Halloysite nanotubes as carriers of vancomycin in alginate-based wound dressing

Abstract

The influence of an inorganic support - halloysite nanotubes - on the release rate and biological activity of the antibiotic encapsulated in alginate-based dressings was studied. The halloysite samples were loaded with approx. 10 wt.% of the antibiotic and then encapsulated in Alginate and Gelatin/Alginate gels. The material functionalized with aliphatic amine significantly extended the release of vancomycin from alginate-based gels as compared to that achieved when silica was used. After 24 h, the released amounts of the antibiotic immobilized at silica reached 70%, while for the drug immobilized at halloysite the released amount of vancomycin reached 44% for Alginate discs. The addition of gelatin resulted in even more prolonged sustained release of the drug. The antibiotic was released from the system with a double barrier with Higuchi kinetic model and Fickian diffusion mechanism. Only the immobilized drug encapsulated in Alginate gel demonstrated very good antimicrobial activity against various bacteria. The inhibition zones were greater than those of the standard discs for the staphylococci and enterococci bacteria tested. The addition of gelatin adversely affected the biological activity of the system. The inhibition zones were smaller than those of the reference samples. A reduction in the drug dose by half had no significant effect on changing the release rate and microbiological activity. The toxicity studies of the material with immobilized drug were carried out with and . The material studied had no effect on the living organisms used in the bioassays. The proposed system with a double barrier demonstrated high storage stability.

Effectiveness of Modern Wound Dressing on the Growth of Staphylococcus Aureus Bacteria

“…Dari sepuluh artikel yang ditinjau, semua penelitian yang dilakukan review paper ini menggunakan study design eksperimental (Shahzad et al, 2015;Chen et al, 2017;Ng & Leow, 2015;Jayakumar et al, 2015;Sebria & Amin, 2016;Phaechamud et al, 2016;Kurczewska et al, 2017;Güneş & Tıhmınlıoğlu, 2017;Blandón et al, 2016;Hajimirzababa et al, 2017). Terdapat tiga penelitian menggunakan hidrogel (Shahzad et al, 2015;Chen et al, 2017;Sebria & Amin, 2016), dua penelitian menggunakan natural rubber films dan chitosan films (Phaechamud et al, 2016;Güneş & Tıhmınlıoğlu, 2017), dan satu penelitian menggunakan biofilm (Ng & Leow, 2015), serta empat penelitian menggunakan alginate (Jayakumar et al, 2015;Kurczewska et al, 2017;Blandón et al, 2016;Hajimirzababa et al, 2017).…”

“…Terdapat tiga penelitian menggunakan hidrogel (Shahzad et al, 2015;Chen et al, 2017;Sebria & Amin, 2016), dua penelitian menggunakan natural rubber films dan chitosan films (Phaechamud et al, 2016;Güneş & Tıhmınlıoğlu, 2017), dan satu penelitian menggunakan biofilm (Ng & Leow, 2015), serta empat penelitian menggunakan alginate (Jayakumar et al, 2015;Kurczewska et al, 2017;Blandón et al, 2016;Hajimirzababa et al, 2017).…”

“…Sedangkan Hidrogel GG dapat menghambat bakteri Gram positif, yaitu Staphylococcus Aureus (Sebria & Amin, 2016). Dua artikel yang membahas tentang dressing film topografi menunjukkan aktivitas antimikroba yang baik terhadap Staphylococcus Aureus, Pseudomonas aeruginosa dan Escherichia coli (Phaechamud et al, 2016;Güneş & Tıhmınlıoğlu, 2017 (Jayakumar et al, 2015;Kurczewska et al, 2017;Blandón et al, 2016;Hajimirzababa et al, 2017).…”

“…Karena, Antimikroba yang ada pada balutan luka dapat membunuh bakteri yang ada pada luka (Darwis, Basyir, Hardiningsih, & Chosdu, 2013). Jenis antimikroba yang digunakan dalam modern wound dressing seperti: Cefixime (Shahzad et al, 2015), Tetracycline (Chen et al, 2017), Gentamicin (Ng & Leow, 2015), Penicillin G-P10 (Sebria & Amin, 2016), Tetramethylrodamine (Jayakumar et al, 2015), Gentamicine Sulfate (Phaechamud et al, 2016), Vancomycin Hydrochloride (Kurczewska et al, 2017), Penicillin dan Streptomycin (Güneş & Tıhmınlıoğlu, 2017), Chiprofloxacine (Blandón et al, 2016) …”

Design and characterization of nanocarriers loaded with Levofloxacin for enhanced antimicrobial activity; physicochemical properties, in vitro release and oral acute toxicity

“…Antimicrobial carriers of HNTs have been reported in literature. In a recent study, HNTs were loaded with Vancomycin and further encapsulated in Alginate and Gelatin/Alginate gels as wound dressings to sustain the drug release (Kurczewska et al, 2017). Another research team evaluated the enhanced antibacterial activity against Escherichia coli incorporating halloysite nanotubes (HNTs) and silver into zinc oxide nanoparticles, since neat HNTs exhibit normal bacteria growth (Shu et al, 2017).…”