In vivo and in vitro activities of the seed extract of Piper guineense Schum. and Thonn. against skin and gill monogenean parasites of goldﬁsh (Carassius auratus auratus)

Ekanem, A. P.; Wang, M.; Simon, James E.; Obiekezie, A. I.; Morah, Frank Ni

doi:10.1002/ptr.1550

Cited by 40 publications

(19 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this reason, it is necessary to test those plant extracts which could be expected to contain substances of environmental potential, and at the same time, provide adequate efficacy against Dactylogyrus. Earlier authors reported that the methanolic extracts of the seeds of Piper guineense (Piperaceae) were active against skin and gill monogenean parasites of goldfish under in vivo and in vitro conditions (Ekanem et al 2004). In our previous work, chloroform extract of Fructus arctii (dried fruits of Arctium lappa L.) exhibited a 100% anthelmintic efficacy against D. intermedius at the lowest concentration of 240.0 mg/L after 48 h of exposure (Wang et al 2009).…”

Section: Discussionmentioning

confidence: 98%

In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Liu

Wang

et al. 2010

Parasitol Res

View full text Add to dashboard Cite

In search of a natural antiparasitic, in vivo anthelmintic activity of petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts of Angelica pubescens roots (Radix angelicae pubescentis), Brucea javanica fruits (Fructus bruceae), Spatholobus suberectus stems (Caulis spatholobi), Aesculus chinensis Bge. seeds (Semen aesculi), and Pharbitis purpurea (L.) Voigt seeds (Semen pharbitidis) were tested against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Among the extracts tested, the methanolic and aqueous extracts of S. aesculi were observed to be more efficient than the other plant extracts with EC(50) and EC(90) values of 5.23 and 7.33 mg/L and 6.48 and 12.29 mg/L after 48 h, respectively, followed by methanolic extracts of Fructus bruceae, Radix angelicae pubescentis, Caulis spatholobi, and Semen pharbitidis with EC(50) 49.96, 57.45, 64.92, and 309.47 mg/L. The methanolic and aqueous extracts of S. aesculi exhibited potential results and can be exploited as a preferred natural antiparasitic for the control of D. intermedius.

show abstract

Section: Discussionmentioning

confidence: 98%

In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Liu

Wang

et al. 2010

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…There is growing interest in using plant extracts for the treatment of human and animal disease, but little information is available on the use of the plant-derived products in the treatment of aquaculture diseases (Ekanem et al 2004). In the present study, we made a screen test using the in vivo anthelmintic efficacy assay, resulting in the selection of the Fructus Arctii from many medical plants.…”

Section: Discussionmentioning

confidence: 99%

“…Current research efforts focused on developing alternative drug formulations including medical plants. The methanol extracts of Piper guineense seeds (Piperaceae) were effective to monogenean parasites (Ekanem et al 2004). Two compounds, osthol and isopimpinellin, from Frutus cnidii have been reported to have the anthelmintic efficacy against Dactylogyrus intermedius (Wang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Bioassay-guided isolation and identification of active compounds from Fructus Arctii against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

et al. 2009

View full text Add to dashboard Cite

Dactylogyrus intermedius is a significant monogenean parasite on the gills of cyprinid fishes and can cause serious problem in fish aquaculture. In the present study, bioassay-guided fractionation was employed to identify the active compounds from Fructus Arctii against D. intermedius. Five solvents (petroleum ether, chloroform, ethyl acetate, ethanol, and water) were applied for the extraction of Fructus Arctii. Among them, only the chloroform extract exhibited promising anthelmintic efficacy and therefore, subjected to the further isolation and purification using various chromatographic techniques. Two compounds showing potent activity were obtained and identified by spectral data (infrared, nuclear magnetic resonance, and mass spectrometry) as: arctigenin (1) and arctiin (2). They were found to be significantly effective against D. intermedius with median effective concentration (EC(50)) values of 0.62 and 3.55 mg L(-1), respectively. Arctigenin exhibited higher activity as compared with the positive control mebendazole with an EC(50) value of 1.25 mg L(-1). The 48-h acute toxicity tests (LC(50)) of arctigenin and arctiin were found to be 8.47 and 14.14 mg L(-1) for goldfish, respectively. These results provided evidence that the studied plant extract, as well as the isolated compounds, might be potential sources of new antiparasitic drug for the control of Dactylogyrus.

show abstract

“…Current research efforts are increasingly focused on developing alternative drug formulations including medical plants. Ekanem (Ekanem et al 2004) found that the methanol extracts of the seeds of Piper guineense (Piperaceae) were active against Dactylogyrus. Crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis were also found to exhibit a complete elimination of all D. intermedius in goldfish (Liu et al 2010).…”

Section: Introductionmentioning

confidence: 98%

Activity of the chelerythrine, a quaternary benzo[c]phenanthridine alkaloid from Chelidonium majus L. on Dactylogyrus intermedius

Yao

Zhang

et al. 2011

Parasitol Res

View full text Add to dashboard Cite

Dactylogyrus intermedius is a significant monogenean parasite on the gills of cyprinid fishes and can cause severe economic losses in aquaculture and ornamental fish breeding. In the present study, bioactivity-guide fractionation was employed to identify active compound from Chelidonium majus L. against D. intermedius. In vivo anthelmintic activity of petroleum ether, ethyl acetate, chloroform, and n-butanol extracts of C. majus were tested. Among them, only the n-butanol extract exhibited promising anthelmintic efficacy, and therefore subjected to the further isolation and purification using various chromatographic techniques. A compound showing potent activity was obtained and identified by hydrogen, carbon-13 nuclear magnetic resonance spectrum and electron ionization mass spectrometry as chelerythrine. In vivo anthelmintic efficacy tests exhibited that chelerythrine was 100% effective against D. intermedius at a concentration of 1.60 mg L(-1), with LC(50) values of 0.68 mg L(-1) after 48 h of exposure. The 48-h LC(50) value (acute toxicity tests) of chelerythrine was found to be 3.59 mg L(-1) for grass carp. These results provided evidence that chelerythrine can be selected as a lead compound for the development of new drugs against D. intermedius.

show abstract

In vivo and in vitro activities of the seed extract of Piper guineense Schum. and Thonn. against skin and gill monogenean parasites of goldﬁsh (Carassius auratus auratus)

Cited by 40 publications

References 10 publications

In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Bioassay-guided isolation and identification of active compounds from Fructus Arctii against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Activity of the chelerythrine, a quaternary benzo[c]phenanthridine alkaloid from Chelidonium majus L. on Dactylogyrus intermedius

Contact Info

Product

Resources

About