Identification and determination of compounds belonging to the group of OUN pharmaceutical agents by thin-layer chromatography with densitometric detection in biological material

Abstract

The chromatographic-densitometric method was developed for the simultaneous determination of various pharmaceutical agents, such as haloperidol, fluxetine, promazine, doxepin, clonazepam, alprazolam, and risperidone in biological material. Two mobile phases, A (acetone-diethylamine-cyclohexane (2.5:2.5:20, v/v/v)) and B (trichloromethane-acetone-25% NH 3 (25:25:0.5, v/v/v)), were used for the separation of the above-mentioned compounds. Silica gel-covered F 254 TLC plates were used as stationary phases. Densitometric recording was carried out at 254 nm wavelength. Welldeveloped peaks, easy to quantify, and quality analysis were obtained after achieving the separation of compounds in the developed conditions. It was demonstrated that the presence of a matrix (plasma or urine) did not affect the shape of the peaks and their localization on the chromatograms.

Guava-fruit based synthesis of carbon quantum dots for spectrofluorometric quantitative analysis of risperidone in spiked human plasma and pharmaceutical dosage forms

“…Several techniques for determining ARP in various matrices have been published in the literature, including chromatographic, [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] electrochemical, [31][32][33][34][35][36][37][38] and spectroscopic [39][40][41][42][43][44] methods. These techniques have signicant drawbacks, such as being time-consuming, requiring sample pretreatment before measurement, being costly and unavailable in small laboratories, providing poor sensitivity, and not adhering to green analytical chemistry principles.…”

Determination of fluoxetine in the presence of photodegradation products appearing during UVA irradiation in a solid phase by chromatographic-densitometric method, kinetics and identification of photoproducts

“…Liquid chromatography with MS detection [10][11][12][13][14], gas chromatography (GC)-MS [15][16][17][18], and capillary electrophoresis [19] were mainly used for the determination of fluoxetine in biological samples. Fluoxetine was also determined in pharmaceutical preparations [7,[20][21][22][23] and in biological material [24] by thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC). In addition to separation techniques, the following methods were used: spectrophotometry [25][26][27], derivative spectrophotometry [28], spectrofluorimetry [29,30], potentiometry [31], voltamperometry [32], and conductometry [33].…”