<i>In vivo</i> evaluation of bioactive glass‐based coatings on dental implants in a dog implantation model

Oirschot, Bart A. J. A. van; Alghamdi, Hamdan S.; Närhi, Timo; Anil, Sukumaran; Aldosari, Abdullah Al Farraj; Beucken, Jeroen J.J.P. van den; Jansen, John A.

doi:10.1111/clr.12060

Cited by 34 publications

(33 citation statements)

References 37 publications

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“…Autoclaving was often used in other dicalcium silicate coating researches [38,39]. This is a common disinfection method for coating implant in vitro or in vivo studies [40,41].…”

Section: Discussionmentioning

confidence: 99%

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca2SiO4, C2S) particles. However, the immunological effects of C2S coatings have not been studied. C2S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C2S particles. Further, it remains unclear whether C2S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C2S coating on RAW 264.7 macrophages. C2S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C2S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested coating materials are not cytotoxic to macrophages. The C2S-coated surface stimulated macrophages to express pro-inflammatory mediators, such as TNF-α, IL-6 and IL-1β, and C2S coating caused less IL-6 but greater IL-1β production than the 45S5 coating. C2S coating have no cytotoxicity when directly cultured with macrophages. C2S and 45S5 coatings both have the potential to induce pro-inflammatory effects, and the biocompatibility of C2S is similar to that of 45S5.

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“…Autoclaving was often used in other dicalcium silicate coating researches [38,39]. This is a common disinfection method for coating implant in vitro or in vivo studies [40,41].…”

Section: Discussionmentioning

confidence: 99%

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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“…The first study assaying the deposition of bioactive SBG layers by RF-MS has been published only in 2003 by Mardare et al [358]. Following this peripheral attempt, there can be identified in the main scientific data bases (e.g., Web of Science ® , Scopus ® , Pubmed ® ) significant research efforts devoted by the groups led by Jansen and van den Beucken [359,361,370,371,372], on SBG—hydroxyapatite mixed layers, and Stan and Ferreira [151,304,307,308,309,310,311,312,313,362,363,373,374], (on pure SBG thin films, with only isolated trials published by Slav et al [360] and Saino et al [364]. Recently, Stuart et al [375,376,377,378,379] debuted a series of studies on the preparation by RF-MS of resorbable phosphate bioglass (PBG) thin films that are capable of releasing various therapeutic (e.g., antimicrobial) ions in a fast, but controllable manner.…”

Section: Bioactive Glasses and Glass-ceramicsmentioning

confidence: 99%

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

et al. 2018

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The discovery of bioactive glasses (BGs) in the late 1960s by Larry Hench et al. was driven by the need for implant materials with an ability to bond to living tissues, which were intended to replace inert metal and plastic implants that were not well tolerated by the body. Among a number of tested compositions, the one that later became designated by the well-known trademark of 45S5 Bioglass® excelled in its ability to bond to bone and soft tissues. Bonding to living tissues was mediated through the formation of an interfacial bone-like hydroxyapatite layer when the bioglass was put in contact with biological fluids in vivo. This feature represented a remarkable milestone, and has inspired many other investigations aiming at further exploring the in vitro and in vivo performances of this and other related BG compositions. This paradigmatic example of a target-oriented research is certainly one of the most valuable contributions that one can learn from Larry Hench. Such a goal-oriented approach needs to be continuously stimulated, aiming at finding out better performing materials to overcome the limitations of the existing ones, including the 45S5 Bioglass®. Its well-known that its main limitations include: (i) the high pH environment that is created by its high sodium content could turn it cytotoxic; (ii) and the poor sintering ability makes the fabrication of porous three-dimensional (3D) scaffolds difficult. All of these relevant features strongly depend on a number of interrelated factors that need to be well compromised. The selected chemical composition strongly determines the glass structure, the biocompatibility, the degradation rate, and the ease of processing (scaffolds fabrication and sintering). This manuscript presents a first general appraisal of the scientific output in the interrelated areas of bioactive glasses and glass-ceramics, scaffolds, implant coatings, and tissue engineering. Then, it gives an overview of the critical issues that need to be considered when developing bioactive glasses for healthcare applications. The aim is to provide knowledge-based tools towards guiding young researchers in the design of new bioactive glass compositions, taking into account the desired functional properties.

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“…This makes BG an attractive option for metal implant coating, reducing the potential for stress shielding and subsequent bone resorption which occurs with decreased bone loading [24]. To date, studies using dental implants coated with BGs have not conclusively shown significant increases in osseointegration relative to other coatings such as HA [25]. However, novel exploratory combinations for dental implant coatings using BG are promising: one example is the incorporation of BGs with HA and the polymer poly(lactide- co -glycolide) (PLGA), which indicates rapid bone-like apatite formation in vitro, with potential antimicrobial activity on oral bacteria [26].…”

Section: Scaffoldsmentioning

confidence: 99%

Tissue engineering for bone regeneration and osseointegration in the oral cavity

et al. 2015

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In vivo evaluation of bioactive glass‐based coatings on dental implants in a dog implantation model

Cited by 34 publications

References 37 publications

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

Tissue engineering for bone regeneration and osseointegration in the oral cavity

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