Lactobacillus plantarum AR501 Alleviates the Oxidative Stress of D‐Galactose‐Induced Aging Mice Liver by Upregulation of Nrf2‐Mediated Antioxidant Enzyme Expression

Abstract

Lactic acid bacteria (LAB) have been used as ingredients of functional foods to promote health and prevent diseases because of their beneficial effects. This study aimed to investigate the antioxidative effects of LAB on the hepatotoxicity in D-galactose-induced aging mice. LAB were isolated from the traditional Chinese fermented foods and screened by the tolerance of hydrogen peroxide (H O ). Male ICR (Institute of Cancer Research) mice were subcutaneously injected with D-galactose for 5 weeks and then gastric gavage with LAB for 6 weeks. The results showed that Lactobacillus plantarum AR113 and AR501, and Pediococcu pentosaceus AR243 could tolerate up to 1.5 mM H O in vitro, and they could live through simulated gastrointestinal tract (GIT) to colonizing the GIT of host. In vivo, oral administration of L. plantarum AR113 and AR501 improved the antioxidant status of D-galactose-induced oxidative stress mice such as alleviated liver damages and reduced abnormal activities of superoxide dismutase, glutathione peroxidase, and catalase to normal levels. In addition, L. plantarum AR501 markedly elevated the gene expression of nuclear factor erythroid-2-related factor 2 and upregulated the expressions of several antioxidant genes such as glutathione reductase, glutathione S-transferase, glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit, and NAD(P)H quinone oxidoreductase 1 in the liver of an aging mice. Therefore, L. plantarum AR501 could be a good candidate for producing antiaging functional foods.

Reversal of age-associated oxidative stress in mice by PFT, a novel kefir product

“…Recent studies showed that other probiotics have the ability in mice to improve biomarkers' expression related to aging and alleviate oxidative stress, including the probiotic Dahi (containing Bifidobacterium bifidum and Lactobacillus acidophilus) 51 and Lactobacillus plantarum AR113 and AR501. 52 Other LABs have also been shown to relieve oxidative stress in other models. 53,54 Furthermore, we have previously shown that MRN-100, an iron-based dietary supplement, can reverse age-related oxidative stress.…”

Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity

“…Generally, excess of free radicals is mainly eliminated by redox systems in the body, such as the sulfur oxygen reduction (TRX) system and the glutathione (GSH) system, which can reduce and control the occurrence of the oxidative stress-related diseases (Yu et al 2015;Lin et al 2018a). GSH-Px is the most important antioxidant factor in the glutathione (GSH) system, which could directly scavenge free radicals and prevent cell damage (Esposito et al 2000;Ding et al 2017).…”

Lactic Acid Bacteria With Antioxidant Activities Alleviating Oxidized Oil Induced Hepatic Injury in Mice

“…L. plantarum AR113, P. pentosaceus AR243, L. plantarum AR269, L. plantarum AR300, and L. plantarum AR501 were used in this study. They were previously screened by tolerance of hydrogen peroxide up to 1.5 mM ( Lin et al, 2018 ). All strains were isolated on de Man, Rogosa, and Sharpe (MRS) agar plates and stored in MRS broth with 20% glycerol at −80°C.…”