Identifikasi Dan Penetapan Kadar Flavonoid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers)

Marpaung, Mauritz Pandapotan; Wahyuni, Riska Choirunnisa

doi:10.32734/tm.v1i3.269

Cited by 13 publications

(11 citation statements)

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“…Pada penapisan fitokimia secara kualitatif, akar kuning mengandung senyawa metabolit sekunder berupa flavonoid, alkaloid, saponin, tanin, dan triterpenoid (Marpaung dkk, 2017). Secara kuantitatif, akar kuning mengandung flavonoid dengan kadar total sebesar 0,31031 ± 0,013607% (Marpaung & Wahyuni, 2018). Selain itu, akar kuning juga mengandung jenis saponin triterpenoid dengan kadar sebesar 4,51 ± 0,2805% (Wira & Marpaung, 2020).…”

Section: Pendahuluanunclassified

PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Wahyuni¹,

Marpaung²

2020

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Akar kuning (Fibraurea chloroleuca Miers) dengan senyawa metabolit sekunder yang terkandung di dalamnya merupakan salah satu tanaman yang berpotensi sebagai tanaman obat tradisional untuk mengobati berbagai penyakit. Tujuan penelitian ini adalah menentukan kadar alkaloid total ekstrak akar kuning dengan perbedaan konsentrasi pelarut etanol yaitu 50%, 70%, dan 96%. Proses ekstraksi menggunakan metode maserasi dan ekstrak diuapkan dengan rotary evaporator pada suhu 40oC. Identifikasi alkaloid pada ekstrak menggunakan pereaksi Wagner, Mayer dan Dragendorff dan kadar alkaloid total dilakukan dengan metode spektrofotometri UV-Vis. Hasil penelitian menunjukkan identifikasi alkaloid dengan semua pereaksi dinyatakan positif mengandung alkaloid pada ekstrak. Hasil kadar alkaloid total ekstrak akar kuning dengan pelarut etanol 50% sebesar 0,6939 ± 0,2439%, dengan pelarut etanol 70% sebesar 0,6607 ± 0,2117% dan pelarut etanol 96% sebesar 0,7826 ± 0,3004%. Berdasarkan hasil penelitian, ekstrak akar kuning dengan konsentrasi pelarut etanol 96% menunjukkan kadar alkaloid yang tertinggi.

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Section: Pendahuluanunclassified

PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Wahyuni¹,

Marpaung²

2020

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“…The Bi3+ ion from bismuth nitrate will react with potassium iodide to form a black precipitate of Bismuth (III) iodide which will then dissolve in excess potassium iodide to form tetraiodobismuthate. In this test nitrogen is used to form a coordinate covalent bond with K+ (Marpaung et al, 2017).…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Hepatoprotective Effectiveness Test of Salam Leaf Extract in Anthracyclin-Induced Rats

Halim

Girsang

Nasution

2022

IJHP

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Cancer is the most often diagnosed disease in the world and the top cause of death. Doxorubicin is a widely used systemic therapy, although its efficacy is limited by dose-related toxicity, including haematological suppression, organotoxicity, and hepatotoxicity. Bay leaf is a commonly utilised plant in the community for treating and preventing many diseases because it includes secondary metabolites that are antioxidants such as phenolics, alkaloids, saponins, steroids, triterpenoids, and tannins. The purpose of this work is to characterise EEDS, identify its secondary metabolite content, and test its nephroprotective effect in doxorubicin-induced rats using SGPT and SGOT levels and liver histology. This is a laboratory experimental investigation using rats and comprises of treatment groups receiving 0.5% of CMC-Na, Nature E, or EEDS at doses of 100 mg/kgBW, 300 mg/kgBW, or 500 mg/kgBW, respectively, followed by doxorubicin. The results indicated that EEDS had 21.64 % water soluble material, 1.79 % total ash, 9.98 % water, 45.38 % ethanol soluble extract, and 0.39 % acid insoluble ash. EEDS included alkaloids, flavonoids, tannins, glycosides, saponins, and steroids/triterpenoids, as determined by phytochemical screening. EEDS doses of 100 mg/kgBW, 300 mg/kgBW, and 500 mg/kgBW can significantly lower SGPT by 34%; 52,46%; 59,31% and SGOT levels by 27,05%; 43,96%; 50,07% and can improve the liver histopathology of doxorubicin-induced rats by reducing pyknotic degeneration, inflammatory cell infiltration, bleeding, steatosis and sinusoidal dilatation with an effective dose of 500 mg/kgBW with a significant difference with the doxorubicin group followed by 0.5% CMC Na. The key to bay leaf ethanol extract has hepatoprotective activity.

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“…Ke dalam masing-masing standar ditambahkan 1 ml AlCl3 2% dan 1 ml kaliu m asetat 120 mM. Semua campuran kemudian diinkubasi sampai stabil pada suhu kamar sebelum diukur absorbansinya menggunakan spektrofotometer UV-Vis dengan panjang gelombang maksimum 410 nm (Marpaung dan Wahyuni, 2018).…”

Section: Pembuatan Kurva Standar Kuersetinunclassified

PENENTUAN KADAR FLAVONOID DARI EKSTRAK KULIT SALAK (Salacca zalacca. Reinw) BERDASARKAN PERBEDAAN PENGERINGAN SIMPLISIA

Robbiyan

Pandapotan

Apriani

2021

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The skin of the thorny palm has been empirically recognized by some people as a traditional medicine for beauty, antimicrobial, and antidiabetic. Compounds that act as treatment in the skin of the thorny palm is flavonoids. The study aims to determine the total flavonoid of the thorny palm rind extract based on differences in the drying of simplicia. The simplicia drying method used was oven dry, direct sunlight, indirect sunlight, and air dry and fresh samples as control. Extraction using the maceration method with ethanol p.a. solvent. Determination of total flavonoid levels from the extract of thorny palm rind using the UV-Vis spectrophotometric method with quercetin standard solution. The qualitative test results showed that the skin of the thorny palm contains flavonoids after the addition of 1% AlCl3. The total flavonoid content in the fresh sample was 0.00342%, direct sunlight was 0.00350%, oven dry was 0.00340%, dry air was 0.00357%, and indirect sunlight was 0.00361%. Therefore, it can be concluded that the highest flavonoid content in the extract of thorny palm rind is found in the indirect sun drying method.

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Identifikasi Dan Penetapan Kadar Flavonoid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers)

Cited by 13 publications

References 1 publication

PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS

PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Hepatoprotective Effectiveness Test of Salam Leaf Extract in Anthracyclin-Induced Rats

PENENTUAN KADAR FLAVONOID DARI EKSTRAK KULIT SALAK (Salacca zalacca. Reinw) BERDASARKAN PERBEDAAN PENGERINGAN SIMPLISIA

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