Identification, isolation and characterization of a CC-NBS-LRR candidate disease resistance gene family in grapevine

Kortekamp, A.; Welter, Leocir José; Vogt, Sarah J.; Knoll, Alexander; Schwander, Florian; Töpfer, Reinhard; Zyprian, Eva

doi:10.1007/s11032-008-9186-2

“…Further, ccnBS-lRR candidate resistance gene family, expressed after inoculation with P. viticola, was isolated, characterized and mapped on lG 10 of the cultivar Regent resistant to powdery and downy mildew (23).…”

Section: Identification Of Qtlsmentioning

confidence: 99%

“…the established genetic maps present a source for markers for other mapping projects and are useful tool for identification of QTLs/markers, for anchoring BAC clones, and for map-based cloning of candidate genes related to disease resistance. QTL analysis is a widely applied approach for identification of loci underlying disease resistance in the frame of genetic linkage map, but it is not effective enough for identification of loci with a minor effect concerning pathogen resistance (23). through the use of this approach, one major and one minor qtl with a strong effect on the resistance to downy mildew were identified and mapped on LG (linkage group) 18 and lG 4, respectively in the integrated "Regent" x "lemberger" genetic map.…”

Section: Introductionmentioning

confidence: 99%

Toward Marker Assisted Selection for Fungal Disease Resistance in Grapevine

Hvarleva¹,

Bakalova²,

Rusanov³

et al. 2009

Biotechnology & Biotechnological Equipment

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“…Although cultivated grapevines (V. vinifera L.) are highly susceptible to several diseases, some wild cultivars such as V. riparia, V. rupestris, V. rotundifolia and V. flexuosa are reportedly resistant to several important grapevine diseases (Eibach et al 1989;Kortekamp et al 2008;Ahn et al 2012). In a previous study, we found several transcripts of EDS1-like genes with differential expression levels in the transcriptome of V. flexuosa infected with E. ampelina (Ahn et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Three transcripts of EDS1-like genes respond differently to Vitis flexuosa infection

Islam

¹

,

Yun

²

2017

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/ separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobium vitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

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“…Because the application of commercial fungicides to control diseases is very expensive and causes various adverse effects on the environment, interest in the development of disease resistant grape cultivars is increasing. While cultivated grape species (Vitis vinifera L.) are highly susceptible to many diseases (Wang et al 2011), wild cultivars such as V. riparia, V. rupestris, and V. rotundifolia are resistant to several important grapevine diseases (Eibach et al 1989;Kortekamp et al 2008;Reisch et al 2012). Among Vitis species native to Korea, the wild cultivar V. flexuosa has been reported to show resistant responses such as expression of defense related genes against pathogen attacks (Ahn et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Expression of Genes Related with Defense Responses against Pathogen Infections in Vitis flexuosa

Islam¹,

Ahn²,

Kim³

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Quantitative real-time PCR revealed that R and R-related genes in V. flexuosa induced defense responses through expression of genes in SA-pathway rather than in the JA-pathway, and R-related genes were more closely correlated with lignin and phytoalexin biosynthesis than anthocyanin biosynthesis against different pathogen infections in V. flexuosa. Specifically, genes such as VfRLK586, VfRLK2422, VfRLK5099, VfRLK29610, VfRLK55257, VfRPS5-like4135, VfRPS5-like4832, VfRPS5-like20585, VfRPS5-like55532, VfEDL2, VfEDL3, VfCXE5585, VfCXE12827, and VfCXE13132 showed close correlation with induction of genes in the SA-mediated pathway, lignin, phytoalexin, and anthocyanin biosynthesis. Genes including VfRLK2422, VfRLK5099, VfRLK29610, VfRPS5-like55532, VfEDL3, and VfCXE12827 were also correlated with the JA-mediated signaling pathway in induction of defense responses.

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Identification, isolation and characterization of a CC-NBS-LRR candidate disease resistance gene family in grapevine

Cited by 44 publications

References 30 publications

Toward Marker Assisted Selection for Fungal Disease Resistance in Grapevine

Toward Marker Assisted Selection for Fungal Disease Resistance in Grapevine

Three transcripts of EDS1-like genes respond differently to Vitis flexuosa infection

Expression of Genes Related with Defense Responses against Pathogen Infections in Vitis flexuosa

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