In vivofluorescence imaging for tissue diagnostics

Abstract

In vivo fluorescence imaging for tissue diagnosticsAndersson-Engels, Stefan; af Klinteberg, C; Svanberg, Katarina; Svanberg, Sune General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. Non-invasive fluorescence imaging has the potential to provide in vivo diagnostic information for many clinical specialities. Techniques have been developed over the years for simple ocular observations following UV excitation to sophisticated spectroscopic imaging using advanced equipment. Much of the impetus for research on fluorescence imaging for tissue diagnostics has come from parallel developments in photodynamic therapy of malignant lesions with fluorescent photosensitizers. However, the fluorescence of endogenous molecules (tissue autofluorescence) also plays an important role in most applications. In this paper, the possibilities of imaging tissues using fluorescence spectroscopy as a mean of tissue characterization are discussed. The various imaging techniques for extracting diagnostic information suggested in the literature are reviewed. The development of exogenous fluorophores for this purpose is also presented. Finally, the present status of clinical evaluation and future directions are discussed. In vivo fluorescence imaging for tissue diagnostics

Imaging Tri-Fusion Multimodality Reporter Gene Expression in Living Subjects

“…Use of light is probably the oldest method of analyzing tissues in biomedical science (25). The various optical imaging approaches including fluorescence microscopy (at the cellular level), diffuse optical tomography, and intravital microscopy (for deeper structures at the organism level) are commonly used (26,27).…”

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

“…Sensitizers, including porphyrins, phtalocyanines and chlorines, exhibit characteristic fluorescence signatures in the near infrared spectrum that can serve for clinical tumor diagnostics. 8,15 By irradiation of red light the same compounds can also mediate tumor cell necrosis through selective singlet oxygen release ͑photodynamic therapy 19 ͒. A particularly interesting sensitizer is protoporphyrin IX, which is synthesized at a higher rate in malignant tumors after administration of ␦-amino levulinic acid.…”

“…3,4 It can be applied in point monitoring 5 or in imaging applications. 6,7 Applications include diagnostics of human malignant tumors 8,9 and atherosclerotic disease, 10 vegetation and water monitoring, 11,12 analytical chemistry techniques such as HPLC and capillary electrophoresis, 13 and forensic sciences. 14 Fluorescence diagnostics has been largely simplified through the availability of CCD detectors for direct readout of the spectrum obtained in the image plane of a spectrometer.…”

Neodymium-doped nanoparticles for infrared fluorescence bioimaging: The role of the host

“…Molecular probes, such as fluorescent proteins and dyes, have been extensively used for this purpose and some of them have been approved for clinical use. [12][13][14] Although they exhibit good brightness owing to high emission quantum yield, these probes present some drawbacks, including a high susceptibility to photobleaching or requirement to use short wavelength photoexcitation, which has motivated scientists to search for alternative optical probes. One of the most promising alternatives is based on luminescent nanoparticles (LNPs).…”