Identification of anti-HIV and anti-Reverse Transcriptase activity from Tetracera scandens

Kwon, Hyeok; Park, Jung Ae; Kim, Joo Hwan; You, Ji Chang

doi:10.5483/bmbrep.2012.45.3.165

Cited by 12 publications

(9 citation statements)

References 19 publications

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“…Following these two steps, the host cell produces viral proteins and genomic material using proteases. Studies with natural products with anti-HIV activity focus on anti-retroviral drugs that inhibit the activity of these three types of enzymes: RT, proteases and integrases (Kwon et al, 2012;Suedee et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…This activity is related to the presence of calanolides, but the plant source is relatively rare. Kwon et al (2012), studying Tetracera scandens (Dilleniaceae) extracts, estimated an IC 50 level of 0.7 μg mL -1 against in vitro HIV-1 RT. According to García-Zebadúa et al (2011), considering the HIV-1 RT inhibitory properties of plant extracts, some authors have proposed that an IC 50 ≤ 50 μg mL -1 may be considered potent.…”

Section: Resultsmentioning

confidence: 99%

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Alkaloids from Croton echinocarpus Baill.: Anti-HIV potential

Ravanelli

Santos

Motta

et al. 2016

South African Journal of Botany

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Croton belongs to the Euphorbiaceae genus, one of the major Angiosperms families, and is widely distributed throughout the world, especially in the tropics. In this study, the hydroalcoholic extract (70% EtOH) of Croton echinocarpus leaves afforded two alkaloids: corydine and norisoboldine, and their structures were established by spectroscopic data interpretation (UV, IR, NMR and LREIMS). Both alkaloids displayed significant in vitro anti-HIV potential, inhibiting 40% of the HIV-1 reverse transcriptase enzyme activity at a concentration of 100 μg mL -1 of norisoboldine and 450 μg mL -1 of corydine. Corydine showed IC 50 of 356.8 μg mL -1 , while norisoboldine was more efficient on inhibiting the RT activity, showing IC 50 of 153.7 μg mL -1 .

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Alkaloids from Croton echinocarpus Baill.: Anti-HIV potential

Ravanelli

Santos

Motta

et al. 2016

South African Journal of Botany

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“…Relative amounts of LDH secreted to the medium was calculated as LDH present in media/(LDH present in media + LDH present in cell pellet) and compared to that of the control group which was treated with DMSO. An MTT assay was performed as described (30).…”

Section: Methodsmentioning

confidence: 99%

Dexamethasone induces the expression of LRRK2 and α-synuclein, two genes that when mutated cause Parkinson's disease in an autosomal dominant manner

Park

Yun³

et al. 2013

BMB Reports

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LRRK2 (leucine-rich repeat kinase 2) has been identified as a gene corresponding to PARK8, an autosomal-dominant gene for familial Parkinson’s disease (PD). LRRK2 pathogenicspecific mutants induce neurotoxicity and shorten neurites. To elucidate the mechanism underlying LRRK2 expression, we constructed the LRRK2-promoter-luciferase reporter and used it for promoter analysis. We found that the glucocorticoid receptor (GR) transactivated LRRK2 in a ligand-dependent manner. Using quantitative RT-PCR and Western analysis, we further showed that treatment with dexamethasone, a synthetic GR ligand, induced LRRK2 expression at both the transcriptional and translational levels, in dopaminergic MN9D cells. Dexamethasone treatment also increased expression of α-synuclein, another PD causative gene, and enhanced transactivation of the α-synuclein promoter-luciferase reporter. In addition, dexamethasone treatment to MN9D cells weakly induced cytotoxicity based on an LDH assay. Because glucocorticoid hormones are secreted in response to stress, our data suggest that stress might be a related factor in the pathogenesis of PD. [BMB Reports 2013; 46(9): 454-459]

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“…The ethanol extract of T. scandens has anti-HIV activity and high inhibitory activity against HIV-1 reverse transcriptase activity [5]. The methanol extract of T. Scandens stem yields new nor-lupane triterpene capable of exhibiting significant concentration-dependent xanthine oxidase inhibitory activity [1].…”

Section: Introductionmentioning

confidence: 99%

Tetracera scandens as a Medicinal Plant: Secretory Structures, Histochemistry, and Antibacterial Activity

Muliyah

Sulistijorini

Sulistyaningsih

et al. 2018

J.Trop.Life.Science

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Tetrascera scandens, a member of Dilleniaceae, is used for traditional medicine; the stem is utilized by the Anak Dalam tribe of Jambi Province, Sumatera island, Indonesia, to treat diarrhea symptoms. The aims of this study were to identify the secretory structures, histochemical aspects, and the antibacterial potency of T. scandens stem. Histological study of the secretory structures of T. scandens stem was carried out. The species has idioblast cells and trichomes as its secretory structures. Histochemical analysis indicated the substance secreted by T. scandens idioblast cells mainly contains alkaloids, terpenoids, and phenols. Trichomes of T. scandens only contain flavonoids. The antibacterial activity of methanol extracts was tested against Staphyllococcus aureus and Escherichia coli. Different concentration of extracts was tested using the well diffusion method. According to the results, 100 mg/mL T. scandens extract showed the best inhibitory activity with a maximum inhibition zone of 17.7 mm against S. aureus and of 12.5 mm against E. coli. This study provides scientific evidence that the stem of T. scandens has antibacterial activity and justifies its use by the local community.

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Identification of anti-HIV and anti-Reverse Transcriptase activity from Tetracera scandens

Cited by 12 publications

References 19 publications

Alkaloids from Croton echinocarpus Baill.: Anti-HIV potential

Alkaloids from Croton echinocarpus Baill.: Anti-HIV potential

Dexamethasone induces the expression of LRRK2 and α-synuclein, two genes that when mutated cause Parkinson's disease in an autosomal dominant manner

Tetracera scandens as a Medicinal Plant: Secretory Structures, Histochemistry, and Antibacterial Activity

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