High performance liquid chromatographic determination of N-butyryl glucosamine in rat plasma

Aghazadeh‐Habashi, Ali; Carran, John; Anastassiades, Tassos; Jamali, Fakhreddin

doi:10.1016/j.jchromb.2005.01.034

Cited by 21 publications

(24 citation statements)

References 6 publications

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“…2. This reaction was reported by Aghazadeh-Habashi et al in 2002 [34]. The derivatization protocol was also carried out on acetyl GlcN and glucose, L-glutamic acid, and BSA that were presumed as contaminants in the solution after hydrochlorination process.…”

Section: Resultsmentioning

confidence: 82%

“…Starting from hydrolysis at 100ºC for 6 h by using 6 N HCl, it is followed by evaporation in a vacuum oil system, reduction by using borohydride at room temperature, and finally continued by the derivatization process. Derivatization of GlcN by using 1-NITC have been reported by Aghazadeh-Habashi et al (2002) [34]. Reaction of GlcN with 1-NITC using a standard aqueous solution, produced a derivative that exhibits favorable UV absorbing properties by bonding the amino functional group of GlcN.…”

Section: Introductionmentioning

confidence: 89%

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Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Sitanggang

Wang

2009

Biotechnol Bioproc E

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A rapid method for the determination of fungal glucosamine (GlcN) from ^ëéÉêÖáääìë=ëé BCRC 31742 was developed. The hydrochlorination process using microwave effectively reduced reaction time needed for GlcN analysis. The analytical method consisted of two steps: (1) hydrochlorination of fungal cells and (2) derivatization process. Fungal GlcN hydrochloride was reacted with 1-napthyl isothiocyanate (1-NITC) as the derivatizing agent to enhance the sensitivity of GlcN and so to achieve high resolution. This method was specific for quantification of GlcN hydrochloride at the wavelength of 230 nm. The standard deviation and relative error of the analytical results were less than 5%. By using microwave heating, the reaction time of hydrochlorination process was shortened from 24 h to 3 min. Thus, the overall time needed for analyzing GlcN from fungal sources was reduced from 5 h (thermal method) to 2 h (microwave method). © KSBB hÉóïçêÇëW=ãáÅêçï~îÉI=ÑìåÖ~ä=ÖäìÅçë~ãáåÉI=ãáÅêçÄá~äI=âáåÉíáÅëI=ÅÜêçã~íçÖê~éÜóI=çéíáãáò~íáçå= = = = =

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Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 89%

Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Sitanggang

Wang

2009

Biotechnol Bioproc E

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“…During initial experiments, four derivative reagents including 1-phenyl-3-methyl-5-pyrazolone (PMP), FMOC-CL, naphthyl isothiocyanate and PITC were respectively utilized to analyze GlcN and its analogue. PMP had already been used to derivatize aldoses and Nacetylhexosamines (Honda et al, 1989) and N-butyryl glucosamine (Aghazadeh-Habashi et al, 2005). However, PMP reacted with not only the target compounds but also all other compounds containing aldehyde groups, because the reaction-target of PMP was aldehyde groups.…”

Section: Discussionmentioning

confidence: 99%

Optimizing high‐performance liquid chromatography method for quantification of glucosamine using 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate derivatization in rat plasma: application to a pharmacokinetic study

Chen

et al. 2008

Biomedical Chromatography

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A sensitive and reliable HPLC method with fluorescence detection based on the precolumn derivatization of glucosamine with 6-aminoquinolyl-N-hydroxylsuccinimidyl carbamate (AQC) was established for the quantitative determination of glucosamine in rat plasma. The plasma protein was precipitated by acetonitrile, followed by vortex mixing and centrifugation. The supernatant was divided into the organic layer and aqueous layer by adding sodium chloride, and then the aqueous layer was derivatized with AQC in 0.2 M borate buffer of pH 8.8 before the HPLC analysis. An amino acid analysis column (3.9 x 150 mm, 4 microm) was applied, with 140 mM sodium acetate buffer (pH = 5.25) and acetonitrile as mobile phase at a flow rate of 1 mL/min. A linear correlation coefficient of 0.9987 was calculated within the range of 0.1-30 microg/mL of the standard curve for glucosamine. The limit of detection was 30 ng/mL. The intra- and inter-day precisions (as RSD) were less than 7.38 and 12.72%, respectively. The intra- and inter-day accuracy ranged from 91.8 to 110.0%. Extraction recoveries of glucosamine in plasma were more than 90%. The validated method was successfully applied for the quantitative determination of glucosamine in rat plasma and evaluation for pharmacokinetic study of glucosamine. It was also possible to be applied for the quantitative determination of other compounds containing amino group in biological samples.

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“…This may be attributed to a low gastrointestinal absorption rather than a large first-pass effect in the liver, which metabolizes a substantial quantity of the absorbed glucosamine into smaller compounds, such as H 2 O, CO 2 , and urea [7,10].…”

Section: Is Glucosamine Pharmacologically Active?mentioning

confidence: 99%

The use of glucosamine therapy in osteoarthritis

Zerkak

Dougados

2004

Curr Rheumatol Rep

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Glucosamine products have been used extensively for the management of pain in osteoarthritis. This paper reviews the most recent clinical and experimental studies regarding its efficacy and safety. Although clinical trials include methodologic flaws and publication bias, glucosamine is likely an effective therapy for the symptomatic management of osteoarthritis. In turn, definite proof for chondromodulating effect requires well-conducted clinical trials. In North America, glucosamine is an over-the-counter dietary supplement and preparations made by different manufacturers may vary. There is also a need to standardize this therapy and allow practitioners to give patients suitable advice. An ongoing long-term clinical trial in the US will possibly permit to investigate the clinical relevance of these results and give appropriate recommendations.

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High performance liquid chromatographic determination of N-butyryl glucosamine in rat plasma

Cited by 21 publications

References 6 publications

Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Optimizing high‐performance liquid chromatography method for quantification of glucosamine using 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate derivatization in rat plasma: application to a pharmacokinetic study

The use of glucosamine therapy in osteoarthritis

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