Identification of grass carp haemorrhage virus as a new genogroup of aquareovirus

Abstract

Three aquareovirus strains isolated from grass carp (Ctenopharyngodon idellus), geoduck clams (Panope abrupta) and herring (Clupea harengus) in North America and Asia were examined by RNA-RNA blot hybridization to determine their genogroup. The isolates from clams and herring were identified as members of genogroup A, but the isolate from grass carp did not hybridize to any of the known genogroups, suggesting that this virus probably represents a new, seventh genogroup.

Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella

“…GCRV also infects black carp (Mylopharyngodon piceus), topmouth gudgeon (Pseudorasbora parva) and rare minnow (Gobiocypris rarus) [6,7]. Notably, GCRV was recognized to be the most virulent agent amongst all of the identified aquareovirus isolates in the genus Aquareovirus of the Reoviridae family [8]. In an attempt to control the spread of the disease, several inactive vaccines have been developed over the years.…”

An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus

“…Many methods have been used for the detection of GCRV or other aquareoviruses; these methods include antigen-related serological reactions (Ye et al, 1989;Yang et al, 1991;Dopazo et al, 1992;Shao et al, 1996), genome-related nucleic acid hybridization (Subramanian et al, 1993(Subramanian et al, , 1997Rangel et al, 1999) and classical morphological observations of viruses using electron microscopy (Winton et al, 1987). Additionally, reverse transcription-polymerase chain reaction (RT-PCR) techniques have also been developed (Li et al, 1997;Seng et al, 2004;Guo et al, 2008).…”

Sequence of Genome Segments 1, 2, and 3 of the Grass Carp Reovirus (Genus Aquareovirus, Family Reoviridae)

“…These viruses physically resemble orthoreoviruses and infect a variety of aquatic animals, including finfish and Crustacea (2). They are classified into six genogroups (A to F) and the genus includes a number of tentative species (3). Among unclassified species, figures the grass carp reovirus GCRV (or grass carp hemorrhage virus, GCHV) (2).…”