Hydrogels in Regenerative Medicine

Slaughter, Brandon V.; Khurshid, Shahana S.; Fisher, Omar Z.; Khademhosseini, Ali; Peppas, Nicholas A.

doi:10.1002/adma.200802106

Cited by 2,405 publications

(1,962 citation statements)

References 271 publications

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“…In this case, hydrogel mechanics alter the ability of a cell to cluster tethered ligands and exert tension, leading to different fate decisions (for example, adipogenic fate in softer gels and osteogenic fate in stiffer gels). Several further examples also demonstrate that 3D hydrogels support the growth of a wide variety of tissues 15 ; however, the synthesized tissue properties are often inferior to native tissue, for a range of reasons possibly related to the simplicity of these basic designs.…”

Section: Static Hydrogels That Mimic Biophysical Cuesmentioning

confidence: 99%

Moving from static to dynamic complexity in hydrogel design

2012

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Hydrogels are water-swollen polymer networks that have found a range of applications from biological scaffolds to contact lenses. Historically, their design has consisted primarily of static systems and those that exhibit simple degradation. However, advances in polymer synthesis and processing have led to a new generation of dynamic systems that are capable of responding to artificial triggers and biological signals with spatial precision. These systems will open up new possibilities for the use of hydrogels as model biological structures and in tissue regeneration.H ydrogels are water-swollen polymer networks that have been used for many decades, with applications as varied as contact lenses and super-absorbant materials. As the field of biomedical engineering has developed, hydrogels have become a prime candidate for application as molecule delivery vehicles and as carriers for cells in tissue engineering, owing to their ability to mimic many aspects of the native cellular environment (for example, high water content, mechanical properties that match soft tissues). Traditional hydrogels, formed through the covalent and non-covalent crosslinking of polymer chains, were regarded as relatively inert materials, providing a simple biomimetic three-dimensional (3D) environment, either for tissue production by local resident cells or for positioning of cells delivered in vivo. However, the simplicity of these materials may have in fact hindered their application, restricting cellular interactions with the environment and preventing uniform extracellular matrix (ECM) production and proper tissue development. In addition, these materials were limited to modelling static environments and lacked the spatiotemporal dynamic properties relevant for complex tissue processes. Fortunately, during the last decade the concepts of hydrogel design and cellular interaction have evolved, shedding light on how they may control cell behaviour, particularly for tissue engineering applications.Hydrogels with a range of mechanical properties, and capable of incorporating a wide range of biologically relevant molecules, from individual functional groups to multidomain proteins, are currently in development 1 . In addition, hydrogels are being designed with spatial heterogeneity, to either replicate properties in native tissue structures or to produce constructs with distinct regionally specific cell behavior 2 . As a result, studies to date have clearly demonstrated the possibility of creating well-defined microenvironments with control over the 3D presentation of signals to cells 3 . However, recently there has been a focus on the concept of hydrogels that exhibit dynamic complexity. These materials should evolve with time and in response to

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Section: Static Hydrogels That Mimic Biophysical Cuesmentioning

confidence: 99%

Moving from static to dynamic complexity in hydrogel design

2012

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“…Various hybrid materials combined as co-polymers, polymer blends and polymer-ceramic blends have also shown efficacy [67][68][69][70][71][72][73]. Advanced hydrogels, naturally derived collagen and gelatin gels as well as synthetic polyethylene glycol and poly-vinyl alcohol-based hydrogels, serve as matrices for other products and mimic the extracellular matrix topography [74][75][76]. Biomaterials with immunomodulatory strategies, such as artificial extracellular matrices (ECMs) (hydrogels, ECM coatings) and materials with surface property modulation, have the ability to modify the immune function and improve bone repair and regeneration [25,77].…”

Section: Biomaterialsmentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

130

116

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“…Коллагены I и III типов синтезируются фибробластами и преимуществен-но представлены в коже, костной ткани, связках, хрящах, сухожилиях. Они взаимодействуют с различными клетками и регулируют их миграцию и пролиферацию [5,6]. Колла-ген III типа начинает синтезироваться в ране через 10 ч по-сле повреждения, в дальнейшем он заменятся на коллаген I типа [7].…”

Section: обоснованиеunclassified

Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers

Zaitseva¹,

Tokmakova²,

Voronkova³

et al. 2017

Diabetes mellitus

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ФГБУ Национальный медицинский исследовательский центр эндокринологии Минздрава России, Москва ОБОСНОВАНИЕ. Процессы заживления ран нижних конечностей замедлены при сахарном диабете (СД). В различных ис-следованиях показана роль контроля диабета, цитокинов и факторов роста в этих процессах. Однако роль генов, определя-ющих пролиферацию клеток, синтез коллагена, формирование здоровой грануляционной ткани, остается малоизученной. ЦЕЛЬ.Изучить особенности экспрессии генов коллагенов на различных фазах раневого процесса у лиц с нейропатиче-ской формой синдрома диабетической стопы (СДС).МЕТОДЫ. В проспективное исследование включены 4 больных с нейропатической формой СДС после проведенной хи-рургической обработки, у которых были взяты образцы тканей для морфологического и генетического исследования на 0, 10 и 15-е сутки местного лечения с целью оценки экспрессии генов коллагенов COL1A1, COL1A2, COL3A1. Стадия раневого процесса оценивалась по гистологической картине.РЕЗУЛЬТАТЫ. В ходе исследования подтверждено, что размер ран включенных в исследование больных уменьшился на 8,8±7% через 10 дней местного лечения и на 18,3±8% через 15 дней терапии. Согласно данным гистологического исследования биоптатов раневого ложа, через 10 дней у всех больных отмечалась тенденция к уменьшению воспали-тельного инфильтрата, к нарастанию количества фибробластоподобных клеток, фиксировалось наличие созревающей грануляционной ткани и появление формирующихся волокон соединительной ткани. Однако через 15 дней в тканях всех пациентов обнаружена сохраняющаяся воспалительная инфильтрация, несмотря на формирование зрелой грану-ляционной ткани. По результатам генетического исследования на 10-е сутки местного лечения ран выявлена тенден-ция к увеличению экспрессии гена COL1A1 в 3,2±1,3 раза, экспрессии гена COL1A2 в среднем в 2,0±1,0 раз, а экспрессии гена COL3A1 -в 1,25±1,1 раза по сравнению с исходными показателями. На 15-е сутки местного лечения отмечается тенденция к снижению экспрессии генов COL1A1 и COL1A2: по сравнению с исходными данными экспрессия COL1A1 снизилась в 1,7±0,6 раза, а экспрессия генов COL1A2 и COL3A1 уменьшилась в 2,5±2 раза и в 20,0±3 раза соответственно. ЗАКЛЮЧЕНИЕ.Экспрессия генов коллагенов (COL1A1, COL1A2, COL3A1) более выражена в фазу пролиферации и снижается к ее окончанию, что подтверждается клинической картиной и данными морфологического исследования.КЛЮЧЕВЫЕ СЛОВА: сахарный диабет; раны; СДС; репарация; гены; экспрессия GENETIC PARAMETERS OF WOUND HEALING IN PATIENTS WITH NEUROPATIC DIABETIC FOOT ULCERS© BACKGROUND.Tissue repair processes are impaired in diabetic foot ulcers (DFUs). Previous research has shown that glycaemic control, cytokines and growth factors play an important role in wound healing. Emerging evidence also suggests that genes play a role via their regulation of cell proliferation, collagen synthesis and granulation tissue formation.

show abstract

Hydrogels in Regenerative Medicine

Cited by 2,405 publications

References 271 publications

Moving from static to dynamic complexity in hydrogel design

Moving from static to dynamic complexity in hydrogel design

The rational use of animal models in the evaluation of novel bone regenerative therapies

Genetic parameters of wound healing in patients with neuropatic diabetic foot ulcers

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