High-Throughput Production of Single-Cell Microparticles Using an Inkjet Printing Technology

Xu, Tao; Kincaid, Helen; Atala, Anthony; Yoo, James J.

doi:10.1115/1.2903064

Cited by 115 publications

(76 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As these properties are important for the droplet formation [76][77][78], properties of the substrate is also important as the factors including but not limited to wettability of the surface, surface roughness as well as viscous forces [79]. Furthermore, the M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 12 spreading of the droplet occurs faster than its polymerization in a typical bioprinting setup [61] and the parameters associated with the crosslinking mechanism (i.e., ionic crosslinker solution concentration) influences the spreading behavior of the droplet [55]. Droplet-substrate interactions broadly consist of two regimes from a fluid mechanics perspective [77,80].…”

Section: Droplet-substrate Interactionsmentioning

confidence: 98%

See 1 more Smart Citation

A comprehensive review on droplet-based bioprinting: Past, present and future

2016

View full text Add to dashboard Cite

Droplet-based bioprinting (DBB) offers greater advantages due to its simplicity and agility with precise control on deposition of biologics including cells, growth factors, genes, drugs and biomaterials, and has been a prominent technology in the bioprinting community. Due to its immense versatility, DBB technology has been adopted by various application areas, including but not limited to, tissue engineering and regenerative medicine, transplantation and clinics, pharmaceutics and high-throughput screening, and cancer research. Despite the great benefits, the technology currently faces several challenges such as a narrow range of available bioink materials, bioprinting-induced cell damage at substantial levels, limited mechanical and structural integrity of bioprinted constructs, and restrictions on the size of constructs due to lack of vascularization and porosity. This paper presents a first-time review of DBB and comprehensively covers the existing DBB modalities including inkjet, electrohydrodynamic, acoustic, and micro-valve bioprinting. The recent notable studies are highlighted, the relevant bioink biomaterials and bioprinters are expounded, the application areas are presented, and the future prospects are provided to the reader.

show abstract

Section: Droplet-substrate Interactionsmentioning

confidence: 98%

“…The bioprinted cells retained their functional phenotype and genotype, and proliferation capacity [17,[54][55][56]. In summary, bioprinting studies pertaining TIJ bioprinting technology to date have been to assess its impact on the functionality of bioprinted cells.…”

Section: A N U S C R I P Tmentioning

confidence: 98%

A comprehensive review on droplet-based bioprinting: Past, present and future

2016

View full text Add to dashboard Cite

show abstract

“…As a pioneer of 3D printing, Charles W. Hull introduced the stereolithography method by uniting thin layers of certain materials with ultraviolet light [51]. Later, the technology was improved leading to new methods, such as laser printing [52] and ink printing [53]. In addition, soft lithographic methods were developed, which utilize 'stamping' of each cell-containing layer by creating layer-specific molds and stacking of these layers to provide a 3D shape [54,55].…”

Section: D Bioprintingmentioning

confidence: 99%

In vitro skin three-dimensional models and their applications

Klicks

Molitor

Ertongur-Fauth

et al. 2017

JCB

View full text Add to dashboard Cite

Abstract. Skin fulfils a plethora of eminent physiological functions ranging from physical barrier over immunity shield to the interface mediating social interaction. Prone to several acquired and inherited diseases, skin is therefore a major target of pharmaceutical and cosmetic research. The lack of similarity between human and animal skin and rising ethical concerns in the use of animal models have driven the search for novel realistic three-dimensional skin models. This review provides a survey of contemporary skin models and compares them in terms of applicability, reliability, cost and complexity.Keywords: Skin, phenotypic screening, 3D models, pharmaceutical research Skin -composition and functionHuman skin covers an area of almost 2 m 2 in the adult and consists of the three major layers, subcutis, dermis, and epidermis. The subcutis is composed of adipose and epithelial cells. It harbours blood vessels, neurites of peripheral neurons, Vater-Pacini mechanosensors, and, partially, also sweat glands and hair follicles. It connects the skin to periosteum and fascia, absorbs forces, and mediates thermal insulation. The dermis supplies the epidermis with mechanical support and nutrients. It is stratified into an inner, reticular, and an outer, papillary, zone. The dermis houses most sebaceous glands, sweat glands, hair follicles, smooth muscle cells, and capillary beds and, thus, regulates skin moisture, body temperature, and performs the secretory function of skin. The papillary layer is characterized by relatively loose connective tissue, where Meissner corpuscles sense touch. Immune cells, particularly mast cells and dendritic cells, are patrolling in the papillary layer and mediate local inflammatory reactions and immune surveillance. Finally, dermal fibroblasts secrete extracellular matrix (ECM) and basement membrane components. These are primarily collagens I and III, and a proteoglycan-rich ground substance [1]. The resulting ECM mediates tensile strength of the dermis. The dermo-epidermal junction is centered around a special basement membrane. This is composed of a laminin/collagen IV scaffold and further typical basement membrane components such as perlecans and nidogens [1].The epidermis is a squamous epithelium of 50-100 m thickness. It is devoid of blood vessels but contains keratinocytes, Merkel cell mechanosensors, Langerhans immune cells, and melanocytes. The 1 These authors contributed equally.

show abstract

“…The controlled volumes of the ink materials are delivered to predefined locations by such ink bioprinters. The first inkjet printers used for the bioprinting applications were modified versions of the ink-based 2D printers [7]. There are three major types of drop-on-demand bioprinters: thermal, piezoelectric, and mechanical.…”

Section: Ink-jet Bioprintersmentioning

confidence: 99%

3D Printing for Tissue Engineering Applications

Hacıoglu¹,

Yılmazer²,

Üstündağ³

2018

Journal of Polytechnic

View full text Add to dashboard Cite

The goal of tissue engineering is to create functional tissues and organs for regenerative therapies, and total organ transplantation. Bioprinting tissues are one of the most attractive approaches for tissue engineering and regenerative medicine fields. Fabrication of a complex structure via bioprinting requires layer-by-layer fabrication strategy. Bioprinting is mainly based on three processes; imaging and computer aided the design of the tissue that we wanted to print, the production of bio-ink with the selection of proper substances, the choice of a proper bioprinter depending on the product that we want, for fabrication of scaffold and/or tissues. In recent years the 3D bioprinting technology has been developed and several approaches appear by the researchers. The approaches are biomimicry, autonomous self-assembly and mini-tissue building blocks. In this study, current and future potential applications of 3D printing for the tissue engineering and regenerative medicine will be discussed.

show abstract

High-Throughput Production of Single-Cell Microparticles Using an Inkjet Printing Technology

Cited by 115 publications

References 22 publications

A comprehensive review on droplet-based bioprinting: Past, present and future

A comprehensive review on droplet-based bioprinting: Past, present and future

In vitro skin three-dimensional models and their applications

3D Printing for Tissue Engineering Applications

Contact Info

Product

Resources

About