High genetic diversity of Chinese porcine reproductive and respiratory syndrome viruses from 2016 to 2019

Chen, Nanhua; Xiao, Yanzhao; Ye, Mengxue; Li, Xinshuai; Li, Shubin; Xie, Ningjun; Yue, Wei; Wang, Jialin; Zhu, Jianzhong

doi:10.1016/j.rvsc.2020.04.004

Cited by 33 publications

(24 citation statements)

References 37 publications

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“…PRRSV functional pathogenesis may vary depending on the PRRSV genotypes. In China, the epidemiological studies including ours showed that highly pathogenic (HP)-PRRSV2 (lineage 8) has been the predominant genotype, whereas the NADC30-like PRRSV2 (lineage 1) isolates increasingly appear in recent years, and QYYZ-like PRRSV2 (lineage 3), CH-1a-like PRRSV2 (lineage 5) and PRRSV1 isolates also coexist in Chinese swine herds [47]. HP-PRRSV is most virulent and is therefore the most important genotype associated with the PRRS and the economic loss caused by PRRS.…”

Section: Discussionmentioning

confidence: 79%

The Innate Immune DNA Sensing cGAS-STING Signaling Pathway Mediates Anti-PRRSV Function

Zhang

Luo

et al. 2021

Viruses

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Porcine reproductive and respiratory syndrome virus (PRRSV) modulates host innate immunity which plays a key role against PRRSV infection. As a RNA virus, PRRSV is mainly sensed by innate immune RNA receptors, whereas the role of innate immune DNA sensors in the PRRSV infection has not been elucidated. Here, we investigated the roles of DNA sensing cGAS-STING pathway in both PRRSV infected Marc-145 cells and porcine macrophages. The results show that in Marc-145 cells, the stable expression of STING with or without stimulations exhibited anti-PRRSV activity, and STING knockout heightened PRRSV infection. In CD163-3D4/21 porcine macrophages, either expression of STING or stimulation of cGAS-STING signaling obviously suppressed PRRSV infection, whereas in STING knockdown macrophages, the PRRSV infection was upregulated. Our results clearly demonstrate that the host cGAS-STING signal exerts an important antiviral role in PRRSV infection.

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Section: Discussionmentioning

confidence: 79%

The Innate Immune DNA Sensing cGAS-STING Signaling Pathway Mediates Anti-PRRSV Function

Zhang

Luo

et al. 2021

Viruses

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“…In China, even though PRRSV1 isolates were sporadically detected in recent years, PRRSV2 isolates were obviously predominant [ 5 ]. In 1995, PRRSV2 was first identified in Chinese swine herds [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Since 2017, NADC34-like PRRSV2 isolates have also been detected [ 9 ]. To make matters worse, the coexistence of distinct PRRSV isolates within one pig herd or even within one pig has been identified in the field [ 5 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

Chen

Tian

et al. 2021

Vet Res

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Due to the substantial genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV), commercial PRRS vaccines fail to provide sufficient cross protection. Previous studies have confirmed the existence of PRRSV broadly neutralizing antibodies (bnAbs). However, bnAbs are rarely induced by either natural infection or vaccination. In this study, we designed and synthesized a consensus sequence of PRRSV2 ORF2-6 genes (ORF2-6-CON) encoding all envelope proteins based on 30 representative Chinese PRRSV isolates. The ORF2-6-CON sequence shared > 90% nucleotide identities to all four lineages of PRRSV2 isolates in China. A chimeric virus (rJS-ORF2-6-CON) containing the ORF2-6-CON was generated using the avirulent HP-PRRSV2 JSTZ1712-12 infectious clone as a backbone. The rJS-ORF2-6-CON has similar replication efficiency as the backbone virus in vitro. Furthermore, pig inoculation and challenge studies showed that rJS-ORF2-6-CON is not pathogenic to piglets and confers better cross protection against the virulent NADC30-like isolate than a commercial HP-PRRS modified live virus (MLV) vaccine. Noticeably, the rJS-ORF2-6-CON strain could induce bnAbs while the MLV strain only induced homologous nAbs. In addition, the lineages of VDJ repertoires potentially associated with distinct nAbs were also characterized. Overall, our results demonstrate that rJS-ORF2-6-CON is a promising candidate for the development of a PRRS genetic engineered vaccine conferring cross protection.

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“…To evaluate the evolutionary relationships between our PPV genomes and other PPV genomes, the obtained 20 complete genomes and 35 representative PPV genomes (5 genomes for each genotype) were aligned by the ClustalX 2.0. Genome-based phylogenetic trees were constructed using MEGA 6.06 as we previously described ( 40 ). In details, phylogenetic analysis was performed based on the aligned sequence using the neighbor-joining method and the maximum composite likelihood model.…”

Section: Methodsmentioning

confidence: 99%

A Systematic Investigation Unveils High Coinfection Status of Porcine Parvovirus Types 1 through 7 in China from 2016 to 2020

Xiao

Qiu

et al. 2021

Microbiol Spectr

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High genetic diversity of Chinese porcine reproductive and respiratory syndrome viruses from 2016 to 2019

Cited by 33 publications

References 37 publications

The Innate Immune DNA Sensing cGAS-STING Signaling Pathway Mediates Anti-PRRSV Function

The Innate Immune DNA Sensing cGAS-STING Signaling Pathway Mediates Anti-PRRSV Function

Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate

A Systematic Investigation Unveils High Coinfection Status of Porcine Parvovirus Types 1 through 7 in China from 2016 to 2020

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