Horseradish peroxidase-catalyzed hydrogelation for biomedical applications

Khanmohammadi, Mehdi; Dastjerdi, Mahsa Borzouyan; Ai, Arman; Ahmadi, Aslan; Godarzi, Arash; Rahimi, Abbas; Ai, Jafar

doi:10.1039/c8bm00056e

Cited by 129 publications

(143 citation statements)

References 182 publications

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“…tuneable gelation rates under physiologically relevant conditions. [58] Due to the respective nature of the oxidants used by HRP and laccase, HRP has been shown to have faster gelation rates than laccase in the cross-linking of tyrosine-modified PVA hydrogels. [59] 5 | LYSYL OXIDASE-THE ECM RELOADED…”

Section: Transglutaminasementioning

confidence: 99%

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

et al. 2020

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Intermolecular cross-linking is one of the most important techniques that can be used to fundamentally alter the material properties of a polymer. The introduction of covalent bonds between individual polymer chains creates 3D macromolecular assemblies with enhanced mechanical properties and greater chemical or thermal tolerances. In contrast to many chemical cross-linking reactions, which are the basis of thermoset plastics, enzyme catalysed processes offer a complimentary paradigm for the assembly of cross-linked polymer networks through their predictability and high levels of control. Additionally, enzyme catalysed reactions offer an inherently 'greener' and more biocompatible approach to covalent bond formation, which could include the use of aqueous solvents, ambient temperatures, and heavy metal-free reagents. Here, we review recent progress in the development of biocatalytic methods for polymer cross-linking, with a specific focus on the most promising candidate enzyme classes and their underlying catalytic mechanisms. We also provide exemplars of the use of enzyme catalysed cross-linking reactions in industrially relevant applications, noting the limitations of these approaches and outlining strategies to mitigate reported deficiencies.

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Section: Transglutaminasementioning

confidence: 99%

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

et al. 2020

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“…Skin substitutes are introduced to overcome the lack of skin donor tissue in sever damaged skin like chronic ulcers, major burns, and extensive full-thickness wounds, where auto-grafts are not available to replace (Grainger, 2015;Khanmohammadi et al, 2018;Mansbridge, 2009;Newberry et al, 2018). This restriction typically results in tissue infection which postpones wound healing, raise treatment costs for patients as well as increase risk of death (Grainger, 2015;Mansbridge, 2009;Newberry et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Impact of exosome‐loaded chitosan hydrogel in wound repair and layered dermal reconstitution in mice animal model

Nooshabadi

Khanmohamadi

Valipour

et al. 2020

J Biomedical Materials Res

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107

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Combat or burn injuries are associated with a series of risks, such as microbial infection, an elevated level of inflammatory response, and pathologic scar tissue formation, which significantly postpone wound healing and also lead to impaired repair. Skin engineering for wound healing requires a biomimetic dressing substrate with ideal hydrophilicity, holding antioxidant and antimicrobial properties. In addition, available bioactive specification is required to reduce scar formation, stimulate angiogenesis, and improve wound repair. In this study, we successfully fabricated chitosan (Ch)–based hydrogel enriched with isolated exosome (EXO) from easy‐accessible stem cells, which could promote fibroblast cell migration and proliferation in vitro. Full‐thickness excisional wound model was used to investigate the in vivo dermal substitution ability of the fabricated hydrogel composed Ch and EXO substrates. Our finding confirmed that the wounds covered with Ch scaffold containing isolated EXO have nearly 83.6% wound closure ability with a high degree of re‐epithelialization, whereas sterile gauze showed 51.5% of reduction in wound size. In summary, obtained results imply that Ch‐glycerol‐EXO hydrogel construct can be utilized at the full‐thickness skin wound substitution and skin tissue engineering.

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“…In general, chitosan is obtained from the thermochemical deacetylation of chitin in the presence of alkali or from certain fungi naturally (4,6). It has been widely used in pharmaceutical and biomedical applications, due to its biological properties such as biocompatibility, low toxicity, and good characteristics for cell culture systems (6)(7)(8)(9)(10). Moreover, chitosan could be crosslinked with negatively charged molecules through electrostatic interactions such as sulfur-containing amino acids, glycosaminoglycans, proteoglycans, and growth factors (10,11).…”

Section: Introductionmentioning

confidence: 99%

Alginate-Based Hydrogel Containing Taurine-Loaded Chitosan Nanoparticles in Biomedical Application

et al. 2019

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The hydrogel efficacy of taurine-loaded chitosan nanoparticle/alginate hydrogel was investigated for controlled release of the taurine substrate, which is known as an antioxidative drug. The composition of the fabricated hydrogels was explored by Fouriertransform infrared spectroscopy. The swelling ability and degradation rate of hydrogels were also analyzed in phosphate-buffered saline at the physiological condition and alginate lyase for a period of 21 days. Moreover, morphologies and structure of hydrogels and cells were determined using a scanning electron microscope. The possible cytotoxicity of the fabricated hydrogel was carried out by seeding endometrial stem cells on hydrogels. The results demonstrated that hydrogel of chitosan nanoparticle/alginate hydrogel successfully controlled the release of taurine. We observed that the chitosan nanoparticle/alginate hydrogel possessed adjust swelling ability and degradation rate as compared to neat alginate hydrogel. The results proved that the chitosan nanoparticle/alginate hydrogel is non-cytotoxic and could be utilized as a promising composition in tissue engineering and drug delivery systems.

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Horseradish peroxidase-catalyzed hydrogelation for biomedical applications

Cited by 129 publications

References 182 publications

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

Impact of exosome‐loaded chitosan hydrogel in wound repair and layered dermal reconstitution in mice animal model

Alginate-Based Hydrogel Containing Taurine-Loaded Chitosan Nanoparticles in Biomedical Application

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