HMG-CoA reductase limits artemisinin biosynthesis and accumulation in Artemisia annua L. plants

Ram, Mauji; Khan, Mather Ali; Jha, Prabhakar; Khan, Salim; Kiran, Usha; Ahmad, Malik M.; Javed, Saleem; Abdin, M. Z.

doi:10.1007/s11738-010-0470-5

Cited by 56 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, inhibition in artemisinin accumulation was observed in the presence of fosmidomycin, the competitive inhibitor of 1-deoxy-Dxylulose 5-phosphate reductase (DXR). Artemisin is a sesquiterpene-lactone isolated from the aerial parts of Artemisia annua plants that is biosynthesised by MVA pathway (Ram et al 2010).…”

Section: Resultsmentioning

confidence: 99%

Influence of mevinolin on chloroplast terpenoids in Cannabis sativa

Mansouri

Salari

2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Plants synthesize a myriad of isoprenoid products that are required both for essential constitutive processes and for adaptive responses to the environment. Two independent pathways for the biosynthesis of isoprenoid precursors coexist within the plant cell: the cytosolic mevalonic acid (MVA) pathway and the plastidial methylerythritol phosphate (MEP) pathway. In this study, we investigated the inhibitory effect of the MVA pathway on isoprenoid biosynthesized by the MEP pathway in Cannabis sativa by treatment with mevinolin. The amount of chlorophyll a, b, and total showed to be significantly enhanced in treated plants in comparison with control plants. Also, mevinolin induced the accumulation of carotenoids and α-tocopherol in treated plants. Mevinolin caused a significant decrease in tetrahydrocannabinol (THC) content. This result show that the inhibition of the MVA pathway stimulates MEP pathway but none for all metabolites.

show abstract

Section: Resultsmentioning

confidence: 99%

Influence of mevinolin on chloroplast terpenoids in Cannabis sativa

Mansouri

Salari

2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Esculin content in leaf extract was quantified by RP-HPLC method [36]. In conclusion, result presented here clearly illustrate the positive impact of calcium phosphate nanoparticles mediated trasformation on biosynthesis of esculin via natural mean without the application of biological or other previously used systems having low transformation frequency [41]. 8).…”

Section: Evaluation Of Enhanced Biosynthesis Of Esculinmentioning

confidence: 66%

“…pDNA/chitosan nano-complexes as gene delivery system were reported by investigator for gene therapy [41]. Necrosis of leaf segments in explants observed within 24h of incubation in untransformed plants.…”

Section: Transformation Of C Intybus Via Calcium Phosphate Nanopartimentioning

confidence: 98%

Plant Bio-transformable HMG-CoA Reductase Gene Loaded Calcium Phosphate Nanoparticle: In vitro Characterization and Stability Study

R¹,

Alvari²,

Samim³

et al. 2013

Current Drug Discovery Technologies

View full text Add to dashboard Cite

Encapsulation of plasmid DNA in nanoparticle is expected to enhance the stability of DNA, reproducibility and frequency of the genetic transformation in plants. Here we report the formulation of HMG Co-A reductase gene loaded calcium phosphate nanoparticles (Cap nanoparticles) and their in-vitro, in-vivo characterization. The developed Cap nanoparticles were characterized by DSC, FT-IR, and XRD. Developed Cap nanoparticles were spherical in shape having the particle size and zeta potential in the range of 10.86±0.09nm to 33.42±0.18nm and -25.5±0.07mV to -31.7±0.07mV (for Cap-I to Cap-IV). DNA releasing in acidic media showed, initially slow release followed by fast release with a maximum release of Cap-I (95.77±1.39%)> Cap-II (87.32±2.07%)> Cap-III (76.54±2.01%)> Cap-IV (72.93±1.75%) over 60min. Cap nanoparticles were quite stable at storage condition of 40±0.5°C/75±5%RH, 25±0.5°C/60±RH, 4±0.5°C/ambient humidity and the integrity of pDNA encapsulated was confirmed by gel electrophoresis. Compared to wild type C. intybus, transformation efficiency and enhanced biosynthesis of esculin with the DNA nanoparticles in C. intybus were about 10% and 71%, respectively. Antioxidant activity capacity of the biotransformed plants was significantly higher than the normal plant due to high accumulation of esculin.

show abstract

“…Transcriptional levels of HMGR and FDS are of particular relevance because previous studies have shown that by increasing expression of these genes, the amount of artemisinin increases several-fold (Chen et al, 2000;Aquil et al, 2009;Jing et al, 2009;Liu et al, 2010;Arsenault et al, 2010;Wang et al, 2011;Lin et al, 2011;Alam et al, 2014). On the other hand, Ram et al (2010) studied the in vivo activity of HMGR and its influence on artemisinin and reported that HMGR activity limits the accumulation of artemisinin biosynthesis in A. annua. Therefore, HMGR was reported as a key regulatory enzyme of the mevalonate pathway.…”

Section: Expression Analysis Of Artemisinin Biosynthetic Genesmentioning

confidence: 99%