IgE‐, IgA‐ and IgG‐antibody responses to carbohydrate and protein antigens of Candida albicans in asthmatic children

Savolainen, J.; Viander, M.; Koivikko, A.

doi:10.1111/j.1398-9995.1990.tb01084.x

Cited by 51 publications

(56 citation statements)

References 16 publications

(27 reference statements)

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“…5). This confirmation was based on the reports in the literature (Strockbine et al, 1984;Walsh et al, 1988Walsh et al, , 1991Savolainen et al, 1990;Sundstrom and Aliaga, 1992;Mason et al, 1993;Ito et al, 1995;Chang et al, 2002). The enolase from the pathogenic yeast C. albicans has been identified as an immunodominant antigen in disseminated infection and in allergic disease (Mitsutake et al, 1994).…”

Section: Fungal Infection Of Vagina Is Sometimes Called a Thrush Cansupporting

confidence: 66%

“…For instance, in a study, serum samples from asthmatic patients reacted with fractions containing a 46 KDa protein (Walsh et al, 1991). The enzyme enolase from Saccharomyces cerevisiae and C. albicans acts as a major allergen and such allergen detected was believed to be enolase, a 46 KDa protein (Savolainen et al, 1990;Ito et al, 1995;Nittner-Marszalska et al, 2001;Kustrzeba-Wojcicka and Golczak, 2000;Chang et al, 2002). In S. cerevisiae the glycolytic enzyme, enolase has been localized as a 47 kDa protein (Pardo et al, 2000;Kim and Park, 2004).…”

Section: Fungal Infection Of Vagina Is Sometimes Called a Thrush Canmentioning

confidence: 99%

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Culture and Identification of Candida Albicans from Vaginal Ulcer and Separation of Enolase on SDS-PAGE

Bhavan¹,

Rajkumar²,

Radhakrishnan³

et al. 2010

IJB

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Candida albicans were isolated from patients with clinical symptoms of vaginal ulcer. Culture test for vaginal swab and scrapings were conducted on Sabouraud's dextrose broth and Sabouraud's dextrose agar plate respectively. Hichrome candida agar culture was used for differential identification of Candida. Smears from vaginal scrapings were prepared for gram staining. The suspected strain of Candida was inoculated on corn meal agar medium for chlamydospore formation. The suspected strain of Candida was inoculated in human serum for germ tube formation. Carbohydrate assimilation and fermentation tests were also conducted. The selected Candida colony was inoculated in YEPD medium for subculture and the cultured organism was harvested. The organisms were homogenized, centrifuged and the supernatant was filtered. The filtrate was extracted in chloroform. The extract was centrifuged and the aqueous phase was dialyzed. The dialyzed crude enolase was subjected to SDS-PAGE. The Sabouraud's dextrose broth inoculated with vaginal swab showed turbid growth. The scraping from vagina showed typical smooth creamy white colonies with a characteristic yeast odour on Sabouraud's dextrose agar plate. On Hichrome candida agar the Candida growth appeared as glistening green colored. Gram stained smears from vaginal scraps showed appearance of fungus as yeast January, 2010 85 budding. On corn meal agar the suspected Candida growth showed the formation of large, highly refractive, thick walled terminal chlamydospores. Germ tubes were seen as long tube like projections extending from the yeast cells on human serum inoculated with suspected strain of Candida. The carbohydrate assimilation tests were positive for dextrose, maltose, sucrose, galactose, xylose and trehalose, and negative for lactose, melibiose, ellobiose, inositol, reffinose and dulcitol. The carbohydrate fermentation tests showed positive for dextrose, maltose, galactose and trehalose, and negative for sucrose and lactose. SDS-PAGE for enolase from C. albicans showed a single polypeptide band of around 46 -48 kDa. International Journal of Biology

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Section: Fungal Infection Of Vagina Is Sometimes Called a Thrush Cansupporting

confidence: 66%

Section: Fungal Infection Of Vagina Is Sometimes Called a Thrush Canmentioning

confidence: 99%

Culture and Identification of Candida Albicans from Vaginal Ulcer and Separation of Enolase on SDS-PAGE

Bhavan¹,

Rajkumar²,

Radhakrishnan³

et al. 2010

IJB

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“…They originate from a wide range of animals, insects, plants, fungi or occupational sources. They are proteins or glycoproteins and more rarely glycans as in the case of Candida albicans (278).…”

Section: Allergen Exposurementioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,867

1,761

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“…Amount of nucleic acids was determined by Spirin's method, concentration of carbohydrates by Dubois method [23,24]. For studying of fractional structure and properties the method of protein separation by means of electrophoresis in polyacrylamide gel (PAAG) and affinity chromatography on Ni -activated sepharose was used [10,17,25].…”

Section: Methodsmentioning

confidence: 99%

“…At the present time there is no consensus of what components of the Candida cell are the main allergens. According to references, such main components of a cell wall of Candida albicans, as a polysaccharide mannan, mannoproteins and glucanoproteins, are the most allergenic [6][7][8][9], according to other literature sources -such secretory proteins as acidic protease with molecular mass of 31kDa and enolase [10]. It is obvious from different sources that different allergenic components of Candida yeast cause lesions of certain human body regions.…”

Section: Introductionmentioning

confidence: 99%

Obtaining and Experimental Study of Candida Allergens

Berzhets¹

2014

J Bacteriol Parasitol

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Due to the need of diagnostics of the widespread allergic diseases connected with infection ofa person by fungi, the question of creation of highly sensitive diagnostic panels of fungal allergens including molds and yeasts is important. Work purpose: creation of experimental technology of preparation of allergens from culture of C. albicans and studying of their physical, chemical and immunobiological properties. For receiving allergens we used the inactivated biomass of clinical strains of Candida albicans. Cultivation was carried out in the original mineral (without protein) CC1 and ML media in liquid and agarized variants with addition of sugar in various concentrations. In samples of allergens we defined amount of protein, carbohydrates, nucleic acids, specific activity in vitro (reaction of degranulation of rat mast cells). It was shown that the content of protein nitrogen varied from 72 to 18900 PNU, carbohydrates from 0,001 to 0,079 mg/ ml depending on physiological properties of a population of C. albicans and cultivation conditions. For determination of specific activity (reaction of rat mast cells degranulation) of samples used sera from patients with a sensitization to fungi. Allergenic activity of preparations in reaction of degranulation of rat mast cells varied from 3% (spontaneous) to 52%.The highest specific activity was shown for samples of the allergens obtained from C. albicans, grown in the ML medium. The results obtained using of 18 samples of preparations, can be a basis for a choice of an optimum technological mode of allocation of allergens from C. albicans.

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IgE‐, IgA‐ and IgG‐antibody responses to carbohydrate and protein antigens of Candida albicans in asthmatic children

Cited by 51 publications

References 16 publications

Culture and Identification of Candida Albicans from Vaginal Ulcer and Separation of Enolase on SDS-PAGE

Culture and Identification of Candida Albicans from Vaginal Ulcer and Separation of Enolase on SDS-PAGE

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Obtaining and Experimental Study of Candida Allergens

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