Identification of serovar 1a, 1b, 2, and 5 strains of Erysipelothrix rhusiopathiae by a conventional gel-based PCR

Shiraiwa, Kazumasa; Ogawa, Yohsuke; Nishikawa, Sayaka; Eguchi, M.; Shimoji, Yoshihiro

doi:10.1016/j.vetmic.2018.09.014

Cited by 26 publications

(31 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The PCR-based typing scheme for serotypes 1a, 1b, 2 and 5 developed by Shiraiwa et al . [ 34 ] was implemented in silico by a blast + search of all primer sequences with the -task ‘blastn-short’ option active, requiring a full-length hit with 95 % similarity for a positive primer site identification. Isolates that did not match any of the targeted serotypes due to absent or inconsistent results were classified as non-typable (NT).…”

Section: Methodsmentioning

confidence: 99%

Comparative genome analysis of Erysipelothrix rhusiopathiae isolated from domestic pigs and wild boars suggests host adaptation and selective pressure from the use of antibiotics

et al. 2020

View full text Add to dashboard Cite

The disease erysipelas caused by Erysipelothrix rhusiopathiae (ER) is a major concern in pig production. In the present study the genomes of ER from pigs (n=87), wild boars (n=71) and other sources (n=85) were compared in terms of whole-genome SNP variation, accessory genome content and the presence of genetic antibiotic resistance determinants. The aim was to investigate if genetic features among ER were associated with isolate origin in order to better estimate the risk of transmission of porcine-adapted strains from wild boars to free-range pigs and to increase our understanding of the evolution of ER. Pigs and wild boars carried isolates representing all ER clades, but clade one only occurred in healthy wild boars and healthy pigs. Several accessory genes or gene variants were found to be significantly associated with the pig and wild boar hosts, with genes predicted to encode cell wall-associated or extracellular proteins overrepresented. Gene variants associated with serovar determination and capsule production in serovars known to be pathogenic for pigs were found to be significantly associated with pigs as hosts. In total, 30 % of investigated pig isolates but only 6 % of wild boar isolates carried resistance genes, most commonly tetM (tetracycline) and lsa(E) together with lnu(B) (lincosamides, pleuromutilin and streptogramin A). The incidence of variably present genes including resistance determinants was weakly linked to phylogeny, indicating that host adaptation in ER has evolved multiple times in diverse lineages mediated by recombination and the acquisition of mobile genetic elements. The presented results support the occurrence of host-adapted ER strains, but they do not indicate frequent transmission between wild boars and domestic pigs. This article contains data hosted by Microreact.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparative genome analysis of Erysipelothrix rhusiopathiae isolated from domestic pigs and wild boars suggests host adaptation and selective pressure from the use of antibiotics

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Immunological Marker E. rhusiopathiae isolates have historically been described by their serotype, which is based on testing for agglutination of an isolate against a panel of serotype-specific antisera using a double agargel diffusion method (Kucsera, 1973). It was recently determined that a chromosomal region encoding a putative pathway for polysaccharide biosynthesis is responsible for defining the antigenicity of the major serotypes 1a, 1b, 2 and 5, and that these can be distinguished based on their genetic composition at this locus Shiraiwa et al, 2018). However, this present study lends further support to the fact that serotype is inappropriate for assessing the genetic relatedness among isolates at larger scales (e.g.…”

Section: Serotype As An Epidemiological Andmentioning

confidence: 99%

Genomic and Immunogenic Protein Diversity of Erysipelothrix rhusiopathiae Isolated From Pigs in Great Britain: Implications for Vaccine Protection

et al. 2020

View full text Add to dashboard Cite

Frontiers in Microbiology | www.frontiersin.org March 2020 | Volume 11 | Article 418 Forde et al. Genomic Diversity of Erysipelothrix rhusiopathiaecommonplace for this bacterium. We hypothesize that the sequence variants in these proteins could be responsible for differences in the efficacy of the immune response. Our results provide the necessary basis for testing this hypothesis through in vitro and in vivo studies.

show abstract

“…Recently, we experienced an outbreak of erysipelas infection in egg‐laying chickens and found that it was caused by E. rhusiopathiae serovar 5 isolates (unpublished results). Serovar 5 strains have often been isolated from wild crows and wild boars in Japan . It is important to further investigate the role of wild boars in the spread and maintenance of E. rhusiopathiae in Japan.…”

Section: Prevalence (Positive Rate) Of Antibodies Against Erysipelothmentioning

confidence: 99%

“…Serovar 5 strains have often been isolated from wild crows and wild boars in Japan. 21 It is important to further investigate the role of wild boars in the spread and maintenance of E. rhusiopathiae in Japan.…”

mentioning

confidence: 99%

Wild boars: A potential source of Erysipelothrix rhusiopathiae infection in Japan

Shimoji

Osaki

Ogawa

et al. 2019

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

The potential role of wild boars as a source of erysipelas infection was investigated. An ELISA test of wild boar serum samples from 41 prefectures in Japan revealed that proportions of the Erysipelothrix rhusiopathiae‐positive samples were very high in all the prefectures, and the mean positive rate was 95.6% (1312/1372). Serovars of E. rhusiopathiae isolates from wild boars were similar to those of previously reported swine isolates, and all serovar isolates tested were found to be pathogenic to mice. These results suggest that wild boars in Japan constitute a reservoir of E. rhusiopathiae and may pose risks to other animals.

show abstract

Identification of serovar 1a, 1b, 2, and 5 strains of Erysipelothrix rhusiopathiae by a conventional gel-based PCR

Cited by 26 publications

References 24 publications

Comparative genome analysis of Erysipelothrix rhusiopathiae isolated from domestic pigs and wild boars suggests host adaptation and selective pressure from the use of antibiotics

Comparative genome analysis of Erysipelothrix rhusiopathiae isolated from domestic pigs and wild boars suggests host adaptation and selective pressure from the use of antibiotics

Genomic and Immunogenic Protein Diversity of Erysipelothrix rhusiopathiae Isolated From Pigs in Great Britain: Implications for Vaccine Protection

Wild boars: A potential source of Erysipelothrix rhusiopathiae infection in Japan

Contact Info

Product

Resources

About