Identifying bracovirus and ichnovirus genes involved in virion morphogenesis

Lorenzi, Ange; Strand, Michael R.; Burke, Gaelen R.; Volkoff, Anne-Nathalie

doi:10.1016/j.cois.2021.11.006

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…Comparative analyses revealed a high degree of synteny between loci coding for DfIV and DsIV proviral segments, as well as flanking DNA. in other closely related PDV (Lorenzi et al, 2022). Furthermore, ichnoviruses and bracoviruses share a common life cycle but are morphologically different.…”

Section: Discussionmentioning

confidence: 99%

“…During oviposition, parasitoid wasps lay eggs containing a parasitoid-associated polydnavirus (PDV; Tanaka et al, 2007;Dicke et al, 2020). PDVs belong to the family Polydnaviridae, which comprises two genera, namely, Bracovirus and Ichnovirus (Stoltz and Vinson, 1979;Federici and Bigot, 2003;Lorenzi et al, 2022). The PDV genome comprises encapsidated double-stranded DNA and is 187-567 kb in length (Dupuy et al, 2006;Webb et al, 2006;Tanaka et al, 2007;Wang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Genome, host genome integration, and gene expression in Diadegma fenestrale ichnovirus from the perspective of coevolutionary hosts

Kim

Rahman²,

Kim³

et al. 2023

Front. Microbiol.

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Polydnaviruses (PDVs) exhibit species-specific mutualistic relationships with endoparasitoid wasps. PDVs can be categorized into bracoviruses and ichnoviruses, which have independent evolutionary origins. In our previous study, we identified an ichnovirus of the endoparasitoid Diadegma fenestrale and named it DfIV. Here, DfIV virions from the ovarian calyx of gravid female wasps were characterized. DfIV virion particles were ellipsoidal (246.5 nm × 109.0 nm) with a double-layered envelope. Next-generation sequencing of the DfIV genome revealed 62 non-overlapping circular DNA segments (A1–A5, B1–B9, C1–C15, D1–D23, E1–E7, and F1–F3); the aggregate genome size was approximately 240 kb, and the GC content (43%) was similar to that of other IVs (41%–43%). A total of 123 open reading frames were predicted and included typical IV gene families such as repeat element protein (41 members), cysteine motif (10 members), vankyrin (9 members), polar residue-rich protein (7 members), vinnexin (6 members), and N gene (3 members). Neuromodulin N (2 members) was found to be unique to DfIV, along with 45 hypothetical genes. Among the 62 segments, 54 showed high (76%–98%) sequence similarities to the genome of Diadegma semiclausum ichnovirus (DsIV). Three segments, namely, D22, E3, and F2, contained lepidopteran host genome integration motifs with homologous regions of about 36–46 bp between them (Diadegma fenestrale ichnovirus, DfIV and lepidopteran host, Plutella xylostella). Most of the DfIV genes were expressed in the hymenopteran host and some in the lepidopteran host (P. xylostella), parasitized by D. fenestrale. Five segments (A4, C3, C15, D5, and E4) were differentially expressed at different developmental stages of the parasitized P. xylostella, and two segments (C15 and D14) were highly expressed in the ovaries of D. fenestrale. Comparative analysis between DfIV and DsIV revealed that the genomes differed in the number of segments, composition of sequences, and internal sequence homologies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome, host genome integration, and gene expression in Diadegma fenestrale ichnovirus from the perspective of coevolutionary hosts

Kim

Rahman²,

Kim³

et al. 2023

Front. Microbiol.

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“…These results point out the practicality of RNA interference experiments for gene function prediction testing in V. canescens: for the first time, RNAi was performed in V. canescens and allowed to investigate the function of lef-4 and lef-8. In the same way as for Microplitis demolitor BV and Hyposoter didymator IV, for which RNAi allowed researchers to uncover endogenized viral gene functions (17,85,86), RNAi in V. canescens will allow us to test alphanudivirus gene predicted functions based on what is known for baculoviruses and bracoviruses and also to investigate the role of alphanudivirus genes with unknown functions. This research also opens the avenue for the investigation of cellular gene function in V. canescens.…”

Section: Discussionmentioning

confidence: 99%

Conserved Viral Transcription Plays a Key Role in Virus-Like Particle Production of the Parasitoid Wasp Venturia canescens

Araujo

Leobold

Bézier

et al. 2022

J Virol

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This study shows that endogenization of a nudivirus genome in a Campopleginae parasitoid wasp has led to the conservation, as for endogenized nudiviruses in braconid parasitoid wasps, of the viral RNA polymerase function, required for the transcription of genes encoding viral particles involved in wasp parasitism success. We also showed for the first time that RNA interference (RNAi) can be successfully used to downregulate gene expression in this species, a model in behavioral ecology.

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Functional characterization of Microplitis demolitor bracovirus genes that encode nucleocapsid components

Lorenzi,

Arvin,

Burke

et al. 2023

J Virol

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Bracoviruses (BVs) are endogenized nudiviruses in parasitoid wasps of the microgastroid complex (order Hymenoptera: Family Braconidae). BVs produce replication-defective virions that adult female wasps use to transfer DNAs encoding virulence genes to parasitized hosts. Some BV genes are shared with nudiviruses and baculoviruses with studies of the latter providing insights on function, whereas other genes are only known from nudiviruses or other BVs which provide no functional insights. A proteomic analysis of Microplitis demolitor bracovirus (MdBV) virions recently identified 16 genes encoding nucleocapsid components. In this study, we further characterized most of these genes. Some nucleocapsid genes exhibited early or intermediate expression profiles, while others exhibited late expression profiles. RNA interference (RNAi) assays together with transmission electron microscopy indicated vp39 , HzNVorf9-like2 , HzNVorf93-like , HzNVorf106-like , HzNVorf118-like , and 27b are required to produce capsids with a normal barrel-shaped morphology. RNAi knockdown of vlf-1a , vlf-1b-1 , vlf-1b-2 , int-1, and p6.9-1 did not alter the formation of barrel-shaped capsids but each reduced processing of amplified proviral segments and DNA packaging as evidenced by the formation of electron translucent capsids. All of the genes required for normal capsid assembly were also required for proviral segment processing and DNA packaging. Collectively, our results deorphanize several BV genes with previously unknown roles in virion morphogenesis. IMPORTANCE Understanding how bracoviruses (BVs) function in wasps is of broad interest in the study of virus evolution. This study characterizes most of the Microplitis demolitor bracovirus (MdBV) genes whose products are nucleocapsid components. Results indicate several genes unknown outside of nudiviruses and BVs are essential for normal capsid assembly. Results also indicate most MdBV tyrosine recombinase family members and the DNA binding protein p6.9-1 are required for DNA processing and packaging into nucleocapsids.

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Identifying bracovirus and ichnovirus genes involved in virion morphogenesis

Cited by 3 publications

References 40 publications

Genome, host genome integration, and gene expression in Diadegma fenestrale ichnovirus from the perspective of coevolutionary hosts

Genome, host genome integration, and gene expression in Diadegma fenestrale ichnovirus from the perspective of coevolutionary hosts

Conserved Viral Transcription Plays a Key Role in Virus-Like Particle Production of the Parasitoid Wasp Venturia canescens

Functional characterization of Microplitis demolitor bracovirus genes that encode nucleocapsid components

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