Hericium Erinaceus Prevents DEHP-Induced Mitochondrial Dysfunction and Apoptosis in PC12 Cells

Amara, Ines; Scuto, Maria; Zappalà, Agata; Ontario, Maria Laura; Petralia, Antonio; Abid‐Essefi, Salwa; Maiolino, Luigi; Signorile, Anna; Salinaro, Angela Trovato; Calabrese, Vittorio

doi:10.3390/ijms21062138

Cited by 37 publications

(19 citation statements)

References 64 publications

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“…Several studies have shown that H. erinaceus mycelium can stimulate the synthesis of NGF for neuroprotection and prevents neurodegenerative diseases [ 32 ]. Depression is a common neuropsychiatric conditions and abnormal behaviors can be improved by the H. erinaceus mycelium in animals [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Hericium erinaceus mycelium ameliorate anxiety induced by continuous sleep disturbance in vivo

Lee

et al. 2021

BMC Complement Med Ther

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Background Sleep disruption is a major public health issue and may increase the risk of mortality by ten-folds if an individual is sleeping less than 6 h per night. Sleep has changed dramatically during to the COVID-19 pandemic because COVID symptoms can lead to psychological distress including anxiety. Hericium erinaceus mycelium has been widely investigated in both the in vivo studies and clinical trials for its neuroprotective functions because the mycelium contains hericenones and erinacines, which synthesize the nerve growth factor and brain-derived neurotrophic factor (BDNF). Recent in vivo reports have shown showed that erinacine A-enriched Hericium erinaceus mycelium can modulate BDNF/TrkB/PI3K/Akt/GSK-3β pathways to induce an antidepressant-like effect. A large body of evidence indicates that erinacine can pass the blood-brain barrier and suggests its neuroprotective function in both peripheral and central nervous systems. Thus, Hericium erinaceus mycelium may be a dual-function supplement for sleep disruption improvement while sustaining anxiolytic effects. Method To simulate the condition of sleep disruption, the mice were subjected to the tail suspension test (TST) for 15 min every day during the same period for nine consecutive days. Two different doses (75 and 150 mg/kg) of Hericium erinaceus mycelium were administered orally 20 min prior to the TSTs before entering the light period of 12:12 h L:D cycle. All sleep-wake recording was recorded for 24 h using electroencephalogram and electromyogram. The elevated-plus-maze and open-field tests were conducted to record the behavior activities. Results Consecutive TSTs prior to the light period could cause significant sleep disturbance and anxiety behavior in the elevated-plus-maze experiments. Results showed that administration with Hericium erinaceus mycelium at 150 mg/kg ameliorated the rodent anxiety (p < 0.05) and reversed the TST-induced NREM sleep disturbance in the dark period. Conclusion This is the first in vivo study suggesting that Hericium erinaceus mycelium has a dual potential role for anxiety relief through improving sleep disruptions.

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Section: Discussionmentioning

confidence: 99%

Hericium erinaceus mycelium ameliorate anxiety induced by continuous sleep disturbance in vivo

Lee

et al. 2021

BMC Complement Med Ther

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“…In this study, HESAE was shown to attenuate apoptosis following exposure to high-dose corticosterone. Likewise, a biomass preparation of H. erinaceus also showed protective activity against Di-2-ethylhexyl-phthalate (DEHP)-induced apoptosis mediated by caspase activation [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of Hericium erinaceus (Bull.: Fr.) Pers. against high-dose corticosterone-induced oxidative stress in PC-12 cells

Lew

Lim

et al. 2020

BMC Complement Med Ther

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Background Hericium erinaceus is a culinary and medicinal mushroom in Traditional Chinese Medicines. It has numerous pharmacological effects including immunomodulatory, anti-tumour, anti-microbial, anti-aging and stimulation of nerve growth factor (NGF) synthesis, but little is known about its potential role in negating the detrimental effects of oxidative stress in depression. The present study investigated the neuroprotective effects of H. erinaceus standardised aqueous extract (HESAE) against high-dose corticosterone-induced oxidative stress in rat pheochromocytoma (PC-12) cells, a cellular model mimicking depression. Methods PC-12 cells was pre-treated with HESAE for 48 h followed by 400 μM corticosterone for 24 h to induce oxidative stress. Cells in complete medium without any treatment or pre-treated with 3.125 μg/mL desipramine served as the negative and positive controls, respectively. The cell viability, lactate dehydrogenase (LDH) release, endogenous antioxidant enzyme activities, aconitase activity, mitochondrial membrane potentials (MMPs), intracellular reactive oxygen species (ROS) levels and number of apoptotic nuclei were quantified. In addition, HESAE ethanol extract was separated into fractions by chromatographic methods prior to spectroscopic analysis. Results We observed that PC-12 cells treated with high-dose corticosterone at 400 μM had decreased cell viability, reduced endogenous antioxidant enzyme activities, disrupted mitochondrial function, and increased oxidative stress and apoptosis. However, pre-treatment with HESAE ranging from 0.25 to 1 mg/mL had increased cell viability, decreased LDH release, enhanced endogenous antioxidant enzyme activities, restored MMP, attenuated intracellular ROS and protected from ROS-mediated apoptosis. The neuroprotective effects could be attributed to significant amounts of adenosine and herierin III isolated from HESAE. Conclusions HESAE demonstrated neuroprotective effects against high-dose corticosterone-induced oxidative stress in an in vitro model mimicking depression. HESAE could be a potential dietary supplement to treat depression.

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“…curcumin, resveratrol, flavonols present in Ginkgo biloba extracts, polyphenols present abundantly in the leaves and in the ripening fruits of the olive tree, Olea europaea ), as well as mushrooms ( Hericium Erinaceus , Coriolus versicolor ) can significantly modulate Nrf2 and Nrf2-dependent vitagenes expression, showing neuroprotective action. This can potentially resolve pathologies such as AD, PD and also Meniere’s Disease, another degenerative pathology ( Amara et al, 2020 , Trovato et al, 2016a , Trovato et al, 2016b , Trovato Salinaro et al, 2018 , Scuto et al, 2019 ).…”

Section: Molecular Mechanisms Involving Ozone (O 3 mentioning

confidence: 99%

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

Scassellati

Galoforo

Bonvicini

et al. 2020

Ageing Research Reviews

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Highlights Aging and neurodegenerative diseases are characterized by complex and multifactorial aetiology and challenges regarding treatments efficacy and costs persist. Oxygen-ozone (O 2 -O 3 ) therapy is a non-invasive, non-pharmacological, low cost and no-side effect procedure based on antioxidant capacity of O 3 . A plethora of scientific evidence supports other crucial properties of O 3 in physiopathological processes. Nrf2 is one of the principal molecular signalling on which O 3 exercises its antioxidant/anti-apoptotic/pro-autophagy effects, involved in aging and in neurodegenerative disorders. This review provides a consistent rationale to implement future clinical studies to apply the O 2 -O 3 treatment along with other antioxidants (polyphenols, mushrooms) in aging and in neurodegeneration.

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Hericium Erinaceus Prevents DEHP-Induced Mitochondrial Dysfunction and Apoptosis in PC12 Cells

Cited by 37 publications

References 64 publications

Hericium erinaceus mycelium ameliorate anxiety induced by continuous sleep disturbance in vivo

Hericium erinaceus mycelium ameliorate anxiety induced by continuous sleep disturbance in vivo

Neuroprotective effects of Hericium erinaceus (Bull.: Fr.) Pers. against high-dose corticosterone-induced oxidative stress in PC-12 cells

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

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