Hydrogen sulfide counteracts chlorophyll loss in sweetpotato seedling leaves and alleviates oxidative damage against osmotic stress

Abstract

In this paper, effect of NaHS, a hydrogen sulfide (H 2 S) donor on chlorophyll and antioxidant metabolism in seedling leaves of sweetpotato under osmotic stress was investigated. With the enhancement of osmotic stress, which was mimicked by PEG-6000, chlorophyll in seedling leaves of sweetpotato (Ipomoea batatas) decreased dramatically. At 15% PEG (w/v), chlorophyll concentration reached only 50% compared with that of the controls. The osmotic-induced decrease in chlorophyll concentration could be alleviated by spraying exogenous H 2 S donor, NaHS in a dose-dependent manner, while little visible symptoms were observed in leaves sprayed with NaHS under control conditions. It was also shown that H 2 S or HS -rather than other sulfur-containing components derived from NaHS contributed to the protective role against chlorophyll degradation during osmotic stress. Further studies showed that NaHS spraying dramatically promoted the activities of superoxide dismutase, catalase, ascorbate peroxidase and decreased that of lipoxygenase and the concentrations of hydrogen peroxide (H 2 O 2 ) and malondialdehyde. In addition, concentrations of endogenous H 2 S in NaHS-sprayed seedlings were higher than that in water-spraying control under osmotic stress. These data indicated that H 2 S plays a protective role in sweetpotato seedlings during osmotic stress.

Effect of pretreatment with hydrogen sulfide donor sodium hydrosulfide on heat tolerance in relation to antioxidant system in maize (Zea mays) seedlings

“…In present study, pretreatment with NaHS enhanced survival percentage of 2.5-dayold maize seedlings in a concentration-dependent manner (Fig. 1), consistent with the results of Zhang et al (2009bZhang et al ( , 2010a in wheat and sweetpotato under Cr and osmotic stress. All of above-mentioned studies indicated that H 2 S may be relative to the acquisition of stress tolerance including heat tolerance in plants, but their mechanisms are not fully clear.…”

“…Exposure of alfalfa (Medicago sativa) to 100 mM NaCl, seed germination and thereafter seedling growth were inhibited, but this negative effects could significantly be attenuated by NaHS at 100 µM . Zhang et al (2009b) reported that osmotic stress mimicked by PEG-6000 decreased dramatically chlorophyll in seedling leaves of sweetpotato (Ipomoea batata), and the osmotic-induced decrease in chlorophyll concentration was alleviated by spraying NaHS in a dose-dependent manner, similar to the reports of Zhang et al (2010b) in wheat seedlings. Also, In bermudagrass, exogenous application of NaHS improved salt (150 and 300 mM NaCl), osmotic (15% and 30% PEG6000) and cold (4 • C) tolerances, which were evidenced by decreased electrolyte leakage and increased survival rate under stress conditions (Shi et al 2013).…”

“…In plant systems, hydrogen sulfide has been found to play multiple physiological roles in plant growth, development and the acquisition of stress tolerance like heat tolerance (Lisjak et al 2010(Lisjak et al , 2013Li 2013). The novel functions of hydrogen sulfide have been uncovered in seed germination (Zhang et al 2010a;Li et al 2012a), root organogenesis (Zhang et al 2009a) and stomata movement (Lisjak et al 2010;Liu et al 2011;Jin et al 2013), as well as in alleviation in osmotic stress (Zhang et al 2009b), salt stress (Wang et al 2012), oxidative stress (Shan et al 2011, heavy metal stress (Zhang et al 2008(Zhang et al , 2010bChen et al 2013) and pathogen infection (Bloem et al 2011). Our previous results also showed that pretreatment with sodium hydrosulfide (NaHS), a hydrogen sulfide donor, could improve resistance of tobacco cells (Li et al 2012b) and maize seedlings (Li et al 2013a(Li et al ,b, 2014 to high temperature, but its mechanism is not fully clear.…”

The effect of flow turbulence on growth, nutrient uptake and stable carbon and nitrogen isotope signatures inChara fibrosa

“…Fresh plant samples were extracted in 100 mM phosphate buffer (Zhang et al, 2009) and the supernatant was used for the APX activity and CAT activity assays (Parida and Jha, 2010). The results are presented in mmol H 2 O 2 destroyed per minute per g of fresh weight (mmol H 2 O 2 min x1 g FW x1 ).…”

Hydrogen sulfide stimulates β-amylase activity during early stages of wheat grain germination

“…For the gel in (c), homogentes were incubated with 25 mm edtA to inactivate α-amylase and for (d), they were heated at 70°c to inactivate β-amylase. SO 2 injury, 10,20 and abiotic stresses tolerance in plants treated with H 2 S donor, 1,[21][22][23][24] all infer that H 2 S is involved in these responses.…”