve the specifi city and sensitivity of the next generation of biomarkers. Keywords: sputum, condensate, smoking, nitric oxide, 8-isoprostane, biomarker Asthma and chronic obstructive pulmonary disease (COPD) are diseases with different physiological and anatomical features. However, a signifi cant number of patients suffering from these diseases exhibit symptoms and signs which are associated with both conditions. Markers of the elevated oxidant burden have been detected in both of these disorders. This degree of overlap between asthma and COPD has proved to be especially important in smoking asthmatics, patients who have often been excluded from asthma trials and investigations. Oxidant markers are under intensive investigation as ways of assuring the early detection and monitoring of asthma and COPD, for the differentiation of the disease from the condition of "healthy smokers", and also for the evaluation of the COPD phenotypes and their progression. This review will critically review potential markers that refl ect oxidative stress in the airways of patients with asthma and COPD in exhaled air, exhaled breath condensate (EBC) and induced sputum. In addition, markers in bronchoalveolar lavage (BAL) will also be briefl y surveyed, even though the technique, ie, fi breoptic bronchoscopy is an invasive technique used in the investigation of lung infl ammation and the evaluation of the oxidant burden. In this review, we will also discuss the pros and cons of various noninvasive biological samples for assessing the oxidant burden in the airways, and then speculate on the possibilities of using the existing and new oxidant markers,

show abstract

“…Flow-volume curves were generated, FeNO and airway hyperresponsiveness to acetylcholine were measured, and sputum was induced and processed in accordance with previously reported procedures [7,8,10,11].…”

Section: Other Examinationsmentioning

confidence: 99%

Lung Sound Analysis Is Useful for Monitoring Therapy in Patients With Bronchial Asthma

Shimoda¹,

Obase²,

Nagasaka³

et al. 2017

J Investig Allergol Clin Immunol

View full text Add to dashboard Cite

Background: Lung sound analysis (LSA) has been reported to be useful for predicting airway obstruction and inflammation in patients with bronchial asthma. Objectives: We examined whether the exhalation-to-inhalation sound pressure ratio in the middle frequency range (200-400 Hz) (E/I MF) is useful for monitoring therapy in patients with asthma. Methods: The study population comprised 84 patients with mild to moderate asthma whose LSA data were available before and after 1 year of daily treatment with (budesonide 800 μg). We analyzed whether the E/I MF before and after treatment was associated with the fractional exhaled nitric oxide (FeNO) level, sputum eosinophil percentage, respiratory function, and airway hyperresponsiveness. Results: Prior to treatment with budesonide, the E/I MF was significantly correlated with respiratory function, airway hyperresponsiveness, FeNO, and sputum eosinophil percentage. The cutoff values for the E/I MF to detect the abnormalities of respiratory function, FeNO, and sputum eosinophil percentage were 0.367, 0.358, and 0.363, respectively. With respect to the reference value, the E/I MF improved significantly in patients whose respiratory function and FeNO benefited from therapy with budesonide compared with patients whose respiratory function did not benefit from budesonide (odds ratios of 6.39 and 4.78, respectively). According to the multivariate analysis, patients whose E/I MF did not improve had a longer history of smoking (P=.038), poorer posttreatment respiratory function (P=.028), and higher posttreatment FeNO (P=.0095). Conclusion: Similar to respiratory function and FeNO, E/I MF based on LSA is a useful indicator for monitoring the efficacy of therapy in asthmatic patients.

show abstract

Granulocyte markers in induced sputum in patients with respiratory disorders and healthy persons obtained by two sputum-processing methods

Cited by 44 publications

References 31 publications

Airway inflammation, bronchial hyperresponsiveness and asthma in elite ice hockey players

Airway inflammation, bronchial hyperresponsiveness and asthma in elite ice hockey players

Airway biomarkers of the oxidant burden in asthma and chronic obstructive pulmonary disease: Current and future perspectives

Lung Sound Analysis Is Useful for Monitoring Therapy in Patients With Bronchial Asthma

Contact Info

Product

Resources

About