HPTLC Fingerprint Profile (Phenolics) of Selected<i> Cyathea</i> Species from Western Ghats, South India

Janakiraman, N.; Antonysamy, Johnson Marimuthu alias

doi:10.1155/2016/6420371

Cited by 10 publications

(6 citation statements)

References 9 publications

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“…Janakiraman and Johnson [12] employed mobile phase and spraying reagents provided the good separation and high resolution ( Figure 4). Among the observed tannins, the tannins with Rf value 0.01, 0.04 and 0.11 were considered as known tannins (10.40%) in the TLC system.…”

Section: Resultsmentioning

confidence: 99%

“…HPTLC separations was performed by the standard method [10,11]. 3 µL of methanolic extracts of A. aethiopicum were loaded for the separations.The separation of phenolics, flavonoids and tannins was achieved by adopting the mobile phase and spraying reagents of Janakiraman and Johnson methods [12].The toluene-acetone-formic acid (4.5:4.5:1) and Folin-Ciocalteu reagent was employed as mobile phase and spraying reagents for phenolics. The mobile phase toluene-acetone-formic acid (4.5:4.5:1) and spraying reagent 1% ethanolic aluminium chloride was used for the separations of flavonoids.…”

Section: Hptlc Analysismentioning

confidence: 99%

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Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC

et al. 2020

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The present study was aimed to validate the phenolic, flavonoids, alkaloids and tannins profile of Aspleniuma ethiopicum (Burm. f.) Becherer methanolic extracts using HPTLC (High-performance thin-layer chromatography). The chromatographic separation was performed using the standard method. The separation methodology was optimized and phytoconstituents of A. aethiopicum methanolic extracts were separated. The methanolic extract of A. aethiopicum showed several bands identified as known phenolic compounds. The obtained flavonoids profiles were identified. HPTLC alkaloids profile of A. aethiopicum revealed 11 types of alkaloids. The tannin profile of methanolic extracts of A. aethiopicum demonstrated 11 different types of tannins. The observed profiles will be used as phytomarker to identify the chemical constituents of A. aethiopicum methanolic extracts. These profiles will act as a fingerprint of A. aethiopicum and help us to distinguish from its adulterants. The observed profile will help us to identify the crude drugs and improve the therapeutic potentials of A. aethiopicum.

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Section: Resultsmentioning

confidence: 99%

Section: Hptlc Analysismentioning

confidence: 99%

Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC

et al. 2020

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“…C. gigantea , a tree fern belonging to the family Cyatheaceae, possesses immense and diverse ethnomedicinal importance [3–5]. Preliminary phytochemical analysis of different extract of the fern was carried out which have brought foreword the presence of various bioactive secondary metabolites viz., steroids, alkaloids, phenolic groups, cardiac glycosides, flavonoids, saponins, tannins and terpenoids [26]. This presence of bioactive secondary metabolites has investigated to study the plant for its various bioactivity.…”

Section: Discussionmentioning

confidence: 99%

“…The antimicrobial property also may be due to the presence of coumarin, which is an antimicrobial agent naturally present in plant [27]. In addition some other isolated compounds viz., 2-methylbutane-1,4-diol, 3-(1-ethoxyethoxy) may also impart to the antibacterial property of the plant [26].…”

Section: Discussionmentioning

confidence: 99%

Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms

Nath

Talukdar

Bhattacharya

et al. 2019

BMC Complement Altern Med

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BackgroundRapid emergence of multidrug resistant (MDR) organisms in hospital and community settings often result into treatment failure, thus leading the clinicians with fewer treatment options. Cyathea gigantea, an ethnomedicinally important fern used in cuts and wound infections. So, if this medicinal plant is used in treating the MDR infections then it might bring certain relief in future treatment options.MethodsAntibacterial activity of C. gigantea against MDR bacteria was assed using well diffusion and broth microdilution methods to determine the diameters of growth inhibition zones, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Synergistic activity was also determined with the conventional antibiotics by disc diffusion method followed by FIC index of each of the tested antibiotic was calculated. The active extract was then subjected to fractionation by column chromatography and antibacterial activity was done with each of the collected fractions.ResultsCrude extract of C. gigantea was found to be active against all the tested organisms. The MIC was 200 μg/ml against Gram-positive i.e., Staphylococcus aureus ATCC 25923 and 400 μg/ml against Gram-negative i.e., Escherichia coli ATCC 25922 and Pseudomonas aeruginosa PAO1, while the MBC was 400 μg/ml in case of Gram-positive and 800 μg/ml for Gram-negative. The synergistic activity revealed that the plant extract increased the antibacterial property of the studied antibiotics and the FIC index showed that significant synergistic activity was shown by ciprofloxacin followed by tetracycline, ampicillin and oxacillin. Antibacterial activity with the fractionated extract showed that the FR II, FR III and FR IV were active against both Gram-positive and Gram-negative bacteria, whereas FR I, FR V and FR VI did not show antibacterial property against any of the tested bacteria.ConclusionsExtracts of C. gigantea was found active against both selected Gram-positive and Gram-negative organisms and thus offers the scientific basis for the traditional use of the fern. The present study also provides the basis for future study to validate the possible use against multidrug resistant organisms.

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“…According to WHO guidelines, chromatographic fingerprints such as TLC, HPTLC, HPLC, GC, and hyphenated techniques can be used in the identification and determination of adulteration in herbal drugs 9,10 . HPTLC fingerprint can be utilized as a phytochemical marker and as an estimator of genetic variability in plant population 11 . The present study aims to point out the presence of major phytoconstituents and to develop a quality control standard fingerprint for different extractive fractions of C. latifolia stem.…”

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confidence: 99%

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2019

IJPSR

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Cissus latifolia Lam. (Vitaceae) is a woody climber with leafopposed tendrils. It is a medicinal plant used in the traditional system of medicine for the treatment of various ailments. The present study focused on the identification and qualitative determination of phytoconstituent types and establishment of the HPTLC fingerprint profile of the hot and cold extracts of C. latifolia. Preliminary phytochemical screening was done to identify the class of compounds present. HPTLC analyses of eight different extracts were carried out with the most suitable mobile phase system using the Camag HPTLC instrument consisting of Linomat-V automated spotter having a 100 μl syringe connected to a nitrogen cylinder, twintrough developing chamber, scanner-III and viewing cabinet with dual wavelength UV lamps (Camag, Muttenz, Switzerland). Qualitative phytochemical screening revealed the presence of flavonoids, coumarins, tannins, alkaloids, steroids, terpenoids, saponins, quinines, anthraquinones and phenol in the stem of C. latifolia. The HPTLC profiling of eight different extracts showed the presence of alkaloids, flavonoids, phenols, saponins, and tannins with different R f values. The results of preliminary phytochemical screening and HPTLC fingerprint obtained from this study can be used as a reference for the standardization and quality control of Cissus latifolia stem. INTRODUCTION: According to Angiosperm Phylogeny Group IV classification, the family Vitaceae consists of two subfamilies Vitoideae (Eaton) and Leeoideae (Burmeister) 1 and Cissus is the largest genus in the family with about 350 species 2. Cissus L. is seen widely distributed in the tropical regions such as Africa (ca.135 species), Southern Asia (ca. 85 species), Australia (ca.12 species) and the Americas (77 species) 3. C. latifolia is a woody climber with leaf-opposed tendrils, which may be modified to form an inflorescence.

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HPTLC Fingerprint Profile (Phenolics) of Selected Cyathea Species from Western Ghats, South India

Cited by 10 publications

References 9 publications

Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC

Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC

Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms

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