Identification of Microsatellite DNA Markers for Pacific Threadfin Parentage Assignment

Wang, Heng; Iwai, Thomas Y.; Zhao, Baoping; Lee, Cheng‐Sheng; Yang, Jinzeng

doi:10.1111/j.1749-7345.2010.00405.x

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…In a study of common carp ( Cyprinus carpio L.), the assignment success rate for 550 offspring from a full factorial cross of 10 dams × 24 sires with 8 microsatellite markers was 95.3% (Vandeputte et al, 2004). A total of 5 microsatellite loci were selected for a parentage assignment test of pacific threadfin ( Polydactylus sexfilis ), and 90% of the offspring from three consecutive spawning events were successfully assigned to their specific parent pairs (Wang, Iwai, Zhao, Lee, & Yang, 2010). Through the differences in the accuracy of parentage assignments above, we considered that, although a great many microsatellite loci can be easily obtained though genomic sequencing, some of them are not suitable for parentage assignment.…”

Section: Discussionmentioning

confidence: 99%

Genetic evidence for the mating system and reproductive success of black sea bream (Acanthopagrus schlegelii)

Wang

Liu²,

Yang³

et al. 2020

Ecology and Evolution

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Understanding the mating system and reproductive success of a species provides evidence for sexual selection. We examined the mating system and the reproductive success of captive adult black sea bream (Acanthopagrus schlegelii), using parentage assignment based on two microsatellites multiplex PCR systems, with 91.5% accuracy in a mixed family (29 sires, 25 dams, and 200 offspring). Based on the parentage result, we found that 93.1% of males and 100% of females participated in reproduction. A total of 79% of males and 92% of females mated with multiple partners (only 1 sire and 1 dam were monogamous), indicating that polygynandry best described the genetic mating system of black sea bream. For males, maximizing the reproductive success by multiple mating was accorded with the sexual selection theory while the material benefits hypothesis may contribute to explain the multiple mating for females. For both sexes, there was a significant correlation between mating success and reproductive success and the variance in reproductive success of males was higher than females. Variation in mating success is the greatest determinant to variation in reproductive success when the relationship is strongly positive. The opportunity for sexual selection of males was twice that of females, as well as the higher slope of the Bateman curve in males suggested that the intensity of intrasexual selection of males was higher than females. Thus, male-male competition would lead to the greater variation of mating success for males, which caused greater variation in reproductive success in males. The effective population number of breeders (N b ) was 33, and the N b /N ratio was 0.61, slightly higher than the general ratio in polygynandrous fish populations which possibly because most individuals mated and had offspring with a low variance. The relatively high N b contributes to the maintenance of genetic diversity in farmed black sea bream populations. K E Y W O R D SAcanthopagrus schlegelii, Bateman gradient, multiple matings, parentage analysis, polygynandry

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Section: Discussionmentioning

confidence: 99%

Genetic evidence for the mating system and reproductive success of black sea bream (Acanthopagrus schlegelii)

Wang

Liu²,

Yang³

et al. 2020

Ecology and Evolution

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“…In conclusion, genetic studies on Pacific threadfin with microsatellite DNA markers are very rare with only one report on the development of new microsatellites for parentage assignment available from our previous work 6. No detailed information is available hitherto on the genetic diversity of wild and cultured broodstocks of Pacific threadfin.…”

Section: Resultsmentioning

confidence: 98%

“…Currently, limited microsatellite DNA markers are available for Pacific threadfin 6. The present study is aimed at identifying new microsatellite loci and comparing the genetic similarity and differences of wild and hatchery Pacific threadfin populations in Hawaii.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Microsatellite DNA Markers Reveals no Genetic Differentiation between Wild and Hatchery Populations of Pacific Threadfin in Hawaii

Pan¹,

Yang²

2010

Int. J. Biol. Sci.

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Pacific threadfin, Polydactylus sexfilis, is popular fish in recreational fishing, as well as aquaculture in Hawaii. Its natural population has been continuously declining in the past several decades. Microsatellite DNA markers are useful DNA-based tool for monitoring Pacific threadfin populations. In this study, fifteen Microsatellite (MS) DNA markers were identified from a partial genomic Pacific threadfin DNA library enriched in CA repeats, and six highly-polymorphic microsatellite loci were employed to analyze genetic similarity and differences between the wild population and hatchery population in Oahu Island. A total of 37 alleles were detected at the six MS loci in the two populations. Statistical analysis of fixation index (FST) and analysis of molecular variance (AMOVA) showed no genetic differentiation between the wild and hatchery populations (FST=0.001, CI95%= -0.01-0.021). Both high genetic diversity (Ho=0.664-0.674 and He=0.710-0.715) and Hardy-Weinberg equilibrium were observed in the wild and hatchery populations. Results of genetic bottleneck analysis indicated that the hatchery was founded with sufficient numbers of brooders as inbreeding coefficient is very low (FIS=0.052-0.072) in both wild and hatchery populations. Further studies are needed for comprehensive determinations of genetic varieties of primary founder broodstocks and successive offspring of the hatchery and wild populations with increased number of Pacific threadfin sample collections.

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“…Thus, breeding strategies assisted by molecular DNA markers could be improved with detailed knowledge of the genetic composition of broodstock and offspring, minimizing inbreeding effects (Perez‐Enriquez, Takagi & Taniguchi ; Kim, Morishima, Satoh, Fujioka, Saito & Arai ). Selective breeding programs have been successfully applied to rainbow trout (Henryon, Berg, Olesen, Kjaer, Slierendrecht, Jokumsen & Lund ), common carp (Vandeputte ), sea bass (Garcia de Leon, Cannone, Quillet, Bonhomme & Chatain ; Costa, Vandeputte, Antonucci, Boglione, Menesatti, Cenadelli, Parati, Chavanne & Chatain ), Pacific threadfin (Wang, Iwai, Zhao, Lee & Yang ), oysters (Lallias, Boudry, Lapègue, King & Beaumont ), mussels (MacAvoy, Wood & Gardner ) and abalones (Kijima, Li & Park ; Vandeputte, Mauger & Dupont‐Nivet ; Hayes, Baranski, Goddard & Robinson ; Kube, Appleyard & Elliott ; Slabbert, Bester & D'Amato ).…”

Section: Introductionmentioning

confidence: 99%

Parentage assignment in hybrid abalones (Haliotis rufescens × Haliotis discus hannai) based on microsatellite DNA markers

2013

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Parentage analysis in aquaculture determines genealogical relationships between broodstock and progeny when the parents are unknown. Thus, parentage analysis is a useful tool to establish pedigree reports in molecular-assisted selection programs. Here, we evaluated 10 heterologous microsatellite markers for parentage assignment in abalone hybrids produced from 43 abalone broodstocks of red abalone (Haliotis rufescens) and Japanese abalone (H. discus hannai). The allele frequencies, exclusion probabilities and broodstock contributions were calculated using CERVUS, PAPA and GERUD software. The polymorphic information content (PIC) values showed that most of the microsatellite loci were highly informative (>0.7) and more than 90% of parentage assignment was possible with a minimum of 5-6 microsatellite markers. Parentage assignment for hybrid and pure-red progeny showed a better performance than pure-Japanese progeny. This result could be due to the high level of allele loss in the parental genotypes. In addition, results indicated that only two sires contributed over 80% and 90% of red and hybrid progenies, respectively. This study gives a new molecular tool to support marker-assisted selection in abalone hybrids produced in Chile.

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Identification of Microsatellite DNA Markers for Pacific Threadfin Parentage Assignment

Cited by 9 publications

References 14 publications

Genetic evidence for the mating system and reproductive success of black sea bream (Acanthopagrus schlegelii)

Genetic evidence for the mating system and reproductive success of black sea bream (Acanthopagrus schlegelii)

Analysis of Microsatellite DNA Markers Reveals no Genetic Differentiation between Wild and Hatchery Populations of Pacific Threadfin in Hawaii

Parentage assignment in hybrid abalones (Haliotis rufescens × Haliotis discus hannai) based on microsatellite DNA markers

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