PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Wahyuni, Septia; Marpaung, Mauritz Pandapotan

doi:10.31602/dl.v3i2.3911

Cited by 4 publications

(13 citation statements)

References 3 publications

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“…A total of 250 mg of caffeine was dissolved in hot distilled water and put into a 250 mL volumetric flask to obtain a concentration of 1000 ppm. Then 2.5 mL pipette was added and distilled water was added to a 25 mL volumetric flask to obtain a concentration of 100 ppm (Wahyuni and Marpaung, 2020).…”

Section: Analysis Of Total Alkaloid Levels With Uv-vis Spectrophotome...mentioning

confidence: 99%

“…The maximum wavelength of the caffeine solution was determined using a UV-Vis spectrophotometer at λ: 250-290 nm (Wahyuni and Marpaung, 2020).…”

Section: Determination Of the Maximum Wavelength (λMax) Of Caffeinementioning

confidence: 99%

“…Take 0.1; 0.3; 0.6; 0.9; 1,2; and 1.5 mL of 100 ppm caffeine standard solution and diluted to 10 mL so that the concentration of the standard solution is 1; 3; 6; 9; 12; and 15 ppm. Then measured the absorbance at the maximum wavelength using a UV-Vis spectrophotometer (Wahyuni and Marpaung, 2020).…”

Section: Caffeine Standard Curve Determinationmentioning

confidence: 99%

“…Weighed 10 mg of broccoli flower extract and dissolved to 10 mL, then shaken until homogeneous to obtain a concentration of 1000 ppm. Then pipette 1 mL of the sample and added ethanol up to 10 mL, then shaken until homogeneous to obtain a concentration of 100 ppm (Wahyuni and Marpaung, 2020).…”

Section: Preparation Of 100 Ppm Broccoli Flower Extract Mother Liquormentioning

confidence: 99%

“…Pipette 0.1 mL of the mother liquor extract and then put it into a 10 mL volumetric flask. Then 96% ethanol was added to the mark to make each extract concentration of 1 ppm (Wahyuni and Marpaung, 2020).…”

Section: Preparation Of Broccoli Flower Extract Solutionmentioning

confidence: 99%

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THE EFFECT OF MACERATION TIME ON TOTAL ALKALOID LEVELS IN BROCOLI (Brassica oleracea var. italica) BY USING UV-Vis SPECTROPHOTOMETRY METHOD

Sari¹,

Maimunah²,

Putri³

2022

jurnaljamukusuma

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ABSTRACT Broccoli is known to be very rich in secondary metabolites, such as alkaloids, flavonoids, tannins, polyphenols, essential oils, and polypeptides. Alkaloids are one of the secondary metabolites that are commonly found in nature and have physiological activities. The extraction time factor is the thing that can affect the quality of the extraction results which in this study is the alkaloid content. This study aims to determine the optimal maceration time so that it can produce optimum total alkaloid content. The stages in this study include sample preparation and then sample extraction by maceration using 96% ethanol with differences in maceration time of 1 day, 2 days and 3 days. Furthermore, identification using TLC and analysis of total alkaloid content using UV-Vis spectrophotometer. Kruskal Wallis test was used to determine whether there was an effect of maceration time on total alkaloid content. The results showed that the highest average total alkaloid content in the second sample was maceration for 2 days with a concentration of 17.5%. The Kruskal Wallis test showed that there was no effect between maceration time on total alkaloid content.

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Section: Analysis Of Total Alkaloid Levels With Uv-vis Spectrophotome...mentioning

confidence: 99%

“…The maximum wavelength of the caffeine solution was determined using a UV-Vis spectrophotometer at λ: 250-290 nm (Wahyuni and Marpaung, 2020).…”

Section: Determination Of the Maximum Wavelength (λMax) Of Caffeinementioning

confidence: 99%

Section: Caffeine Standard Curve Determinationmentioning

confidence: 99%

Section: Preparation Of 100 Ppm Broccoli Flower Extract Mother Liquormentioning

confidence: 99%

Section: Preparation Of Broccoli Flower Extract Solutionmentioning

confidence: 99%

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THE EFFECT OF MACERATION TIME ON TOTAL ALKALOID LEVELS IN BROCOLI (Brassica oleracea var. italica) BY USING UV-Vis SPECTROPHOTOMETRY METHOD

Sari¹,

Maimunah²,

Putri³

2022

jurnaljamukusuma

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IN VITRO ACUTE TOXICITY OF DANDELION LEAF EXTRACT (Taraxacum officinale F.H.Wigg) WITH BRINE SHRIMP LETHALITY TEST (BSLT) METHOD

Sugiyarto,

Muti,

Pradana

et al. 2023

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Toxicity is a characteristic feature of substances that can cause harmful effects. This study aimed to determine the toxicity of dandelion leaves (Taraxacum officinale F.H. Wigg) to Artemia salina Leach larvae within 24 hours. Dandelion leaves were extracted using a maceration method with 50% ethanol. The yield was 5.88%. The results showed that dandelion leaves contain alkaloids, flavonoids, steroids, triterpenoids, and phenols. Toxicity tests were performed using the Brine Shrimp Lethality Test method, where the final result was the LC50 value. Artemia salina Leach larvae were divided into five groups. Each group had ten larvae that underwent three replications. The 50%ethanolic extract of dandelion leaf concentrations in the treatment media were 50 ppm (P1), 100 ppm (P2), 150 ppm (P3), 200 ppm (P4), and 0 ppm (seawater) as the control. Probit analysis of Artemia Salina Leach mortality percentage data. The results indicated that the 50%ethanolic extract of dandelion leaves is harmful to larvae, with LC50 values of 165,223 ppm (toxic) or in the equation y = 2,114x + 0,311, with an R² value of 0,9435. Keywords: Artemia salina, BSLT, Dandelion leaf, LC50, Toxicity

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Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

Hanifah,

Daulay,

Rahman

et al. 2024

jharma

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The Bajakah tampala tree (Spatholobus littoral hassk) is a native plant from the interior of the forests in Kalimantan, especially Central Kalimantan. This plant is a plant that stretches or grows and grows abundantly in the interior of the forest. The Bajakah plant is known to the people of Kalimantan as a medicinal plant and can cure various diseases, including diarrhea, rheumatism, and cancer. The aim of this research was to determine the alkaloid content of bajakah root extract in various concentrations. The research method was carried out experimentally, with the independent variable being an ethanol extract of bajakah roots with concentrations of 50, 70, and 96%. Meanwhile, the dependent variables are the characteristics of simplicia, the secondary metabolite content of Bajakah root ethanol extract, and the determination of the total alkaloid content of Bajakah root extract. Bajakah root simplicia extract is made with 96, 70, and 50% ethanol solvents. The parameters used in this study include water content, total ash content, acid insoluble ash content, water soluble extract content, ethanol soluble extract content, and secondary metabolite content, including alkaloids, tannins, flavonoids, saponins, steroids, and terpenoids, by conducting phytochemical screening. , making a standard caffeine solution, determining the maximum wavelength of caffeine, measuring the caffeine calibration curve, and determining the total alkaloid content of Bajakah root extract. The results of the phytochemical screening showed that Bajakah root was positive for containing alkaloid compounds, saponins, tannins, flavonoids, steroids, and terpenoids. And the results obtained from Bajakah root plants obtained from Central Kalimantan were a 96% ethanol concentration of 1.66797 ± 0.7088 mg/gram, a 70% ethanol concentration of 1.6679 ± 0.1124 mg/gram, and a 50% ethanol concentration of 0.46981 ± 1.0656 mg/gram

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PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Cited by 4 publications

References 3 publications

THE EFFECT OF MACERATION TIME ON TOTAL ALKALOID LEVELS IN BROCOLI (Brassica oleracea var. italica) BY USING UV-Vis SPECTROPHOTOMETRY METHOD

THE EFFECT OF MACERATION TIME ON TOTAL ALKALOID LEVELS IN BROCOLI (Brassica oleracea var. italica) BY USING UV-Vis SPECTROPHOTOMETRY METHOD

IN VITRO ACUTE TOXICITY OF DANDELION LEAF EXTRACT (Taraxacum officinale F.H.Wigg) WITH BRINE SHRIMP LETHALITY TEST (BSLT) METHOD

Penentuan Kadar Alkaloid Total Ektstrak Akar Bajakah (Spatholobus Littoralis Hassk) dengan Metode Spektofotometri Uv-Vis

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