Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical systems and species-specific PCR

Giordano, Alessandra; Magni, A; Trancassini, Maria; Varesi, Paola; Turner, Raymond J.; Mancini, Carlo

doi:10.1016/j.mimet.2005.04.020

Cited by 10 publications

(3 citation statements)

References 14 publications

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“…maltophilia clinical isolates from our collection were correctly identified by SS‐PCR. This result is in agreement with those obtained by Giordano et al. (2006) which confirmed the sensitivity of this PCR screening on strains isolated from CF and non‐CF patients.…”

Section: Discussionsupporting

confidence: 92%

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Identification of Stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure

Pinot

Deredjian

Nazaret

et al. 2011

Journal of Applied Microbiology

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Aims: Aim of the study is to identify accurately Stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. Methods and Results: Recovery of Sten. maltophilia‐like isolates from soil samples using the vancomycin, imipenem, amphotericin B (VIA) selective agar medium enabled distinction of various morphotype colonies. A set of soil and clinical isolates was tested for species identification using different methods. 16S rDNA analyses showed the dark green with a blue halo morphotype to be typical Sten. maltophilia strains. The API‐20NE, Vitek‐2 and Biolog phenotypic analyses typically used for the identification of clinical isolates did not perform well on these soil isolates. The species‐specific PCR screening targeting Sten. maltophilia 23S rDNA and the multiplex smeD/ggpS PCR, differentiating Sten. maltophilia from Stenotrophomonas rhizophila, were tested for improvement of these identification schemes. The latter multiplex PCR identified all isolates tested in this study, whatever be their origin. Conclusions: Isolation on VIA medium and confirmation of Sten. maltophilia species membership by smeD PCR is proposed to identify environmental and clinical isolates of Sten. maltophilia. Significance and Impact of the Study: The proposed approach enables isolation and identification of Sten. maltophilia from different environments in an easy and rapid way. This approach will be useful to accurately manage studies on the abundance and distribution of Sten. maltophilia in hospital and nonhospital environments.

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Section: Discussionsupporting

confidence: 92%

“…This PCR presented sensitivity and specificity of 100% for clinical strains or strains isolated from the environmental clinical area (Whitby et al. 2000; Giordano et al. 2006; Foster et al.…”

Section: Introductionmentioning

confidence: 99%

Identification of Stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure

Pinot

Deredjian

Nazaret

et al. 2011

Journal of Applied Microbiology

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“…The isolates were presumptively identified as S. maltophilia by Gram stain, catalase, oxidase, alpha hemolysis on blood agar, fermentation on MacConkey agar, DN ase production, and mannitol fermentation on mVIA agar medium. The isolates were further genotypically confirmed as S. maltophilia by single stranded PCR (SS-PCR) [18, 19]. Confirmed isolates were stored in Luria-Bertani (LB) broth (Merck Pvt.…”

Section: Methodsmentioning

confidence: 99%

In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain

Karunanidhi

Thomas

Belkum³

et al. 2013

BioMed Research International

101

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The in vitro antibacterial and antibiofilm activity of chlorogenic acid against clinical isolates of Stenotrophomonas maltophilia was investigated through disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill and biofilm assays. A total of 9 clinical S. maltophilia isolates including one isolate resistant to trimethoprim/sulfamethoxazole (TMP/SMX) were tested. The inhibition zone sizes for the isolates ranged from 17 to 29 mm, while the MIC and MBC values ranged from 8 to 16 μg mL−1 and 16 to 32 μg mL−1. Chlorogenic acid appeared to be strongly bactericidal at 4x MIC, with a 2-log reduction in viable bacteria at 10 h. In vitro antibiofilm testing showed a 4-fold reduction in biofilm viability at 4x MIC compared to 1x MIC values (0.085 < 0.397 A 490 nm) of chlorogenic acid. The data from this study support the notion that the chlorogenic acid has promising in vitro antibacterial and antibiofilm activities against S. maltophilia.

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Burkholderia , Stenotrophomonas , Ralstonia , Cupriavidus , Pandoraea , Brevundimonas , Comamonas , Delftia , and Acidovorax

Lipuma,

Currie,

Baird

et al. 2023

ClinMicroNow

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Many of the bacteria discussed in this chapter formerly belonged to the genus Pseudomonas; others were described and named after the taxonomic dissection of this genus to conform to a modern phylogeny‐based taxonomy. Burkholderia , Ralstonia , Cupriavidus , Pandoraea , Brevundimonas , Comamonas , Delftia , and Acidovorax species are aerobic, non‐spore‐forming, straight or slightly curved Gram‐negative rods. Several molecular genetic methods are available to assess the relatedness of isolates of these genera during nosocomial or community outbreak investigations. Of the organisms discussed in this chapter, serologic tests have been used clinically to diagnose only infections with B. pseudomallei . Given the uncertainty regarding the role(s) Delftia , Acidovorax , and Brevundimonas play in human infection, antimicrobial susceptibility testing of isolates from human specimens as a matter of routine may not be warranted.

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Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical systems and species-specific PCR

Cited by 10 publications

References 14 publications

Identification of Stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure

Identification of Stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure

In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain

Burkholderia , Stenotrophomonas , Ralstonia , Cupriavidus , Pandoraea , Brevundimonas , Comamonas , Delftia , and Acidovorax

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