Green synthesis of silver nanoparticle by cauliflower extract: characterisation and antibacterial activity against storage

Abstract

Green synthesis of silver nanoparticles (AgNPs) was accomplished using different volumes of cauliflower extract and 0.001 M silver nitrate solution at 80°C for 15 min. A brownish-red solution of AgNPs formed was tested by ultraviolet-visible absorption spectroscopy, Fourier-transform infrared (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). Surface plasmon resonance of AgNPs appeared at 416 nm. Also, the kinetic of AgNPs formation was studied and follows a sigmoidal pattern. Storing time was studied for the freshly prepared AgNPs after 60 days. FTIR analysis shows the adsorption of active components on AgNPs surface, and these components are responsible for reduction besides working as a stabiliser like a capping agent, also FTIR analysis of AgNPs after storage showed no change in peaks location. The SEM exhibited a globular shape of AgNPs, and the particle size ranged from 25 to 100 nm, while the XRD particle size calculation was 25 nm with cubic phase lattice.The antibacterial activity was tested against Gram-positive and-negative bacteria showed an inhibition zone of 16-27 mm and the antibacterial activity tested for the same bacteria after storage for about 10 months showed an inhibition zone of 6-10 mm.

Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization

“…Furthermore, silver nanoparticles were produced at various AgNO3 concentrations with ethanolic extract of E. americana bulb, as evidenced by the plasmon resonance band found at 410 nm within the UV spectra (corresponding to Ea-AgNPs); AgNO3 had an absorbance peak at 300 nm and the ethanolic extract of E. americana bulb had a peak at between 500-600 nm. These findings are comparable with those of Pandian et al (2015), stating that Ea-AgNPs shows a characteristic peak absorbance wavelength between 400-500 nm (Figure 2). Previous research has also noted that silver nanoparticle absorb light between 400 and 500 nm due to their surface plasmon resonance (Prathna et al 2011).…”

“…Other research on the biosynthesis of AgNPs using various plant extracts are as follows: Oscillatoria sp. extracts (Adebayo-Tayo et al 2019), cauliflower extract (Oda et al 2019), and the bulb of Myrmecodia sp. (Nugroho et al 2021).…”

Biosynthesis of silver nanoparticle by green wheat spike extract characterization and antibacterial activity

“…This color interestingly belongs to common formation of silver nanoparticles, which indicate as a signature as reported in literatures [25]. The active component of green wheat is responsible of bioreduction and silver nanoparticle formation without any precipitate [14,26]. The process is monitored by uv-vis spectroscopy and the spectra in the range of 200-800 nm as in figure 1, where the absorption at maximum value at 402 nm.…”

“…In addition, silver nanoparticles have low toxic effect on human cell and a good thermal stability and this encourage being useful in drugs industries [7,8].Green synthesis of silver particles can be adapted by biological synthesis seeking to high consideration of safe nanomaterial by using eco -friendly represented by plant extract in comparison to other chemical and physical routes. Less feasibility and hazardous material by chemical method and high cost of physical methods in synthesis of silver nanoparticle; commonly used like chemical reductio n and ion sputtering, it necessary to overcome these method by alternative green synthesis route [9][10][11].The natural products used in green synthesis are biologically non-toxic product and safe for using medical and pharmaceutical industry, also using green synthesis is easily, one step and one pot reaction, ecofriendly and cost effective [12].The procedure of silver nanoparticle in green synthesis depend on using plant extract, chitosan and microorganism work as reductant of soluble ion and after conversion work as capping agent to be more stable and shelf life [13].Plant extracts were widely used for synthesis that proved their activity in reduction with high stability like previous study are the synthesis by cauliflower extractwith long shelf life [14],ficuscordata leaves extract [15], olive oil in organic solvent [16],AcalyphaindicaGelidium [17], amansii [18], and mangosteen [19].…”

Selection of suitable reference genes for real-time qPCR gene expression in cauliflower under abiotic stress and methyl jasmonate treatment

“…Medical research has revealed that a diet rich in cauliflower can lower the risk of cancer (Bergès et al 2018, Kalisz et al 2018. Cauliflower contains glucosinolates, a class of secondary plant metabolites; their hydrolyzed products have anti-carcinogenic properties (Oda et al 2019). The findings of multiple studies have indicated that environmental stresses can increase the accumulation of glucosinolates (Jousef et al 2018, Oda et al 2019, but the glucosinolate content in most cauliflower plants is very low.…”