hsa_circ_0071106 can be used as a diagnostic marker for type 2 diabetes

Abstract

We explored has_circ_0071106 as a diagnostic marker for type 2 diabetes (T2DM) in south China, and predicted the functional mechanism of the target circRNA. A total of 107 T2DM patients and 107 healthy reference persons were included as the research objects. In the first stage, the circRNA microarray was used to detect the peripheral blood of 4 T2DM and 4 control groups to screen the differential expression profile of circRNA. In the second stage, four circRNAs were screened from the differential expression profiles of circRNA, and real-time polymerase chain reaction (Q-PCR) technology was used to verify the blood samples of 103 T2DM and 103 controls. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in bioinformatics were used to predict the functional mechanism of target circRNA. Lastly,we found that has_circ_0071106 increase the risk of T2DM (OR=2.819 (95% CI: 1.415~5.616)). Thearea under the ROCcurve has_circ_0071106 was 0.690, the sensitivity was 62.1%, and the specificity was 69.9%. The function prediction results showed that has_circ_0071106 was involved in biological processes such as protein binding, gene transcription, and may be involved in the pathway of hsa-miR-29a-5p regulating diabetes, has_circ_0071106 may be used as a diagnostic marker for T2DM.

hsa-miR-607, lncRNA TUG1 and hsa_circ_0071106 can be combined as biomarkers in type 2 diabetes mellitus

“…The function prediction results showed that hsa_circ_0071106 was involved in MAPK signaling pathway that regulates insulin signal transduction after the enrichment of the KEGG pathway. 7 Jiang et al 29 found miR-607 is judged as a potential biomarker for patients with pancreatic ductal carcinoma, indicating that miR-607 plays a vital role in pancreatic islet tissue. We found that lncRNA TUG1 increase the risk of T2DM.…”

“…Many studies have found that some circRNAs can be used as diagnostics biomarkers of pre-diabetes and T2DM, such as hsa_circ_0054633, hsa_ circ_0071106. 7,8 miRNAs are called microRNAs, which are small single-stranded RNA molecules with only 14-25 nucleotides in length. The genetic regulation involved in miRNA has been found in more and more studies.…”

“…To ensure that they were significantly different between the T2DM group and the control group, we also used the ∆C T (∆C T = C T, Target -C T, internal reference ) method to calculate relative expression. 7 Then circBank (http://www.circbank.cn/), starBase (http:// starbase.sysu.edu.cn/) and circIteractome (https://circinteractome.nia.nih.gov/) databases were used to predict the miRNA interacting with hsa_circ_0071106. And the relationship between miRNAs were described by a network diagram using Cytoscape software and the results are shown in Figure 1.…”

Significance of serum miR‐29a in the occurrence and progression of diabetic nephropathy: A cross‐sectional study

“… 26 , 27 Moreover, as an important miRNA, miR‐29a is expressed and enriched in pancreatic beta cells in mice and humans, and previous studies have shown that miR‐29a is significantly upregulated in diabetes models. 28 , 29 , 30 However, the expression levels of miR‐29a in the serum of DN patients remain unknown. Therefore, to clarify the level of serum miR‐29a in patients with DN and further evaluate its potential relationship with the occurrence and progression of DN, we used qRT‐PCR to detect the expression levels of miR‐29a in the serum of patients with simple T2DM, patients with DN, and healthy subjects.…”

Circular RNAs in diabetes mellitus and its complications

“…Hsa_circ_0071106 is upregulated in the T2DM group and may increase the risk of T2DM by sponging hsa-miR-29a-5p. The area under the ROC curve for this circRNA was 0.690 ( 48 ). Additionally, the areas under the curve (AUCs) for hsa_circ_CCNB1 and circ_0009024 were 0.9255 and 0.9592, respectively ( 47 ).…”