HIV-1 Subtypes and Primary Antiretroviral Resistance Mutations in Antiretroviral Therapy Naive HIV-1 Infected Individuals in Turkey

Sayan, Murat; Willke, Ayşe; Özgüneş, Nail; Sargın, Fatma

doi:10.7883/yoken.66.306

Cited by 16 publications

(11 citation statements)

References 20 publications

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“…The most frequently detected non-B subtype was CRF02_AG, which is a circulating recombinant form in West Africa. The fact that nearly 50 % of the subtypes in this cohort of heterosexual patients were non-B subtypes and that the origin of infection was predominant in Turkey reflects a high prevalence of HIV-1 non-B infections in Turkey [21,22].…”

Section: Resultsmentioning

confidence: 82%

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HIV-1 subtypes and drug resistance profiles in a cohort of heterosexual patients in Istanbul, Turkey

Köksal

Beka

Lübke

et al. 2015

Med Microbiol Immunol

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Turkey is seeing a steady rise in rates of HIV infection in the country. The number of individuals with HIV/AIDS was greater than 7000 in 2014 according to data released by the Ministry of Health, and heterosexual contacts were reported to be the main transmission routes. Istanbul has the highest number of reported cases of HIV infection. The aim of the study was to determine the prevalence of HIV-1 drug resistance in 50 heterosexual patients from Istanbul. The most prevalent subtype was found to be subtype B (56.2 %). Resistance-associated mutations were found in 14 patients with 6/14 patients being therapy-experienced and 8/14 therapy naive at the time point of analysis. With increasing number of patients who require treatment and the rapid up-scaling of the antiretroviral therapy in Turkey, HIV-1 drug resistance testing is recommended before starting treatment in order to achieve better clinical outcomes.

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Section: Resultsmentioning

confidence: 82%

“…Anti-HIV drugs have become broadly available for the treatment of HIV-positive patients in Turkey, and the genetic diversity of HIV-1 has been analysed in recent studies showing that besides subtype B, the CRFs 02_AG, 01_AE and 03_AB are circulating [2,22,37].…”

mentioning

confidence: 99%

HIV-1 subtypes and drug resistance profiles in a cohort of heterosexual patients in Istanbul, Turkey

Köksal

Beka

Lübke

et al. 2015

Med Microbiol Immunol

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“…The lower prevalence of NRTI backbone treatment mutations and the higher prevalence of TAMs may be associated with an increase in HIV-1 infection in men who have sex with men (MSM) in the past 2 years (in 2012, 23% of patients were MSM, compared to 43% in this study). 12 Significant proportions of the patients with TDRMs have revertant TAMs, which transmit but do not reduce drug susceptibility. The largest proportion of TAMs included first by position of 215C/D/F/S/Y and second D67N, K219Q, or M41L mutations.…”

Section: Discussionmentioning

confidence: 99%

“…HIV-1 cDNA synthesis was done with the First Strand cDNA Synthesis Kit (Thermo Scientific Inc., Fermentas, Lithuania) including the M-MuLV reverse transcriptase enzyme. The PCR conditions were 95°C for 10 min, and then 45 cycles consisting of 95°C for 45 s, 55°C for 45 s, and 72°C for 45 s. 12 All PCR products were purified using the Highly Pure PCR Product Purification Kit (Roche Diagnostics GmbH, Mannheim, Germany) and directly sequenced with ABI PRISM 310 Genetic Analyzer equipment using the DYEnamic ET Terminator Cycle Sequencing Kit (Amersham Pharmacia Biotech Inc., Piscataway, NJ). The following thermal protocol …”

Section: Pcr Amplification and Sequence Analysismentioning

confidence: 99%

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HIV-1 Transmitted Drug Resistance Mutations in Newly Diagnosed Antiretroviral-Naive Patients in Turkey

Sayan

Sargın²,

İnan

et al. 2016

AIDS Research and Human Retroviruses

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HIV-1 replication is rapid and highly error-prone. Transmission of a drug-resistant HIV-1 strain is possible and occurs within the HIV-1-infected population. In this study, we aimed to determine the prevalence of transmitted drug resistance mutations (TDRMs) in 1,306 newly diagnosed untreated HIV-1-infected patients from 21 cities across six regions of Turkey between 2010 and 2015. TDRMs were identified according to the criteria provided by the World Health Organization's 2009 list of surveillance drug resistance mutations. The HIV-1 TDRM prevalence was 10.1% (133/1,306) in Turkey. Primary drug resistance mutations (K65R, M184V) and thymidine analogue-associated mutations (TAMs) were evaluated together as nucleos(t)ide reverse transcriptase inhibitor (NRTI) mutations. NRTI TDRMs were found in 8.1% (107/1,306) of patients. However, TAMs were divided into three categories and M41L, L210W, and T215Y mutations were found for TAM1 in 97 (7.4%) patients, D67N, K70R, K219E/Q/N/R, T215F, and T215C/D/S mutations were detected for TAM2 in 52 (3.9%) patients, and M41L + K219N and M41L + T215C/D/S mutations were detected for the TAM1 + TAM2 profile in 22 (1.7%) patients, respectively. Nonnucleoside reverse transcriptase inhibitor-associated TDRMs were detected in 3.3% (44/1,306) of patients (L100I, K101E/P, K103N/S, V179F, Y188H/L/M, Y181I/C, and G190A/E/S) and TDRMs to protease inhibitors were detected in 2.3% (30/1,306) of patients (M46L, I50V, I54V, Q58E, L76V, V82A/C/L/T, N83D, I84V, and L90M). In conclusion, long-term and large-scale monitoring of regional levels of HIV-1 TDRMs informs treatment guidelines and provides feedback on the success of HIV-1 prevention and treatment efforts.

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Dynamics of HIV/AIDS in Turkey from 1985 to 2016

Sayan

Hınçal²,

Şanlıdağ

et al. 2017

Qual Quant

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In this paper, we formulated a mathematical model that studies the dynamics of HIV/ AIDS in Turkey from 1985 to 2016. We find two equilibrium points, disease free equilibrium and endemic equilibrium. Global stability analysis of the equilibria was conducted using Lyapunov function which depends on the basic reproduction ratio R 0 . If R 0 \ 1, the disease free equilibrium point is globally asymptotically stable, and if R 0 C 1 the endemic equilibrium point is globally asymptotically stable. We computed and predicted the basic reproduction ratios across all the years. It was found out that there were flaws in the exact values of R 0 which is related to the poor registration system of HIV/AIDS in Turkey. Hence, there is need for the government to improve the system in order to cover the actual cases of the disease. The increase of the basic reproduction ratio over the years also shows the need for the relevant authorities to adopt appropriate control measures in combating the disease.

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HIV-1 Subtypes and Primary Antiretroviral Resistance Mutations in Antiretroviral Therapy Naive HIV-1 Infected Individuals in Turkey

Cited by 16 publications

References 20 publications

HIV-1 subtypes and drug resistance profiles in a cohort of heterosexual patients in Istanbul, Turkey

HIV-1 subtypes and drug resistance profiles in a cohort of heterosexual patients in Istanbul, Turkey

HIV-1 Transmitted Drug Resistance Mutations in Newly Diagnosed Antiretroviral-Naive Patients in Turkey

Dynamics of HIV/AIDS in Turkey from 1985 to 2016

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