High-pressure liquid chromatography of steroidal alkaloids

Hunter, Irving R.; Walden, Mayo K.; Wagner, Joseph R.; Heftmanń, Erich

doi:10.1016/s0021-9673(00)86785-0

Cited by 36 publications

(6 citation statements)

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“…Table I), plants containing high levels of these compounds in the leaves will also give high immunoassay values. However, since both solasodine and tomatidine can be distinguished easily by T L C [9], the few RIA-selected samples with high immunoassay values can be checked qualitatively for the occurrence of solasodine o r tomatidine, and thus unambiguous interpretation of the results is achieved. In our opinon the immuno-procedures will be of considerable value for the mass screening of fresh as well as herbarium plant material for commercially valuable natural ~r o d u c t s as well as for screening programs aimed at the detection of individual specimens which contain less than average of an undesirable compound.…”

Section: Khasianum Contain Most Of the Solaso-mentioning

confidence: 99%

“…Colorimetric determinations in the 1C-100 ~g range are also in use [8] but are equally unspecific and the TLC methods ted compounds described in the literature are, at best, semi-quantitative [9]. Since solasodine cannot be detected with sufficient sensitivity with H P L C detectors, this method [lo] t o date is of interest only for preparative purposes.…”

Section: Introductionmentioning

confidence: 99%

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Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹

Weiler¹,

Krüger²,

Zenk³

1980

Planta Med

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A rapid, specific and sensitive radioimmunoassay for the determination of the steroidal alkaloid solasodine is described. The assay allows the detection of as little as 0.7 ng of solasodine glycosides (sola-' sonine, solarnargine) in crude plant extracts. The detailed distribution of solasodine in vegetative and generative parts of plants of Solanum laciniatum was investigated, and several thousand S. laciniatum plants were screened for individuals with high solasodine content. Plants which contained as much as 3.1 Oh of solasodine in the leaves were selected for further breeding. A taxonomic survey of the occurrence of solasodine in ca. 250 different species of the genus Solan-' Pan 13 in the series: "Use of lmmunoassay in Plant Sciences". For part 12 see: E. W. WEILER, Planta (in press).um using herbarium material is given. 0.5 mg of a herbarium sample is more than sufficient for quantitative analysis.

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Section: Khasianum Contain Most Of the Solaso-mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹

Weiler¹,

Krüger²,

Zenk³

1980

Planta Med

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“…To systematically examine the preclinical pharmacokinetic studies of jervine, a sensitive, fast, and validated analytical method for the determination of jervine in biological fluids is necessary. Previous studies were focus on the extraction, separation, and quantification of jervine and other constituents in Veratrum species using high-performance liquid chromatography (HPLC) [ 12 , 13 ]. Up to the present moment, only a few bioanalytical methods have been published for the detection of jervine in biological fluids.…”

Section: Introductionmentioning

confidence: 99%

A New UPLC-MS/MS Method Validated for Quantification of Jervine in Rat Plasma and the Study of Its Pharmacokinetics in Rats

Chen

Weng

2019

Journal of Analytical Methods in Chemistry

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The aim of this study was to develop an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to assess the concentration of jervine in rat plasma and its pharmacokinetics. Diazepam was used as internal standard (IS). The chromatographic separation of jervine and IS was carried out on an UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with a flow rate of 0.4 mL/min. A mixture of acetonitrile and water (0.1% formic acid) was used as a mobile phase. The UPLC-MS/MS was equipped with an electrospray ionization (ESI), adopting multiple reactive monitoring mode to determine jervine in rat plasma. The retention times of jervine and the internal standard were 1.71 and 2.13 min, respectively. The calibration curve of jervine ranged between 1 and 1000 ng/mL. The lower limit of quantitation (LLOQ) was 1 ng/mL, and the lower limit of determination (LLOD) was 0.2 ng/mL. The accuracy was ±6%; the interday precision and intraday precision were no more than 9%. The recovery was higher than 90.3%, and the matrix effect was lower than 10%. The UPLC-MS/MS method was successfully developed and used for the application of the pharmacokinetic study. The primary pharmacokinetic parameters of jervine in this study were as follows: the AUC(0–∞) was 969.3 ± 277.7 ng/mL·h, the Cmax was 506.6 ± 192.8 ng/mL, the CL/F was 1.7 ± 0.5 L/h/kg, and the t1/2 was 3.4 ± 1.2 h.

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“…Hunter et al used HPLC to separate several steroidal alkaloids. 16 Philip et al applied this technique to analyze and separate solasodine, solasonine and solamargine. They investigated and optimized many chromatographic parameters in order to have better separation of these alkaloids.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an RP-HPLC Method for Determination of Solasodine, A Steroidal Alkaloid

Khandani¹,

Mehrabani²,

Sharififar³

et al. 2018

JYP

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Objective:The aim of the present study was to optimize an RP-HPLC method for determination of solasodine, a steroidal alkaloid with various pharmacologic properties, in herbal medicinal preparations. Methods: Various chromatographic parameters including the composition of mobile phase and its organic and pH modifiers, mode of elution and column temperature were investigated using analytical C 18 columns as stationary phase. Acetonitrile and methanol were examined in combination with deionized water and different aqueous buffers as mobile phases. Results: A mixture of methanol: KH 2 PO 4 buffer (pH 2.5) at the ratio of 75:25 %v/v in an isocratic elution mode produced more symmetric, sharper peaks with better retention times and capacity factor than that of acetonitrile: KH 2 PO 4 buffer. The optimum column temperature was found at 25°C. The standard calibration curve showed a very good linearity (R 2 =0.9966) in the range of 1-25 μg/ml with acceptable inter-day and intra-day precisions and accuracy.In addition, the LOD and the LOQ of the method were determined at 0.2 and 0.7 μg/mL, respectively. Conclusion: This developed and validated method is simple and quite rapid and can be used for routine determination of solasodine in samples such as traditional medicinal preparations.This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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High-pressure liquid chromatography of steroidal alkaloids

Cited by 36 publications

References 4 publications

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹

A New UPLC-MS/MS Method Validated for Quantification of Jervine in Rat Plasma and the Study of Its Pharmacokinetics in Rats

Development and Validation of an RP-HPLC Method for Determination of Solasodine, A Steroidal Alkaloid

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High-pressure liquid chromatography of steroidal alkaloids

Cited by 36 publications

References 4 publications

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens1

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens1

A New UPLC-MS/MS Method Validated for Quantification of Jervine in Rat Plasma and the Study of Its Pharmacokinetics in Rats

Development and Validation of an RP-HPLC Method for Determination of Solasodine, A Steroidal Alkaloid

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Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹