Human cell lines that elaborate colon-stimulating activity for the marrow cells of man and other species

Abstract

We have established two human cell lines which elaborate colony- stimulating activity (CSA) for at least four species: man, mouse, rabbit, and dog. One, GCT, was isolated from a lung metastasis of a fibrous histiocytoma; the other, RC4, from a monocyte-enriched fraction of normal blood. Medium conditioned by either GCT or RC4 cells was more potent in stimulating human marrow growth in vitro than was monocyte-conditioned medium or human leukocyte feeder layers. Fractionation of cell-line-conditioned medium by Sephacryl S-200 chromatography indicated that the maximum activity of the CSA for human marrow cells is eluted within the range of 30,000–40,000 daltons. These cells lines provide a continuous source of large quantities of conditioned medium for purification of CSA. Moreover, the invariable growth-supporting activity for all species tested and the high potency of cell-line CSA facilitates studies of its elaboration and biologic effects.

Establishment of a csf‐producing cell line from a human gastric carcinoma and characteristics of the csf produced by that cell line

“…Colony cell types were identified by cytochemical analysis. After fixing the whole culture specimen, staining for naphthol AS-D chloroacetate esterase and alphanaphthyl butyrate esterase (Sigma Chemical Co., St. Louis, MO) was performed according to the method described by Kubora et al [19]. Scoring for granulocyte colonies consisting of naphthol AS-D chloroacetate esterase positive cells (G-colonies), macrophage colonies consisting of alphanaphthyl butyrate esterase positive cells (M-colonies) and mixed colonies consisting of granulocytes and macrophages (GM) was carried out from these permanent samples.…”

Release of hemopoietic factors by normal human T cell lines with either suppressor or helper activity

“…The in vitro growth and differentiation of hemopoietic progenitor cells depends upon the addition of regulatory molecules derived from the conditioned media (CM) of several normal cell types as well as from the supernatants of some neoplastic cell lines (Di Persio et al, 1978;Burgess and Metcalf, 1980;Golde et al, 1980;Ruscetti et al, 1982;Tarella et al, 1982). The substances inducing the in vitro growth of myeloid precursors are collectively termed colony-stimulating activity (CSA) (Metcalf, 1977), while those specific for the most immature erythroid progenitors are indicated as burst-promoting activity (BPA) (Iscove, 1978).…”

A High Level of Colony‐Stimulating Activity in a Lung Cancer Patient with Extensive Leucocytosis, and the Establishment of a CSA Producing Cell Line (KONT)

“…Several human CSA producing cell lines in vitro have been reported. Di Persio et al (1978) isolated human monocyte-like cell lines (RC-4 & GCT) from a monocyte-enriched fraction of normal blood and from a lung metastasis of fibrous histiocytoma. T3M-1 by Okabe et a1 (1978) originated from a human squamous cell carcinoma of the oral cavity.…”

“…Several sources of colonystimulating activity (CSA) which cause the proliferation of granulopoietic cells have been recognized in humans, including the supernatant of human peripheral leucocytes (Iscove et a1 1971, Chervenick & Boggs 1970, spleen cells (Paran et a1 1970, embryonic kidneys (Brown & Carbone 1971, active lymphocytes (Cerny 1974, Cline & Golde 1974, Ruscetti & Chervenick 1975 and placenta (Burgess et a1 1977). Furthermore, several human CSA producing cell lines in vitro have been reported (Di Persio et al 1978, Okabe et al 1978, Golde et a1 1978, Yunis et a1 1978. In this report we describe a patient with lung cancer associated with marked leucocytosis (where the tumor material produced CSA) and the establishment of a CSA producing cell line (KONT cells).…”