Group-specific human granulocyte antigens on a chronic myelogenous leukemia cell line with a Philadelphia chromosome marker

Abstract

Group-specific human granulocyte antigens are serologically detectable with granulocytotoxic-positive human alloantisera on a cell line, K562, of chronic myelogenous leukemia origin which bears a Philadelphia chromosomal marker. The same cell line lacks serologically detectable HLA, B2 microglobulin, and B-lymphocyte antigens. Granulocyte antigens are important cell markers for cell lines of suspected myeloid lineage.

Cloning and structural characterization of a human non-erythroid band 3-like protein.

“…Although the K562(S) cell line has been described as eryftiroidlike, these cells express granulocytic antigens (Drew et al, 1977;Marie et al, 1981) as well as erythroid markers (Gahmberg et al, 1979;Marie et al, 1981). This cell line appears to undergo only limited erythroid differentiation upon butyrate induction resulting in the production of small amounts of fetal and embryonic hemoglobins (Cioe et al, 1981).…”

A transcriptional enhancer with specificity for erythroid cells is located in the long terminal repeat of the Friend murine leukemia virus.

“…It was originally thought to represent a primitive granulocyte cell. This view was based mainly on morphological examination, the absence of lymphoid surface makers and the presence of granulocyte antigens (Klein et al, 1976;Lozzio et al, 1976;Drew et al, 1977). However, these studies did not prove that K562 cells were myeloid precursor cells, and later studies demonstrated biochemically that K562 cells do show an erythroid phenotype (Anderson et al, 1979;Rutherford et al, 1979, 198 1a;Hoffman et al, 1981).…”

Cloning and Characterization of Primary Alloreactive Human T‐Lymphocytes

“…These results indicated that clones IV-6IA, IV-61B and IV-22, derived from 24 h alloactivated cells during the priming KC DS,,. were capable of nonspecific cytotoxic activity against the human leukemic cell line K562, which expresses no detectable HLA-A,B,C or DR antigens (Drew et al 1977) and is highly susceptible to lysis by NK cells. Alloantigen-specific cytotoxic cells from bulk MLC similarly displayed strong killing of K562 target cells.…”