Identification of sperm head proteins involved in zona pellucida binding

Petit, François; Serres, Catherine; Bourgeon, Frédéric; Pineau, Charles; Auer, J.

doi:10.1093/humrep/des452

Cited by 69 publications

(57 citation statements)

References 56 publications

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“…It has been reported recently that they might play a role in human fertility (Kwon et al 2013 ). Consistent with this proposal, VDAC2 was found to be one of the putative sperm head membrane proteins that bind ZP (Petit et al 2013 ).…”

Section: Voltage-dependent Anion Channels (Vdacs)supporting

confidence: 74%

Cl− Channels and Transporters in Sperm Physiology

Treviño

Orta

Figueiras-Fierro

et al. 2014

Sexual Reproduction in Animals and Plants

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Spermatozoa must decode environmental and cellular cues to succeed in fertilization, and this process relies heavily on ion channels. New observations bring to light the relevant participation of Cl − channels and anion transporters in some of the main sperm functions. Here we review the evidence that indicates the participation of Cl − channels in motility, maturation, and the acrosome reaction (AR), and what is known about their molecular identity and regulation. Our better understanding of sperm anion transport will yield tools to handle some infertility problems, improve animal breeding and preserve biodiversity, and develop selective and secure male contraceptives.

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Section: Voltage-dependent Anion Channels (Vdacs)supporting

confidence: 74%

Cl− Channels and Transporters in Sperm Physiology

Treviño

Orta

Figueiras-Fierro

et al. 2014

Sexual Reproduction in Animals and Plants

View full text Add to dashboard Cite

show abstract

“…Several methods of analysis have been developed to increase the identification of the low-abundance proteins on subcellular parts of the spermatozoa such as the head and flagellum (Cao et al 2006, Amaral et al 2013, Baker et al 2013, acrosomal matrix, , detergent-resistant membrane (Sleight et al 2005), phosphorylated proteins (Platt et al 2009), and proteins involved in capacitation (Ficarro et al 2003) and motility (Zhao et al 2007, Martinez-Heredia et al 2008, Chan et al 2009) and of sperm-egg interactions (Stein et al 2006, Petit et al 2013. In spite of the lack of a dynamic range of mass spectrometers to identify the low-represented proteomes, these 'shotgun' mass spectrometer approaches provide the opportunity to identify additional proteins involved in new or unexpected metabolic pathways for mature sperm (Amaral et al 2013).…”

Section: R31mentioning

confidence: 99%

“…Most of them have been developed using specific sperm antibodies (competitor or inhibitor) to prevent the binding of a candidate protein to the ZP or oocyte membrane. More global approaches have also been developed such as phage display technology (Naz 2005, Samoylova et al 2012, and the ZP affinity properties of sperm proteins have been analysed using different global and selective proteomic approaches (Stein et al 2006, Brewis & Gadella 2010, Belleannee et al 2011a, Petit et al 2013. Many protein candidates have been proposed following these in vitro studies, and more than 50 proteins have already been reported to be involved in sperm-egg interactions (Supplementary Table 1).…”

Section: Epididymal Surroundings and Sperm Proteins Involved In Egg-bmentioning

confidence: 99%

New insights into epididymal function in relation to sperm maturation

2014

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Testicular spermatozoa acquire fertility only after 1 or 2 weeks of transit through the epididymis. At the end of this several meters long epididymal tubule, the male gamete is able to move, capacitate, migrate through the female tract, bind to the egg membrane and fuse to the oocyte to result in a viable embryo. All these sperm properties are acquired after sequential modifications occurring either at the level of the spermatozoon or in the epididymal surroundings. Over the last few decades, significant increases in the understanding of the composition of the male gamete and its surroundings have resulted from the use of new techniques such as genome sequencing, proteomics combined with high-sensitivity mass spectrometry, and gene-knockout approaches. This review reports and discusses the most relevant new results obtained in different species regarding the various cellular processes occurring at the sperm level, in particular, those related to the development of motility and egg binding during epididymal transit.

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“…In addition, ZP2 and ZP3 can directly interact with voltagedependent anion channel 2 (VDAC2) (Petit et al, 2013). Because ion transport-based osmoregulation is the major function of the fish gill (Evans et al, 2005), we hypothesized that the expression of ZPs might be related to the ion transport of rare minnow gills, and this hypothesis constitutes the basis of our next investigation.…”

Section: Discussionmentioning

confidence: 98%