Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein‐2 in the connective tissue of the palate

Abstract

The purpose of this study was to evaluate whether hard tissue might be formed on dentin surfaces applied with recombinant human bone morphogenetic protein-2 (rhBMP-2) in palatal connective tissue. Fifty-eight dentin blocks were prepared from rat roots, demineralized with 24% EDTA (pH 7.0), applied with 0, 50 and 100 microgram/ml rhBMP-2, and labeled as groups 0, 50 and 100. The dentin blocks were then transplanted into palatal connective tissue of rats, and specimens were prepared at two and four weeks after surgery for histologic and histomorphometric examinations. The results showed that the percentage of newly formed hard tissue in relation to the total dentin block surface length in groups 0, 50 and 100 was 0.0%, 2.8% and 4.4% at two weeks, and 0.0%, 1.6% and 12.8% at four weeks, respectively. New hard tissue formation in groups 50 and 100 was significantly promoted as compared to group 0 (p < 0.01). These findings thus indicate that rhBMP-2 application to dentin enhanced new hard tissue formation on dentin surfaces in the connective tissue of the palate.

In Vitro Manipulation of Cleft Palate Connective Tissue: Setting the Bases of a Proposed New Treatment

“…This finding demonstrates that the cleft palate connective tissue cells respond to external BMP-2 becoming bone forming cells, thus allowing us to suggest that in an in vivo cleft palate model extra bone would be produced from these cells if conveniently induced. Interestingly, the BMP-2 induction of bone occurred in the tgf-␤ 3 null mice palate, similar to the observed in wild type rodents [31,32], indicate that the alteration of the tgf-␤ 3 gene does not modify this tissue response to BMP-2. No differences were observed between BMP-2 treated and untreated cultures at either 24 h or 3 weeks.…”

Influence of Residual Bone on Recombinant Human Bone Morphogenetic Protein‐2–Induced Periodontal Regeneration in Experimental Periodontitis in Dogs

“…Thus, cementum would regenerate on the root‐planed surfaces. Second, mesenchymal progenitor cells originating from gingival connective tissues differentiate into cementoblasts on rhBMP‐2 implantation and would regenerate cementum on the root‐planed surface 38–40 …”

Sonic hedgehog gene-enhanced tissue engineering for bone regeneration

“…While studies involving the use of exogenously administered recombinant BMP-2 and BMP-7 to induce bone regeneration have generally been promising in lower animals 3,4,5 , human studies have demonstrated a large variation in response to recombinant BMP 6 . Additionally, the short in vivo half-life of rBMPs 7 , coupled with the ability of pharmacologic doses of BMPs to stimulate osteoclast production 8 , may limit the usefulness of recombinant BMPs in the clinical setting 9 .…”