Identification of volatile metabolites in human saliva from patients with oral squamous cell carcinoma via zeolite-based thin-film microextraction coupled with GC–MS

Shigeyama, Hiroyo; Wang, Tao; Ichinose, M; Ansai, Toshihiro; Lee, SeungWoo

doi:10.1016/j.jchromb.2018.11.002

Cited by 67 publications

(30 citation statements)

References 49 publications

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“…[31] Shigeyama et al also revealed that 1-hexadecanol was newly produced in the saliva of oral squamous cell carcinoma patients, together with other alcohols and ketones as volatile metabolites. [32] Therefore, it seems likely that 1-hexadecanol was a candidate cancer-specific biomarker.…”

Section: Plos Onementioning

confidence: 99%

Identification of characteristic compounds of moderate volatility in breast cancer cell lines

et al. 2020

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In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H 2 O 2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles.

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Section: Plos Onementioning

confidence: 99%

Identification of characteristic compounds of moderate volatility in breast cancer cell lines

et al. 2020

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“…Benzer bir çalışmada, CCNB1 geninin yanında G2/M fazında görev alan CDC25C, CDC2, P53 ve fosforilize CDC2 genlerinin de anlatım düzeyleri tespit edilerek hücre döngüsünün aktive olma durumu açıklanmıştır. [1] Gelecek çalışmalarımıza bu genlerin dahil edilmesi, sunduğumuz bu ön bulguları netleştirecek sonuçların çıkartılmasına katkı sağlayacağını düşünmekteyiz.…”

Section: Discussionunclassified

“…Oral kanserler dünya genelinde meydana gelen kanserler arasında altıncı sırada yer almakta olup, oral yassı hücreli karsinomlar (OYHK) tüm oral kanserlerin %90'ını oluşturmaktadır. [1] Bununla birlikte, her yıl yeni 300,000 vakanın teşhis edildiği ve bunların 145,000'inin ölüm ile sonuçlandığı bilinmektedir. [2][3][4] OYHK'ların tedavisi evrelerine göre; cerrahi müdahale, radyoterapi/kemoterapi ya da bunların kombine uygulamaları olacak şekilde farklı stratejiler içermektedir.…”

Section: Introductionunclassified

Tinzaparin Tedavisinin Oral Yassı Hücreli Karsinoma Çıplak Fare Modelinde BAX ve CCNB1 Gen Anlatımları Üzerindeki Etkisi

Tuncer¹,

Ekici²,

Akça³

2018

Turk J Immunol

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Bu çalışma, oral yassı hücreli karsinom (OYHK) çıplak fare modelinde tinzaparin tedavisinin proapoptotik BAX ve hücre döngüsü G2/M faz regülatörü CCNB1 gen anlatım düzeyleri üzerindeki etkisini tespit etmeyi amaçlamıştır. Gereçler ve Yöntemler: Üç gruptan oluşan [tinzaparin tedavisi alan (n=4), pozitif kontrol (n=4), negatif kontrol (n=3)] 4-6 haftalık toplam 11 çıplak farede ortotopik OYHK fare modeli geliştirilmiştir. İnsan dil kanser hücre hattı OSC-19 bu in vivo modelin oluşturulmasında kullanılmıştır. Fareler 21 gün sonra sakrifiye edilerek, tümörler BAX ve CCNB1 gen anlatımları açısından kantitatif eş-zamanlı polimeraz zincir reaksiyonu (kRT-PZR) kullanılarak kantifiye edilmiştir. Gen anlatım düzeyleri Livak metodu ile ACTB housekeeping geni kullanılarak hesaplanmış ve kontrol örneklerine göre normalize edilmiştir. İstatistiksel anlamlılık non-parametrik Mann-Whitney U test ile hesaplanmış ve p değerinin 0.05'den küçük olması anlamlı olarak kabul edilmiştir. Bulgular: Pozitif kontrol ile tinzaparin gruplarından ikişer fare tümör geliştirmemiştir. Geri kalan tümörlerde kontrol grubuna kıyasla azalmış BAX ve CCNB1 gen anlatımı düşük istatistiksel anlamla tespit edilmiştir. Sonuçlar: Bu ön bulgular, apoptozun etkilenmediği, düzeni bozulmuş hücre döngü kontrolünü işaret etmektedir. Daha çok fare ile yapılacak çalışmalar tinzaparin tedavisinin OYHK fare modelindeki anlamlı etkisinin belirlenmesine yardımcı olacaktır.

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“…Furthermore, this study demonstrated increased analytical precision compared to their similarly developed ex vivo method, confirming the need for more rugged in vivo sampling devices for clinical settings. In 2019, Shigeyama and colleagues reported another application of TF-SPME by use of zeolite-based devices for the extraction of volatile organic compounds (VOCs) in saliva, a class of compounds that is used to determine if a patient has oral cancer [67]. This study again demonstrated the robustness of TF-SPME devices as a non-invasive method.…”

Section: Other Applications Of Tf-spmementioning

confidence: 90%

Development, Optimization and Applications of Thin Film Solid Phase Microextraction (TF-SPME) Devices for Thermal Desorption: A Comprehensive Review

2019

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Through the development of solid phase microextraction (SPME) technologies, thin film solid phase microextraction (TF-SPME) has been repeatedly validated as a novel sampling device well suited for various applications. These applications, encompassing a wide range of sampling methods such as onsite, in vivo and routine analysis, benefit greatly from the convenience and sensitivity TF-SPME offers. TF-SPME, having both an increased extraction phase volume and surface area to volume ratio compared to conventional microextraction techniques, allows high extraction rates and enhanced capacity, making it a convenient and ideal sampling tool for ultra-trace level analysis. This review provides a comprehensive discussion on the development of TF-SPME and the applications it has provided thus far. Emphasis is given on its application to thermal desorption, with method development and optimization for this desorption method discussed in detail. Moreover, a detailed outlook on the current progress of TF-SPME development and its future is also discussed with emphasis on its applications to environmental, food and fragrance analysis.

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Identification of volatile metabolites in human saliva from patients with oral squamous cell carcinoma via zeolite-based thin-film microextraction coupled with GC–MS

Cited by 67 publications

References 49 publications

Identification of characteristic compounds of moderate volatility in breast cancer cell lines

Identification of characteristic compounds of moderate volatility in breast cancer cell lines

Tinzaparin Tedavisinin Oral Yassı Hücreli Karsinoma Çıplak Fare Modelinde BAX ve CCNB1 Gen Anlatımları Üzerindeki Etkisi

Development, Optimization and Applications of Thin Film Solid Phase Microextraction (TF-SPME) Devices for Thermal Desorption: A Comprehensive Review

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