Hair cell regeneration in the avian vestibular epithelium

Weisleder, Pedro; Rubel, Edwin W.

doi:10.1016/0014-4886(92)90211-8

Cited by 70 publications

(36 citation statements)

References 8 publications

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“…Jorgensen and Mathiesen [1988] and Roberson et al [1992] have provided evidence suggesting that new vestibular hair cells are produced at a low rate in the normal adult bird . Weisleder and Rubel [1992] have shown in the bird that repair of the sensory epithelium does occur following pharmacological destruction of vestibular hair cells. The production of new hair cells increases substantially during the repair process.…”

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confidence: 99%

Does Vestibular End-Organ Function Recover after Gentamicin-Induced Trauma in Guinea Pigs?

Bremer

Versnel²,

Hendriksen³

et al. 2014

Audiol Neurotol

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Until 1993 it was commonly accepted that regeneration of vestibular hair cells was not possible in mammals. Two histological studies then showed structural evidence for spontaneous regeneration of vestibular hair cells after gentamicin treatment. There is less evidence for functional recovery going along with this regenerative process; in other words, do regenerated hair cells function adequately? This study aims to address this question, and in general evaluates whether spontaneous functional recovery may occur, in the short or long term, in mammals after ototoxic insult. Guinea pigs were treated with gentamicin for 10 consecutive days at a daily dose of 125 mg/kg body weight. Survival times varied from 1 day to 16 weeks. Vestibular short-latency evoked potentials (VsEPs) to linear acceleration pulses were recorded longitudinally to assess otolith function. After the final functional measurements we performed immunofluorescence histology for hair cell counts. Auditory brainstem responses (ABRs) to click stimuli were recorded to assess cochlear function. As intended, gentamicin treatment resulted in significant loss of utricular hair cells and accompanying declines in VsEPs. Hair cell counts 8 or 16 weeks after treatment did not significantly differ from counts after shorter survival periods. Maximal functional loss was achieved 1-4 weeks after treatment. After this period, only 2 animals showed recovery of VsEP amplitude - all other animals did not reveal signs of regeneration or recovery. In contrast, after initial ABR threshold shifts there was a small but significant recovery. We conclude that spontaneous recovery of otolith function, in contrast to cochlear function, is very limited in guinea pigs. These results support the concept of intratympanic gentamicin treatment where gentamicin is used for chemoablation of the vestibular sensory epithelia.

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confidence: 99%

Does Vestibular End-Organ Function Recover after Gentamicin-Induced Trauma in Guinea Pigs?

Bremer

Versnel²,

Hendriksen³

et al. 2014

Audiol Neurotol

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“…2). It is fairly well established that hair cells of the avian cochlea and vestibular system [Weisleder and Rubel, 1992] die after either aminoglycoside or sound damage in a similar manner to that observed in mammals. However, within 2 weeks after damage the avian hair cells will be replaced by normally functioning hair cells [Saunders et al, 1995;Smolders, 1999] that recover proper neural connections [Cotanche, 1999;Stone and Rubel, 2000].…”

Section: The Stem Cell Niche Within the Inner Earmentioning

confidence: 92%

The Potential Use of Stem Cells for Cochlear Repair

Parker

Cotanche

2004

Audiol Neurotol

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In light of the currently defined characteristics of stem cells, a re-evaluation of hair cell regeneration in birds suggests that there may be a stem cell population located in the inner ear. It is yet to be determined if the mammalian cochlea contains stem cells, but the presence of a mammalian vestibular stem cell population would not appear to be out of the realm of possibility. This paper reviews the latest advances in stem cell biology and suggests that stem cells may be an appropriate biological tool to be used for cochlear repair. The potential use of several types of stem cells, including embryonic, neural and hematopoietic stem cells, as agents for cochlear repair is examined.

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“…BrdU uptake was detected using immunocytochemical techniques, while the presence of tritiated thymidine was determined with autoradiography. Both markers reliably mark S phase cells (Presson and Popper, 1990;Weisleder and Rubel, 1992;Presson et al, 1995).…”

Section: S Phase Markersmentioning

confidence: 99%

“…In fish, the number of hair cells increases substantially over the life of an animal (Corwin, 1981;Popper and Hoxter, 1984;Lombarte et al, 1993). In contrast, continuous production of hair cells in the avian vestibular system is far less than in fish and does not result in a detectable increase in hair cell numbers as the animal matures (Jørgensen and Mathiesen, 1988;Roberson et al, 1992;Weisleder and Rubel, 1992).…”

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confidence: 99%