In vitro antioxidant potential and in vivo effects of Schinus terebinthifolia Raddi leaf extract in diabetic rats and determination of chemical composition by HPLC-ESI-MS/MS

Abstract

The present study investigated the in vitro and in vivo antioxidant potential and phytochemical composition of Schinus terebinthifolia, which is widely used in folk medicine for various therapeutic purposes. The in vitro analyses indicated that the hydroethanolic extract (HE) had 312.50 ± 0.50 mg GAE/g of total phenols. It also presented anti-DPPH• and anti-ABTS• activity, reduced phosphomolybden and metal ions and blocked the bleaching of β-carotene. The HE at concentrations of 3.0 and 2.0 μg/mL had TRAP values of 2.223 ± 0.018 and 1.894 ± 0.026 μM Trolox, respectively. The HE increased the availability of antioxidants in plasma in treated animals in vivo. HPLC-ESI-MS/MS indicated the presence of 11 phenols: cumaric acid, (+)-catechin, myricetin-3-O-glicuronide, kaempferol-3-O-glucoside, myricetin, myricitrin, quercetin, gallic acid, methyl galate, pentagalloyl glucose and ethyl galate. Thus, S. terebinthifolia has potential for the prevention or treatment of diseases that are related to oxidative stress, such as diabetes mellitus.

Antioxidant activity and antidiabetic activities of Northern Thai indigenous edible plant extracts and their phytochemical constituents

“…Decne ( Srinuanchai et al., 2021 ), Aegle marmelos (L.) Corrêa ( Sharma et al., 2007 ), B. alba L. ( Bamidele et al., 2014 ; Ahmed, 2022 ), Glinus oppositifolius (L.) Aug.DC ( Behera et al., 2010 ), Amaranthus lividus Linn. ( Sangameswaran and Jayakar, 2008 ), Dregea volubilis Benth ( Thuy et al., 2021 ; Natarajan and Dhas, 2013 ; Natarajan et al., 2020 ), S. terebinthifolius Raddi ( da Rocha et al., 2019 ; Iwanaga et al., 2019 ), Tiliacora triandra (Colebr.) Diels ( Katisart and Rattana, 2017 ; Makinde et al., 2020 ).…”

extract of Schinus terebinthifolius Raddi intensifies morphological alterations in the colon of diabetic rats

“…Currently, there are more than 13 million diabetics aged 20 to 79 years and 88,300 children and adolescents in Brazil. 1,2 In addition the classic symptoms of chronic hyperglycemia, which leads to an imbalance of antioxidants, [3][4][5] diabetics suffer from complications in the gastrointestinal tract. 6 Dysphagia, abdominal pain, diarrhea, and constipation are symptoms that affect the quality of life of diabetic patients.…”

Chemical and cytotoxicity profiles of 11 pink pepper (Schinus spp.) samples via non-targeted hyphenated high-performance thin-layer chromatography

“…The bark and fruits are used to treat urinary and uterine affections, bleeding and diarrhea, and rheumatic and respiratory diseases. Antioxidant (Bendaoud et al, 2010 ; Feriani et al, 2021 ; Kim et al, 2021 ; Oliveira et al, 2020b ), antibacterial (D'Sousa' Costa et al, 2015 ; Linden et al, 2020 ; Salem et al, 2018 ), antifungal (Giordani et al, 2022 ), antidiabetic (Dos Santos da Rocha et al 2019 ; Iwanaga et al, 2019 ), and other bioactivities have been reported. Anti-inflammatory properties, for example, have been linked to the composition of polyphenols, as well as monoterpenes and sesquiterpenes in the essential oil (Feriani et al, 2020 ; Formagio et al, 2011 ; Kim et al, 2021 ; Marangoni et al, 2022 ; Rosas et al, 2015 ; Yueqin et al, 2003 ).…”