IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational

Curin, Mirela; Huang, Huey‐Jy; Garmatiuk, Tetiana; Gutfreund, Sandra; Resch, Yvonne; Chen, Kuan-Wei; Fauland, Kerstin; Keller, Walter; Zieglmayer, Petra; Zieglmayer, René; Lemell, Patrick; Horak, Friedrich; Hemmer, Wolfgang; Focke‐Tejkl, Margarete; Flicker, Sabine; Vrtala, Susanne; Valenta, Rudolf

doi:10.3389/fimmu.2021.687294

Cited by 18 publications

(23 citation statements)

References 45 publications

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“…New clinical trials of clinical relevance of certain HDM allergens are needed and the AIT optimizing strategies should be developed in order to increase its efficacy in patients with sensitization to Der p 5, Der p 7, and Der p 21, which is also suggested by other authors ( 9 ).…”

Section: Discussionmentioning

confidence: 86%

“…As Curin et al. state, patients with IgE antibodies to Der p 7 reported breathing difficulties more often and were more prone to asthma development, even though a mild one, than those patients who were not sensitized to Der p 7 ( 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…Other clinically significant HDM allergens are Der p 5, Der p 7, and Der p 21. What is more, according to the scientific literature, Der p 7 has the most expressed allergenic properties of all the allergens in the list ( 9 ). However, the data may be relevant only for the patients who participated in the clinical trial, as the pattern of sensitization is affected by several factors, namely, age of patients, the geographical region, poly- and mono-sensitization and also sensitization to certain allergen sources ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Profile Sensitization to House Dust Mites as an Important Aspect for Predicting the Efficiency of Allergen Immunotherapy

et al. 2022

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House dust mite (HDM) allergens are considered to be one of the most common causes of asthma and allergic rhinitis in the world. Cysteine proteases Der p 1 and Der f 1 (group 1) and also NPC 2 family proteins Der p 2 and Der f 2 (group 2) of D. pteronyssinus and D. farinae respectively are considered the main allergens of HDMs. The difference in the sensitivity of the population to these and other allergy causing components of HDM determines the treatment strategy. Thus, the purpose of this work was to determine the pattern of sensitization of the Ukrainian population to individual allergy causing molecular components of HDM in order to improve treatment strategies for the HDM allergy in various regions of Ukraine. To determine the molecular profile of sensitization to HDM, the data of multiplex allergy test Alex2 have been obtained from 10,651 patients. The sample included 57.86% children under the age of 18 and 42.14% adults. A Python language-based statistical analysis was performed, in order to group patients by sensitization to individual molecules and their combinations, regarding the age and geographical location of the patients. Simultaneous sensitization to Der f 2 and Der p 2 allergens was the most common among the entire group Simultaneous sensitization to 5 molecules—of group 1 (Der p 1 and Der f 1), group 2 (Der f 2 and Der p 2), and Der p 23—was the second most common for entire dataset and for the children group. This pattern differed in adults, where monosensitization to Der p 23 occupied the second position, suggesting that this molecule is an important factor of HDM allergy in Ukraine. Of the 16 analyzed regions, sensitization to Der p 23 prevailed in 2 Western regions of Ukraine. In the rest of the regions combination of Der p 2 and Der f 2 was the most prevalent. The established character of population sensitization to HDM in Ukraine is a good prognostic marker of allergen immunotherapy (AIT) efficacy.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Profile Sensitization to House Dust Mites as an Important Aspect for Predicting the Efficiency of Allergen Immunotherapy

et al. 2022

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“…The purity of the recombinant proteins and natural allergens was analyzed by SDS-PAGE (Figure S1). The molecular weight of the allergens was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, as previously described [47], and it was found to match the molecular mass predicted according to their sequences.…”

Section: Expression and Purification Of Allergensmentioning

confidence: 99%

Molecular Allergen-Specific IgE Recognition Profiles and Cumulative Specific IgE Levels Associated with Phenotypes of Cat Allergy

Riabova

Karsonova

Hage

et al. 2022

IJMS

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Cat allergy is a major trigger factor for respiratory reactions (asthma and rhinitis) in patients with immunoglobulin E (IgE) sensitization. In this study, we used a comprehensive panel of purified cat allergen molecules (rFel d 1, nFel d 2, rFel d 3, rFel d 4, rFel d 7, and rFel d 8) that were obtained by recombinant expression in Escherichia coli or by purification as natural proteins to study possible associations with different phenotypes of cat allergy (i.e., rhinitis, conjunctivitis, asthma, and dermatitis) by analyzing molecular IgE recognition profiles in a representative cohort of clinically well-characterized adult cat allergic subjects (n = 84). IgE levels specific to each of the allergen molecules and to natural cat allergen extract were quantified by ImmunoCAP measurements. Cumulative IgE levels specific to the cat allergen molecules correlated significantly with IgE levels specific to the cat allergen extract, indicating that the panel of allergen molecules resembled IgE epitopes of the natural allergen source. rFel d 1 represented the major cat allergen, which was recognized by 97.2% of cat allergic patients; however, rFel d 3, rFel d 4, and rFel d 7 each showed IgE reactivity in more than 50% of cat allergic patients, indicating the importance of additional allergens in cat allergy. Patients with cat-related skin symptoms showed a trend toward higher IgE levels and/or frequencies of sensitization to each of the tested allergen molecules compared with patients suffering only from rhinitis or asthma, while there were no such differences between patients with rhinitis and asthma. The IgE levels specific to allergen molecules, the IgE levels specific to cat allergen extract, and the IgE levels specific to rFel d 1 were significantly higher in patients with four different symptoms compared with patients with 1–2 symptoms. This difference was more pronounced for the sum of IgE levels specific to the allergen molecules and to cat extract than for IgE levels specific for rFel d 1 alone. Our study indicates that, in addition to rFel d 1, rFel d 3, rFel d 4, and rFel d 7 must be considered as important cat allergens. Furthermore, the cumulative sum of IgE levels specific to cat allergen molecules seems to be a biomarker for identifying patients with complex phenotypes of cat allergy. These findings are important for the diagnosis of IgE sensitization to cats and for the design of allergen-specific immunotherapies for the treatment and prevention of cat allergy.

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“…This step activates T cells and results in allergic inflammation. [11][12][13] Accordingly, a T cell-based vaccine has been tested and revealed the possibility of being a useful tool for attenuation of allergy, although the outcome was not remarkable.…”

Section: Allergen Types Used In Ait 1 Peptides and Modified Recombina...mentioning

confidence: 99%

New approaches to immunotherapy in house dust mite allergy

Kim

2023

Clin Exp Pediatr

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Allergen immunotherapy (AIT) has developed over the last few decades and has emerged as a promising treatment. House dust mite is a target allergen in AIT, and various modified house dust mite allergens have been improved for their efficacy. Moreover, clinical trials have proved their significantly therapeutic effects in allergy. This article review focuses on house dust mite allergens developed for AIT efficacy, and determines that their action mechanisms are strongly based on immune tolerance.Treatments for house dust mite allergens have been optimized by modification or/and addition of adjuvants, and clinically evaluated by subcutaneous and sublingual administration. Representative allergens used in AIT are chemically modified allergoids and recombinant allergens, including altered epitopes. The effective mechanisms subsequent to AIT include the suppressive actions of interleukin-10 and transforming growth factor-β secreted by regulatory cells, including regulatory T cells and regulatory B cells, and isotype switching from immunoglobulin E to immunoglobulin G4. Further studies of AIT are needed to achieve the milestone in treating allergies.

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IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational

Cited by 18 publications

References 45 publications

Molecular Profile Sensitization to House Dust Mites as an Important Aspect for Predicting the Efficiency of Allergen Immunotherapy

Molecular Profile Sensitization to House Dust Mites as an Important Aspect for Predicting the Efficiency of Allergen Immunotherapy

Molecular Allergen-Specific IgE Recognition Profiles and Cumulative Specific IgE Levels Associated with Phenotypes of Cat Allergy

New approaches to immunotherapy in house dust mite allergy

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