Hydrogen peroxide-induced oxidative stress, acetylcholinesterase inhibition, and mediated brain injury attenuated by<i>Thymus algeriensis</i>

Guesmi, Fatma; Houda, Bellamine; Landoulsi, Ahmed

doi:10.1139/apnm-2018-0107

Cited by 23 publications

(16 citation statements)

References 55 publications

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“…The reduction of NOX-1 by both extracts may contribute to their protective effects against neuropathic pain. Our findings are consistent with those of a study that reported that TA extract has antioxidant potential and neuroprotective effect in a rat model of H2O2induced brain injury 14 . According to previous studies, the sciatic nerve injury was reported to be responsible for the increase in gene expression of iNOS and NO in spinal cord microglial cells by the 5 th day post-surgery.…”

Section: Discussionsupporting

confidence: 93%

Thymus algeriensis and Thymus fontanesii exert neuroprotective effect against chronic constriction injury-induced neuropathic pain in rats

Rezq

Alsemeh

D’Elia

et al. 2020

Sci Rep

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We have previously demonstrated that the Thymus algeriensis and Thymus fontanesii extracts have powerful anti-inflammatory, antipyretic, and analgesic effects against acute pain models. We profiled their chemical composition and found many phenolic acids, flavonoids, and phenolic diterpenes. In this work, we investigated their antioxidant properties on HaCaT cells exposed to UVA-induced oxidative stress and examined their effects against chronic neuropathic pain and the underlying mechanisms. Through a rat chronic constriction injury (CCI) model, we induced chronic neuropathic pain by placing 4 loose ligatures around the right sciatic nerve for 14 days. Thermal and mechanical hyperalgesia in addition to cold and dynamic allodynia were tested on the day before surgery and on the 7th and 14th post-surgery days. Key markers of the nitrosative and oxidative stresses, in addition to markers of inflammation, were measured at day 14 post surgery. Histopathological examination and immunostaining of both synaptophysin and caspase-3 of sciatic nerve and brain stem were also performed. Results of this study showed that T. algeriensis extract suppresses UVA oxidative stress in HaCaT cells via activation of the Nrf-2 pathway. Both extracts attenuated hyperalgesia and allodynia at 7- and 14-days post-surgery with more prominent effects at day 14 of surgery. Their protective effects against neuropathic pain were mediated by inhibiting NOX-1, iNOS, by increasing the enzyme activity of catalase, and inhibition of inflammatory mediators, NF-κB, TNF-α, lipoxygenase, COX-2 enzymes, and PGE2. Furthermore, they improved deleterious structural changes of the brainstem and sciatic nerve. They also attenuated the increased caspase-3 and synaptophysin. The data indicate that both extracts have neuroprotective effects against chronic constriction injury-induced neuropathic pain. The observed protective effects are partially mediated through attenuation of oxidative and nitrosative stress and suppression of both neuroinflammation and neuronal apoptosis, suggesting substantial activities of both extracts in amelioration of painful peripheral neuropathy.

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Section: Discussionsupporting

confidence: 93%

Thymus algeriensis and Thymus fontanesii exert neuroprotective effect against chronic constriction injury-induced neuropathic pain in rats

Rezq

Alsemeh

D’Elia

et al. 2020

Sci Rep

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“…Cell apoptosis and autophagy are two important biological processes, which can be enhanced by oxidative stress [27]. H 2 O 2 has been widely utilized to construct oxidative stress injury model in many diseases [28,29]. In the present study, we also construct H 2 O 2 -induced cell injury model as the foundation for exploration of the effect of SNGH16 on the pathogenesis of SCI.…”

Section: R E T R a Cmentioning

confidence: 94%

RETRACTED ARTICLE: Long non-coding RNA SNHG16 reduces hydrogen peroxide-induced cell injury in PC-12 cells by up-regulating microRNA-423-5p

Liu

Chen

Zhu

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Functions of long non-coding RNAs (lncRNAs) have been widely probed in spinal cord injury (SCI). But, the influences of lncRNA-small nucleolar RNA host gene 16 (lncRNA-SNHG16) is still not well documented in SCI. The study explored the impacts of SNHG16 on H 2 O 2-injured PC-12 cells. PC-12 cells were disposed with H 2 O 2 , cell viability, apoptosis, autophagy and ROS level were detected. RT-qPCR was executed to explore SNHG16 or miR-423-5p expression in H 2 O 2-stimulated cells. After transfection with pc-SNHG16 or miR-423-5p inhibitor, the functions of SNHG16 and miR-423-5p in H 2 O 2-injured cells were studied. AMPK and ERK1/2 pathways were finally assessed by western blot. We found that H 2 O 2 evoked cell injury in PC-12 cells, and repressed SNHG16 was observed in H 2 O 2-disposed cells. Overexpressed SNHG16 prominently alleviated H 2 O 2-induced cell injury as indicated by repressing cell apoptosis, autophagy and ROS level. Additionally, SNGH16 enhanced miR-423-5p expression, and miR-423-5p inhibition abrogated the protective effect of SNGH16 on H 2 O 2-injured PC-12 cells. SNGH16 mediated AMPK and ERK1/2 pathways via up-regulating miR-423-5p in H 2 O 2-injured PC-12 cells. In conclusion, these findings indicated that SNGH16 reduced H 2 O 2-evoked cell injury by mediating miR-423-5p in PC-12 cells. The findings might uncover the effect of SNHG16 on SCI, which provide a new reference for remedying SCI. HIGHLIGHTS 1. H 2 O 2 evokes cell injury in PC-12 cells; 2. SNHG16 reduces H 2 O 2-induced cell injury in PC-12 cells; 3. SNGH16 enhances miR-423-5p expression in H 2 O 2-stimulated PC-12 cells; 4. MiR-423-5p inhibition abrogates the protective effect of SNGH16 in PC-12 cells; 5. SNGH16 mediates AMPK and ERK1/2 pathways by up-regulating miR-423-5p.

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“…The effect of H 2 O 2 is largely mediated by hydroxyl radicals (OH • ) generated by the Fenton reaction, which is catalyzed by Fe 2+ [ 55 ]. Previous studies have shown that Cu(II) also has an important role in the Fenton reaction to generate ROS [ 44 , 45 , 56 ].…”

Section: Resultsmentioning

confidence: 99%

Attenuation of Oxidative Stress by Cannabinoids and Cannabis Extracts in Differentiated Neuronal Cells

Raja

Ahmadi

Costa³

et al. 2020

Pharmaceuticals

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In this proof-of-concept study, the antioxidant activity of phytocannabinoids, namely cannabidiol (CBD) and Δ9- tetrahydrocannabinol (THC), were investigated using an in vitro system of differentiated human neuronal SY-SH5Y cells. The oxidative stress was induced by hydrogen peroxide, as reactive oxygen species (ROS). Alzheimer’s disease (AD)-like pathological conditions were mimicked in vitro by treating the differentiated neuronal cells with amyloid-β1–42 (Aβ1–42) in the presence of Cu(II). We showed that THC had a high potency to combat oxidative stress in both in vitro models, while CBD did not show a remarkable antioxidant activity. The cannabis extracts also exhibited a significant antioxidant activity, which depended on the ratio of the THC and CBD. However, our results did not suggest any antagonist effect of the CBD on the antioxidant activity of THC. The effect of cannabis extracts on the cell viability of differentiated human neuronal SY-SH5Y cells was also investigated, which emphasized the differences between the bioactivity of cannabis extracts due to their composition. Our preliminary results demonstrated that cannabis extracts and phytocannabinoids have a promising potential as antioxidants, which can be further investigated to develop novel pharmaceuticals targeting oxidative stress therapy.

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Hydrogen peroxide-induced oxidative stress, acetylcholinesterase inhibition, and mediated brain injury attenuated byThymus algeriensis

Cited by 23 publications

References 55 publications

Thymus algeriensis and Thymus fontanesii exert neuroprotective effect against chronic constriction injury-induced neuropathic pain in rats

Thymus algeriensis and Thymus fontanesii exert neuroprotective effect against chronic constriction injury-induced neuropathic pain in rats

RETRACTED ARTICLE: Long non-coding RNA SNHG16 reduces hydrogen peroxide-induced cell injury in PC-12 cells by up-regulating microRNA-423-5p

Attenuation of Oxidative Stress by Cannabinoids and Cannabis Extracts in Differentiated Neuronal Cells

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