2003
DOI: 10.1128/jcm.41.2.547-551.2003
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Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

Abstract: The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzymelinked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported previously (H. Hirata et al., J. Clin. Microbiol. 40:1470-1474, 2002). However, the ELISA with the GST/Be82 antigen cross-reacted with Babesia caballi-infected horse sera, despite the high rate of detection of B. equi. These results suggested that GST/Be82 has an antigen in common with B. caballi or antigenicity similar to … Show more

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Cited by 15 publications
(11 citation statements)
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“…equi (TANAKA et al, 1999). More recently, the identification of a specific antigenic region of the Be82 gene allowed the development of a novel ELISA with an antigen (GST/Be82/236-381) that proved to have specificity and sensitivity for B. equi antibodies of 100% (HIRATA et al, 2003). However, the use of recombinant antigens in the diagnosis of equine piroplasmosis has a high cost when compared to crude antigenic preparations.…”
Section: Discussionmentioning
confidence: 99%
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“…equi (TANAKA et al, 1999). More recently, the identification of a specific antigenic region of the Be82 gene allowed the development of a novel ELISA with an antigen (GST/Be82/236-381) that proved to have specificity and sensitivity for B. equi antibodies of 100% (HIRATA et al, 2003). However, the use of recombinant antigens in the diagnosis of equine piroplasmosis has a high cost when compared to crude antigenic preparations.…”
Section: Discussionmentioning
confidence: 99%
“…HEUCHERT et al (1999) tested 752 serum samples from São Paulo State by IFAT and CFT and reported prevalence rates of 29.6% and 17.6%, respectively. Serum samples from horses in different regions of Brazil were tested by TFC in order to determine the prevalence of equine babesiosis, which was 42.48% for B. equi and 33.77% for B. caballi (KERBER et al, 1999). Recently, XUAN et al (2001) recorded prevalence of 81% for B. equi infection in the States of São Paulo and Mato Grosso do Sul, Brazil.…”
Section: Discussionmentioning
confidence: 99%
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“…Although IFAT is more sensitive than CFT and rarely renders false negative results FRIEDHOFF, 1986), standardization is difficult, considering the subjectivity of the reader in assessing the results (BÖSE et al, 1995;BRÃœNING, 1996). ELISA is nowadays an alternative for increased specificity and sensitivity in the detection of acute and latent babesial infections and several procedures of ELISA using recombinant antigen or crude antigen have been standardized (BALDANI et al, 2007;KUMAR et al, 2003;HIRATA et al, 2003;XUAN et al, 2001;KATZ et al, 2000).…”
Section: Introductionmentioning
confidence: 99%
“…Besides the CFT and IFAT, the enzyme-linked immunosorbent assay (ELISA) has also been used for the detection of Theileria infection. Various researchers have suggested this serological test as an alternative for increased specificity and sensitive detection of acute and latent infections, especially with the use of recombinant antigens (Tanaka et al, 1999;Xuan et al, 2001ab;Hirata et al, 2003).…”
Section: Introductionmentioning
confidence: 99%