Growth-Plate Chondrocyte Cultures for Reimplantation Into Growth-Plate Defects in Sheep

Hansen, A. L.; Foster, Bruce K.; Gibson, G. Edward; Binns, Gregory F.; Wiebkin, Ole W.; Hopwood, John J.

doi:10.1097/00003086-199007000-00040

Cited by 34 publications

(4 citation statements)

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“…The implantation of cartilage and cultured chondrocytes has also been used in animal experiments to prevent recurrence of the bony bridges [1,2,8,9,10,13,16,22,24,33,45]. Cartilage implants were found to be better than fat grafts for preventing shortening of the limb and angular deformity because of an inhibitory effect on bone [24,33].…”

Section: Introductionmentioning

confidence: 99%

The implantation of cartilaginous and periosteal tissue into growth plate defects

Wirth

Byers

Byard

et al. 1994

International Orthopaedics

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This experimental study reports the results of implantation of cartilaginous and periosteal tissues into growth plate defects in the tibiae of sheep. When no material was used, the defect rapidly filled with marrow-like tissue. When cartilage from the margin of the secondary centre of ossification was implanted, endochondral ossification continued and no shortening or deformity resulted. Implantation of periosteum with or without reconstructed peripheral tissues resulted in the formation of a bony bridge which led to a 32% inhibition of longitudinal growth and a 12 degrees varus deformity in the absence of peripheral connective tissues. After reconstruction with these tissues, the inhibition of longitudinal growth was 47% with a 28 degrees varus deformity. The chondroprogenitor cells in the implanted tissues cannot change phenotypic expression. Periosteum has a strong potential for bone formation after it has been implanted.

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Section: Introductionmentioning

confidence: 99%

The implantation of cartilaginous and periosteal tissue into growth plate defects

Wirth

Byers

Byard

et al. 1994

International Orthopaedics

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“…With the development of cell-based strategies, there have been reports on the use of cultured chondrocytes to repair physeal defects. [30][31][32] Our previous study showed that chondrocytes transferred with agarose into large physeal defect (50% of physis) could prevent the growth arrest with angular deformity. 31 However, transfer of cultured chondrocytes from the iliac apophysis and articular cartilage involved the sacrifice of a significant amount of iliac apophysis and articular cartilage.…”

Section: Discussionmentioning

confidence: 99%

11th Yahya Cohen Memorial Lecture – An In vivo Comparative Study of the Ability of Derived Mesenchymal Stem Cells in the Treatment of Partial Growth Arrest

Hui

Ouyang

et al. 2009

Ann Acad Med Singap

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Few in vivo studies had previously been attempted in reaffirming the in vitro data in current literature. This study evaluated the ability of mesenchymal stem cells (MSCs) isolated from bone marrow, periosteum and fat to treat partial growth arrest in immature New Zealand white (NZW) rabbits. A physeal arrest model in an immature rabbit was created. The bony bridge was excised 3 weeks later, and MSCs from various sources were transferred into the physeal defect of different rabbits. Group I consisted of bone marrow-derived MSCs, Group II: periosteum- derived MSCs, Group III: fat-derived MSCs. Contra-lateral tibiae, without undergoing opera- tion, served as self-control. The animals were subsequently sacrificed, with radiological and histological analyses performed. All MSCs demonstrated chondrogenic and osteogenic differen- tiation potentials in vitro. In correction of varus angulation groups I and II exhibited superior results when compared to group III (P <0.05). The length discrepancies between operated and normal tibiae in groups I, II and III were significantly corrected when compared to the control group (P <0.01). In conclusion, bone marrow and periosteum derived stem cells provided better correction of physeal arrest in rabbits. The source of MSCs itself could influence the success in the treatment of growth arrest. Key words: Bone marrow, Fats, Periosteum

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“…With the development of cell-based strategies, there have been reports on the use of cultured chondrocytes to repair physeal defects. [30][31][32] Our previous study showed that chondrocytes transferred with agarose into a large physeal defect (50% of physis) could prevent growth arrest with angular deformity. 31 However, transfer of cultured chondrocytes from the iliac apophysis and articular car-TREATMENT OF PARTIAL GROWTH ARREST WITH MSCs tilage involved sacrifice of a significant amount of iliac apophysis and articular cartilage.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of the Ability of Mesenchymal Stem Cells Derived from Bone Marrow, Periosteum, and Adipose Tissue in Treatment of Partial Growth Arrest in Rabbit

Hui

Teo

et al. 2005

Tissue Engineering

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This study evaluates the ability of MSCs isolated from different origins--bone marrow, periosteum, or fat--to treat partial growth arrest in immature (6-week-old) New Zealand White rabbits. Up to 50% of the medial half of the proximal physis of the tibia was excised in these New Zealand White rabbits. Three weeks later, the bony bridge was excised, and fibrin glue with and without MSCs were transferred into the physeal defect of different rabbits. Contralateral tibias, without undergoing operation, served as self-control. Four groups of rabbits were involved in the study. Each group was injected separately with bone marrow-derived MSCs (group I), periosteum-derived MSCs (group II), fat-derived MSCs (group III), and fibrin glue alone (control, group IV). The rabbits were killed 8 and 16 weeks postoperatively. Clinical, radiological, and histological analyses were subsequently performed. Similar proliferative rates for three MSCs were demonstrated on days 4, 7, and 11 of primary culture. However, MSCs derived from bone-marrow and periosteum appeared to be more homogeneous than that from fat. All MSCs demonstrated chondrogenic and osteogenic differentiation potentials in vitro. The tibias in groups I and II showed significant correction of varus angulation at 16 weeks. However, the varus angulation in group III remained significantly obvious when compared with group I (p < 0.05). The length discrepancies between operated and normal tibiae in groups I, II, and III were significantly corrected compared with control (p < 0.01). In conclusion, bone-marrow and periosteum yielded more homogenous MSCs than fat, providing better correction of physeal arrest in rabbits. The source of MSCs itself could influence the success in the treatment of growth arrest.

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Growth-Plate Chondrocyte Cultures for Reimplantation Into Growth-Plate Defects in Sheep

Cited by 34 publications

References 0 publications

The implantation of cartilaginous and periosteal tissue into growth plate defects

The implantation of cartilaginous and periosteal tissue into growth plate defects

11th Yahya Cohen Memorial Lecture – An In vivo Comparative Study of the Ability of Derived Mesenchymal Stem Cells in the Treatment of Partial Growth Arrest

Comparative Study of the Ability of Mesenchymal Stem Cells Derived from Bone Marrow, Periosteum, and Adipose Tissue in Treatment of Partial Growth Arrest in Rabbit

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