Growth of14C-labelled starch granules in potato tubers as revealed by autoradiographs

Badenhuizen, N. P.; Dutton, R.W.

doi:10.1007/bf01252442

Cited by 58 publications

(22 citation statements)

References 5 publications

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“…The location of most of the branching enzyme on the surface of the starch granule implies that the actual branching takes place predominantly at that surface and not throughout by Badenhuizen and Dutton (Badenhuizen and Dutton, 1956), although our interpretation differs from the one they give in their report. They argue that very likely a starch-like substance is formed in the amyloplast that starts to crystallize from solution when a certain concentration has been reached.…”

Section: Discussioncontrasting

confidence: 84%

Localization of Branching Enzyme in Potato Tuber Cells with the Use of Immunoelectron Microscopy

Kram¹,

Oostergetel²,

Bruggen³

1993

Plant Physiol.

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Potato branching enzyme, a key enzyme in the biosynthesis of starch, was localized in amyloplasts in starch-storage cells of potato (Solanum tuberosum 1.) with the use of immunogold electron microscopy. Branching enzyme was found in the amyloplast stroma, concentrated at the interface of the stroma and the surface of the starch granule. ADP-glucose pyrophosphorylase, a key regulatory enzyme in starch synthesis, was localized for comparison to exclude possible artifacts. ADP-glucose pyrophosphorylase, in contrast with branching enzyme, proved to be evenly distributed throughout the stroma. Branching enzyme also appears to be present in a membrane-bounded inclusion body in the stroma, whereas ADP-glucose pyrophosphorylase is not. The presence of branching enzyme predominantly at the surface of the starch granule indicates that branching takes place at that surface and not throughout the amyloplast stroma.

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Section: Discussioncontrasting

confidence: 84%

Localization of Branching Enzyme in Potato Tuber Cells with the Use of Immunoelectron Microscopy

Kram¹,

Oostergetel²,

Bruggen³

1993

Plant Physiol.

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“…In this study, the localization of BE leads to a conclusion that the actual branching for synthesis of amylopectin would take place predominantly throughout the surface of each starch granule, but not throughout the stroma. This result is consistent with the presumption by Badenhuizen and Dutton ( 1956) that amylopectin is synthesized at the surface of starch granules in accord with growth of the granules. In addition, the label for BE was also concentrated around small starch granules that were present in parts of the periphery in amyloplasts.…”

Section: Discussionsupporting

confidence: 81%

Structural and Immunocytochemical Characterization of the Synthesis and Accumulation of Starch in Sweet Potato (Ipomoea batatasLam.) Tuberous Root

Kawasaki

Matsuda

Nakamura

et al. 2002

Plant Production Science

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“…3). Based on the growth of starch granules, which occurs by apposition [4], this might indicate that once the transition from amylose-containing to amylose-free starch has been made, the synthesis of amylose-free starch will continue until the development of the granules is completed. Due to the increase of the red-staining amylose-free zone, the ratio between the size of the blue-staining core and total granule size, as well as the average amylose content and GBSS activity in isolated starch will decrease, thus resulting in an increase of the antisense effect.…”

Section: Antisense Inhibition During Tuber Growthmentioning

confidence: 99%

Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth

et al. 1994

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Transgenic plants of a tetraploid potato cultivar were obtained in which the amylose content of tuber starch was reduced via antisense RNA-mediated inhibition of the expression of the gene encoding granule-bound starch synthase (GBSS). GBSS is one of the key enzymes in the biosynthesis of starch and catalyses the formation of amylose. The antisense GBSS genes, based on the full-length GBSS cDNA driven by the 35S CaMV promoter or the potato GBSS promoter, were introduced into the potato genome by Agrobacterium tumefaciens-mediated transformation. Expression of each of these genes resulted in the complete inhibition of GBSS gene expression, and thus in the production of amylose-free tuber starch, in mature field-grown plants originating from rooted in vitro plantlets of 4 out of 66 transgenic clones. Clones in which the GBSS gene expression was incompletely inhibited showed an increase of the extent of inhibition during tuber growth. This is likely to be due to the increase of starch granule size during tuber growth and the specific distribution pattern of starch components in granules of clones with reduced GBSS activity. Expression of the antisense GBSS gene from the GBSS promoter resulted in a higher stability of inhibition in tubers of field-grown plants as compared to expression from the 35S CaMV promoter. Field analysis of the transgenic clones indicated that inhibition of GBSS gene expression could be achieved without significantly affecting the starch and sugar content of transgenic tubers, the expression level of other genes involved in starch and tuber metabolism and agronomic characteristics such as yield and dry matter content.

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Growth of14C-labelled starch granules in potato tubers as revealed by autoradiographs

Cited by 58 publications

References 5 publications

Localization of Branching Enzyme in Potato Tuber Cells with the Use of Immunoelectron Microscopy

Localization of Branching Enzyme in Potato Tuber Cells with the Use of Immunoelectron Microscopy

Structural and Immunocytochemical Characterization of the Synthesis and Accumulation of Starch in Sweet Potato (Ipomoea batatasLam.) Tuberous Root

Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth

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